SKIN MICROBIOME 47 15 13.77*** 11.62** 10.74 10 -71% 5 Control Normal Silabskin® RE Control


Water-stressed Silabskin®


RE


Figure 4: Effect on Transepidermal water loss (TEWL). ***: significant result according to Student’s t test/normal Silabskin®


provides an assessment of the integrity of the epidermal barrier function. When it is altered, water flow towards the exterior intensifies, increasing the value of TEWL. When subjected to a water stress, Silabskin RE exhibits a significant increase of transepidermal water loss (+28%). TEWL was measured with a Tewameter®


.


When the product is tested at 0.2% in this model, epidermal dehydration is significantly limited (-71%)(Fig 4). Through this modelling study, the postbiotic was proven to boost the mechanisms inherent in establishing an effective barrier function, thereby limiting the effects of epidermal dehydration, with the improvement of differentiation process and epidermal cohesion and the activation of the lipid cement formation. These actions at the cell level are supporting cosmetics benefits.


Effect of a postbiotic on skin dryness The effect of the postbiotic on dry skin was studied in vivo on male and female volunteers by determining its capacity to leave the cutaneous microbiota untouched, to boost the renewal of the epidermal barrier, and consequently, to improve the quality of dry skin.


Preservation of the equilibrium of the microbiota In order to determine the effect of the ingredient on the cutaneous microbiota, samples were taken on the forehead of a panel of 18 healthy Caucasian volunteers, who were selected for having dry skin on the hands and face. They applied the product formulated at 1% in an emulsion for 28 days. More precisely, the experts from the in-


February 2020 RE control (P <0.01) **: significant result according to Student’s t test/water-stressed Silabskin® RE control (P <0.01) 0.2% Lactobiotyl®


cutaneous microbiota (Fig 5). Activation of epidermal renewal The capacity of the postbiotic to improve cell renewal was conducted on 14 healthy Caucasian volunteers with dry skin on the hands and face. The measurement was made on parameter b*, characteristic of yellow melanin skin pigmentation, previously induced by treatment with dihydroxyacetone (DHA). Assessment was done on photos of the inner side of the forearms with a C-Cube Clinical Research dermoscope. After 14 days of twice-daily application and in comparison to the placebo, the active formulated at 1% in an emulsion accelerates cutaneous cell renewal by more than 20% (gain of 3 days over 14 days of treatment).


Improvement of hydration and complexion radiance


Evaluation of hydration


house microbiota platform sequenced the V1-V2 variable regions of the gene for 16S ribosomal RNA (16S rDNA), shared by all bacteria, with the MiSeq®


system, and


analysed the following parameters:  Alpha diversity, showing diversity in the sample;


 Taxonomy, that assesses in relative abundance the composition and distribution of bacterial communities. These analyses descended to the level of the genus.


In the conditions of this study, after 28


days of treatment, the ingredient does not affect the microbiota of dry skin: no significant effect was observed on diversity, composition or distribution of the


The hydrating benefit of the postbiotic was measured on two panels of Caucasian and Asian volunteers:  The Caucasian panel was composed of 19 healthy volunteers having applied the ingredient and the placebo to the top of the hands four times a day;


 The Asian panel was composed of 31 healthy volunteers having applied the ingredient and the placebo to each half of the face in the morning and evening. Measurement of hydration was done with a on the hands of the Caucasian


Corneometer®


panel and the cheeks of the Asian panel. After 28 days of application and in comparison to the placebo, the postbiotic formulated at 1% in an emulsion significantly increases skin hydration on the hands of Caucasian volunteers (+16.2%, P < 0.01) (Fig 6) and on the face of Asian


100


80


60


40


20


0 D0 D28 + 1% Lactobiotyl®


 Acinetobacter  Anaerococcus  Corynebacterium  Cutibacterium  Enhydrobacter  Haemophilus  Paracoccus  Prevotella  Staphylococcus  Streptococcus  Other


Figure 5: Effect on the distribution of bacterial communities (metasequencing). PERSONAL CARE EUROPE


TEWL (g/h/m2


)


Relative abundance (%)


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