118 EYE AREA CARE
epidermal barrier. The same model was used to evaluate
pigmentation. Melanin content decreased to 9.84% of control values at 10 µg/mL (data not shown). This result is relevant because pigmentation, vascular components and shadowing co-contribute to the clinical perception of dark circles. Reducing melanin accumulation in an eyelid-relevant model supports the peptide’s role in improving tone uniformity. Dermal structure was assessed through
markers involved in extracellular matrix organization and dermal-epidermal junction integrity. Collagen XVIII increased to 176.3% of control values and collagen I to 133.7% (Figure 3). Emilin-1, a key component of the elastic microfibrillar network, exceeded 900% of control values (data not shown), suggesting enhanced dermal resilience and extracellular matrix organization. 4,5 These data indicate that the peptide does not
act solely on vascular and lymphatic pathways. It also improves key parameters of eyelid skin quality, including barrier function, pigmentation balance and dermal matrix structure.
Remodelling underlying support tissues: adipose tissue, muscle and ligament structures The underlying tissues of the periocular region have a major influence on visible eye contour architecture. Adipose tissue plays an important role in eye bag formation and periocular volume changes. In primary human adipocyte models, the AI-designed peptide reduced lipid accumulation during adipocyte differentiation to 77.3% of control values. In already differentiated adipocytes, lipid
accumulation decreased to 62.7% of control values, indicating activity both on adipogenesis-related
A Young injured control Aged injured control
Aged injured +10 ug/mL AI-designed peptides
C Young injured control Aged injured control
Aged injured +10 ug/mL AI-designed peptides
160 140 120 100 80 60 40 20 0
* -35.0%
Ceramides Control
Collagen type I Control
Collagen type XVIII Control
10 µg/mL AI-designed peptide
10 µg/mL AI-designed peptide
10 µg/mL AI-designed peptide
Ceramides in red, cell nuclei in blue
Collagen type I in red, cell nuclei in blue
Collagen type XVIII in red, cell nuclei in blue
Figure 3: Biological effect on epidermal ceramides, collagen type I and collagen type XVIII in a 3D full thickness reconstructed pigmented eyelid skin model. Ceramides, collagen type I and collagen type XVIII were assessed by immunostaining and quantified by image analysis
lipid accumulation and on lipid content in mature adipocytes (data not shown). Periocular muscle is also central to eyelid position and the appearance of tired eyes. Loss of muscle tone and mitochondrial activity during ageing can contribute to eyelid drooping. In an aged muscle model, treatment increased active mitochondria content to 132.6% of aged control values (Figure 4). In a blepharoptosis-like aged muscle model including satellite cells, IGF-1 levels reached 135% of aged control values, supporting increased regenerative capacity (Figure 4). These findings connect cellular energization and muscle regeneration with the visible improvement of the
upper eyelid area.6–9 Ligament-support structures remain an
important yet underexplored component of facial architecture. In aged human ligament cells, the AI-designed peptide promoted the expression of extracellular matrix-related markers associated with structural resilience while reducing the expression of stress-associated genes (data not shown). At the protein level, collagen I increased to 353% of aged control values, supporting improved extracellular matrix organization and structural resilience. These results support the concept that meaningful eye contour care should not stop at the skin surface. The function and resilience of
B
Young injured control
Aged
injured control
Aged injured + 10 µg/mL
Eyeseryl All-In peptide
Figure 4: Effect of the AI-designed peptide on muscle regenerative capacity and cellular energy. (A) Muscle cells were injured by mechanical scratching and IL-1β treatment to simulate blepharoptosis (abnormal drooping of the upper eyelid) and treated in the presence or absence of the AI-designed peptide. Cells were visualized by fluorescence microscopy. (B) IGF-1 levels were quantified in culture supernatants. Statistical significance was determined using Student’s t-test (*p < 0.05). (C) Active mitochondria content in an aged muscle cell model was assessed by fluorescence microscopy using a specific fluorescent probe
PERSONAL CARE MAGAZINE October 2026
www.personalcaremagazine.com
IGF-1 levels (%)
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