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46 SKIN MICROBIOME Silabskin® RE


Normal Control


Silabskin® RE


Water Stress Control


Silabskin® RE Water Stress +0.2% Lactobiotyl®


1,144 AU


862*** AU


1,138** AU +98%


***: significant result/normal Silabskin® RE control (P < 0.01) **: significant result/water stressed Silabskin® RE control (P < 0.01) Figure 2: Effect on the synthesis of filaggrin.


They are cyclic polyols, osmoregulating molecules known to play a fundamental role in adjusting osmotic pressure in plants. In drought conditions, they participate in maintaining the water status of the plant, in turn contributing to preserving its active metabolism. Such metabolites present a suitable molecular profile to treat skin dryness.


A 3D model mimicking dry skin Dry skin is a common disorder that is marked by a number of symptoms including discomfort, excessive desquamation and the appearance of unsightly irregularities. These manifestations are the result of an alteration of the skin barrier function, caused by a number of factors (deterioration of the epidermal differentiation and cohesion, defect in the formation of the lipid cement and transepidermal water loss). With the implementation of an in-house modelling mimicking dry skin, the company has been able to demonstrate the alteration of the skin barrier function and to prove that its active ingredient reactivates its integrity. More precisely, this was done by developing a novel in vitro 3D model of reconstructed epidermis (Silabskin®


RE) subjected to a


water stress, induced by incubation in an atmosphere of 40% humidity (vs. 82%) for 3 consecutive days.


Epidermal differentiation and cohesion The skin barrier protects the body from external stresses, whether physical, chemical or environmental. It also acts against dehydration by limiting the diffusion of water to the exterior of the body. A multitude of protein components is indispensable for creating this barrier, among which are filaggrin, claudin-4 and desmoglein-1. When Silabskin RE is subjected to a water


PERSONAL CARE EUROPE 65** 60


stress, epidermal differentiation and cohesion are altered, characterised by a significant reduction in the syntheses of filaggrin (-25%), claudin-4 (-28%) and desmoglein-1 (-23%). This study was conducted with immunohistofluorescence. Tested at 0.2% in this model, the postbiotic significantly restores the synthesis of filaggrin (+98%) (Fig 2), claudin-4 (+97%) and desmoglein-1 (+163%).


Formation of the lipid cement Intercorneocyte lipids play a basic role in the skin barrier function. The lipid matrix is synthesised primarily by keratinocytes. Its composition and specific organisation ensure the tightness of the stratum corneum and regulate the flow of electrolytes from the interior to the exterior of the body and vice- versa. Their syntheses and secretions involve


180 +297% 120 100 169*** +242% 100 74* +244% 137*** 100 73* 139***


several players, including glucosylceramidase (GBA), fatty acid synthase (FASN) and ATP binding cassette subfamily A member 12 (ABCA12).


When Silabskin RE is subjected to a water


stress, there is a significant decrease of enzymes synthesising intercorneocyte lipids, as well as of a protein that transports them (GBA: -35%; FASN: -26%; ABCA12: -27%). This study was conducted by quantitative PCR. Tested at 0.2% in this model, the postbiotic significantly restores the expressions of GBA (+297%), FASN (+242%) and ABCA12 (+244%) (Fig 3).


Transepidermal water loss Transepidermal water loss (TEWL) is the passive diffusion of water through the stratum corneum. Its measurement


0 GBA  Normal Silabskin® RE Control


Water-stressed Silabskin® Water-stressed Silabskin® Lactobiotyl®


RE Control RE + 0.2%


*: significant result/normal Silabskin® **: significant result/normal Silabskin®


***: significant result/water-stressed Silabskin® Figure 3: Effect on the synthesis and transport of epidermal lipids. February 2020


RE control (P < 0.05) RE control (P < 0.01) RE control (P < 0.01)


FASN ABCA12


Expression (%)


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