EQUIPMENT & ACCESSORIES CATALOG EDITION V GLOW DISCHARGE SYSTEMS zzzGloQube® Plus (continued) The primary application of the GloQube® Plus is the hydrophilization (wetting) of carbon-coated TEM


support films and grids which otherwise have the tendency to be hydrophobic. Glow discharge treatment with air will make film surfaces negatively charged and hydrophilic and allow the easy spread of aqueous solutions. This and other processes are outlined below.


Glow Discharge Typical Processes Surface State


Hydrophilic Hydrophilic Hydrophobic** Hydrophobic** Air


Positive Positive


Negative Air* Alkylamine Methanol * Air followed by post-treatment with magnesium acetate by the user. ** Hydrophobic, as noted above, may represent a less hydrophilic sample of less than 90 degrees contact angle.


Contact angle left: positive discharge using methanol shows carbon grid less hydrophobic Contact angle right: negative discharge using amylamine shows carbon grid surface hydrophobic


Hydrophilic Hydrophiobic


Surface Charge Atmosphere Typical Applications Negative


Recommeded applications for the GloQube®


Plus:


• Hydrophilization and cleaning of TEM grids carbon support films* for better sample spreading


• Improved adhesion and orientation of proteins, nucleic acids and antibodies


Hydrophilisation and cleaning of carbon coated TEM grids


Nucleic acid adhesion to carbon films


Controlled orientation and improved adhesion of negatively charged proteins, antibodies and nucleic acids


Controlled orientation and improved adhesion of positively charged protein molecules (e.g. ferritin, cytochrome c)


• TEM grid preparation for nanoparticle studies


These products are for Research Use Only.


* Typically: Formvar® , Lacey Carbon, Holey Carbon, Continuous Carbon, Quantifoil®


TEM images of the 20S human proteasome complexes, showing the effect of altering the surface charge of the carbon support film on the orientation of the protein. 2.5 nm of carbon film on Quantifoil®


1.2/1.3 400 mesh was used as a support for the sample.


Carbon surface without glow discharge treatment.


Visible some side views of the protein due to uneven charge of the surface.


Carbon surface with air glow discharge biased top view orientation of the protein caused by uniform negative charge of the surface.


Biased-top views 20s proteasome complex adsorption on in-air glow discharge modified carbon support TEM grids.


9


Carbon surface with amylamine glow discharge.


Biased side-view orientation of the protein caused by uniform positive charge on the surface.


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