EQUIPMENT & ACCESSORIES CATALOG EDITION V CRYO-SEM & CRYO-FIB/SEM PREPARATION SYSTEMS


zzzCryo Transfer Systems (continued) ORDERING INFORMATION


For a full quotation, including on-site installation and customer training, please contact us. Cat. No.


Description PP3004 QuickLok Ambient Temperature Transfer System, includes:


Qty. each


Airlock assembly. Pump and vent and transfer controls, valve and fittings to the pumping system (see: Pumping below). Custom designed interface flange to the microscope vacuum chamber Microscope dovetail stage to accept specimen shuttle. LED chamber light (interlocked) Specimen transfer device for vacuum or inert gas transfer


PP3005


Specimen holders. Specimen shuttle with holding clips, specimen shuttle blank, specimen shuttle (to hold a 10mm dia. specimen stub), blank 10 mm stubs – packet of 10 SEMCool Non-Airlock Low Temperature System, includes:


each


Nitrogen gas cooled cold stage with heater and sensor and cold trap with temperature sensor. Temperature controllable with a range down to -190°C, 21 L liquid nitrogen dewar with trolley, heat exchanger and LED chamber light. Pump fittings (see: Pumping below). Temperature and nitrogen gas flow controller mounted on the dewar trolley. Specimen holders. 3 specimen shuttles (to hold 10 mm Ø cryo stubs), blank specimen shuttle, specimen shuttle with holding clips, blank 10 mm Ø stubs (packet of 10), 5 multi-purpose specimen stubs. Note: other holders available


PP3006


Specimen mounting compounds (colloidal graphite and Tissue-Tek® CoolLok Cryo Transfer System, includes:


Airlock assembly. Pump and vent and transfer controls, ) each gate valve and fittings to the pumping


system (see: Pumping below). Custom designed interface flange to the microscope vacuum chamber. Cooling system. Nitrogen gas cooled cold stage with heater and sensor and cold trap with temperature sensor. Temperature controllable with a range down to -190°C, 21 L liquid nitrogen dewar with trolley, heat exchanger and LED chamber light. Specimen transfer device


Specimen holders. 3 specimen shuttles (to hold 10 mm Ø cryo stubs), blank specimen shuttle, specimen shuttle with holding clips,blank 10 mm Ø stubs (packet of 10), 5 multi-purpose specimen stubs. Note: other holders available Specimen mounting compounds (colloidal graphite and Tissue-Tek®


Pumping


The PP3004 QuickLok and PP3006 CoolLok require either a rotary pump or high vacuum turbomolecular pumping station (recommended). The PP3005 requires a rotary pump for evacuating the vacuum isolated gas lines. 13034 24426


Pfeiffer Duo 6 — 5 m3


Options and Accessories 24429


PP7450 13296


/hr rotary vacuum pump with oil mist filter Pfeiffer HiCube 80 turbomolecular and diaphragm pumping system


each each


Rapid cooling station (for PP3006 only). Consists of a floor-mounted trolley, liquid nitrogen freezing chamber mounted into the work surface which interfaces to the cryo transfer device, connections to vacuum pump (order separately)


Pressurized (60L) LN2 dewar. Boil-off nitrogen gas is used for cooling the stage and cold trap (PP3005 and PP3006 only)


Sircal in-line gas dryer. Helps to reduce water content of nitrogen gas supply


Specimen Holders 10245 10246 10247 E7433


E7449-5 E7401 E7402 E7403 E7405 E7406 E7407


32816510


Top-loading specimen shuttle for planchettes Top-loading specimen shuttle, to take a 10mm stub Top-loading specimen shuttle for rivets (vice style)


Rivet holder specimen stub, screw-down style (for use with 10246) Universal specimen stub with surface holes and slots (5 pack) Specimen stub shuttle (spare) Aluminum (Al) stubs (10 pack) Copper (Cu) stubs (10 pack)


Screw down stub for thin, hard specimens Copper (Cu) stubs with 3 x 3mm slots (5 pack) Copper (Cu) stubs with 1 x 3mm slot (5 pack) Brass rivets for fracturing liquids (100 pack)


Sputter Targets and Carbon Fiber E7400-314A Gold (Au) target 0.008" thick


E7400-314B Gold/palladium (Au/Pd) (80:20) target 0.2mm thick E7400-314C Platinum (Pt) target 0.008" thick E7400-314IR Iridium (Ir) target 0.008" thick E7400-314CR Chromium (Cr) target 0.3mm thick Carbon fiber cord — high purity — 1m Carbon fiber cord — high purity — 5m


91047-1 91047-5


each each


each


each each each each each each each each each each each each


each each each each each each each


), interlock cable and pump fittings


The SEM image shows amyloplasts from a high moisture content (72 – 82%) potato tuber (Solanum tuberosum). Prepared using the Quorum PP3010 cryo-preparation chamber. Preparation involved cryo-immobilization at -207 °C in slush nitrogen, fracturing at -140 °C, and sputtering with 4 – 6 nm of Iridium. A sublimation period of 6 min. was used for the tuber specimen to reveal both the amyloplasts and cellulose cell wall compartments. Imaged on Tescan Amber.


The SEM image shows pollen from a pumpkin stamen prepared using the PP3010 preparation chamber. A section of stamen was taken and mounted using mounting media (50:50 mixture of Tissue-Tek OCT (Optimal Cutting Temperature) compound and colloidal graphite), vitrified in slushed nitrogen (LN2) at 207 °C, fracturing at -140 °C, sublimed to -90°C for 2 min and sputter coated with 4– 7 nm of Iridium. Imaged on Tescan Amber.


The micrograph shown here is of Hydrated Sodium Polyacrylate Hydrogel. The sample itself was sourced from dyed sodium polyacrylate hydrogel beads. The bead was cut into a hemisphere using an exacto knife and mounted using a mixture of tissue tek and colloidal graphite (70:30 ratio) onto a cryo stub. The sample was then plunged freezed in LN2, fractured, sublimated and coated with 5 nm of Au/Pd using the PP3010 cryo system. This sample was then loaded onto a Quorum cold stage and imaged within a Zeiss Evo. A beam energy of 6.00kV was used with a probe current of 16 pA.


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