62 MARINE INGREDIENTS


120 100 80 60 40 20 0


Control * * **


300 250 200 150 100 50 0


0.6 1.8 % of xPL


Figure 1: Release (%) of ATP in fibroblasts treated for 48 hours (*p<0.05; **p<0.01 versus control)


more efficiently, and maintain a healthier, more radiant appearance as well as healthy longevity.


Material and methods Source and composition An extract of the red alga Polysiphonia lanosa (L.) T.A.Chr, referred to here as xPL, was produced, standardized for digeneaside content and tested for its beneficial effects on skin.


In vitro studies The application of xPL on normal human keratinocytes (NHK) and fibroblasts (NHF) in several biological models showed that it protects the lysosomal and mitochondrial networks in NHK and NHF and stimulates mitophagy and autophagy activities in skin cells, thus preserving cell and tissue longevity.


6 Control ** * **


0.6


1.8 % of xPL


Figure 2: Autophagic activity (%) in fibroblasts treated with xPL 48 hours (MDC probe, *p<0.05; **p<0.01 versus control)


Boosting cell energy and regeneration Fibroblasts were treated with xPL for 48 hours after which the ATP (the cellular energy molecule) content was measured and the cells were labelled with the monodansyl cadaverine (MDC) probe to assess autophagic activity. The increase in the synthesis of ATP (Figure 1) is dose-dependent, showing a direct effect of the extract on the cell energy production. At a concentration of 1.8%, xPL boosts the synthesis of ATP by 46%. 1.8% of xPL stimulates up to 20% increases in autophagic activity (Figure 2).


Protecting cell integrity To demonstrate the protective effects of xPL on cell integrity, we preventively applied xPL for 24 hours, then submitted the mitochondrial and lysosomal networks of keratinocytes and


fibroblasts to UVA irradiation. xPL was reapplied for 24 hours, after which the mitochondrial and lysosomal networks were stained with MitoTracker or LysoTracker to visually reveal the effects. UVA irradiation disrupted both mitochondrial


and lysosomal networks, reflecting oxidative damage and impaired cellular function. Treatment with xPL at 1.8% preserved the organization and integrity of these cellular structures, confirming its protective effect against oxidative stress (Figure 3).


Building a stronger skin matrix To evaluate its effect on skin structure, human dermal fibroblasts isolated from a 55-year-old donor were treated with 2% xPL for 48 hours. The production of three key extracellular matrix biomarkers: collagen 1, elastin and hyaluronic acid was then quantified using ELISA assays.


6


Figure 3: Effect of xPL on Mitochondrial & Lysosomal networks in NHK and NHF cells exposed to UVA irradiation Mitochondrial NHF


Mitochondrial NHK Lysosomal NHF PERSONAL CARE MAGAZINE October 2026


Lysosomal NHK www.personalcaremagazine.com


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76  |  Page 77  |  Page 78  |  Page 79  |  Page 80  |  Page 81  |  Page 82  |  Page 83  |  Page 84  |  Page 85  |  Page 86  |  Page 87  |  Page 88  |  Page 89  |  Page 90  |  Page 91  |  Page 92  |  Page 93  |  Page 94  |  Page 95  |  Page 96  |  Page 97  |  Page 98  |  Page 99  |  Page 100  |  Page 101  |  Page 102  |  Page 103  |  Page 104  |  Page 105  |  Page 106  |  Page 107  |  Page 108  |  Page 109  |  Page 110  |  Page 111  |  Page 112  |  Page 113  |  Page 114  |  Page 115  |  Page 116  |  Page 117  |  Page 118  |  Page 119  |  Page 120  |  Page 121  |  Page 122  |  Page 123  |  Page 124  |  Page 125  |  Page 126  |  Page 127  |  Page 128  |  Page 129  |  Page 130  |  Page 131  |  Page 132