Pre-Meeting Sunday doi:10.1017/S1431927611000675
Short Courses: August 7, 2011 Organizer: Mike Marko ~Additional Fees are Required!
• These full-day courses run from 8:30 AM to 5:00 PM on Sunday, August 7, 2011. • Morning and afternoon coffee breaks are provided; lunch is on your own from on-site vendors. • Additional registration fees apply; see M&M 2011 website for details. • A certificate of participation will be issued to each participant.
Biological Sciences
X-10 CRYO-PREPARATION FOR BIOLOGICAL EM ORGANIZER: KENT MCDONALD 8:30 AM–5:00 PM, ROOM 102
In this course we will briefly review why cryo-techniques for specimen preparation are superior to conventional meth- ods.We will discuss some low-cost-alternative cryo-methods, as well as demonstrate some of the latest equipment and techniques for high pressure freezing, plunge freezing and cryosectioning, CryoSEM, and freeze fracture. The Toku- yasu method for immunolabeling will also be covered briefly. Persons taking this course should leave with a better under- standing of these cryotechniques and their role in different applications such as EM tomography, vitreous cryosection- ing, EM immunolabeling, as well as routine use for the best available preservation of cellular fine structure.
X-11 IMMUNOLABELING TECHNOLOGY FOR LIGHT AND ELECTRON MICROSCOPY ORGANIZER: CAROLINE MILLER
8:30 AM–5:00 PM, ROOM 103
The requirements for successful immunohistochemical and immunocytochemical labeling vary widely with different biological systems. The optimal techniques for light- microscope labeling often differ greatly from those needed for electron microscopy. The basics of immunolabeling at the light and electron microscope level will be presented, illustrated with examples from several different biological systems. The course will cover specimen preparation,multi- ple labeling, immunogold labeling and enhancement meth- ods, and correlative LM/EM techniques.
22
X-12 LIVE CELL IMAGING USING FLUORESCENCE METHODS ORGANIZERS: SIMON WATKINS AND CLAUDETTE
ST.CROIX 8:30 AM–5:00 PM, ROOM 104
Microscopic imaging tools are one of the principal method- ologies applied to the living system. This day-long work- shop concentrates on live cell imaging using fluorescence methods, focusing on optimization of the entire microscope system. The goal is to collect the highest quality, most robust quantitative data without perturbing the cells being imaged. Lectures on the fluorescent proteins will be pre- sented as well as discussions of the merits of newer methods such as TIRF and multiphoton imaging.
X-13 BASIC CONFOCAL LIGHT MICROSCOPY ORGANIZERS: JAY JEROME AND BOB PRICE 8:30 AM–5:00 PM, ROOM 105/106
Confocal microscopy has become a primary method for visualizing structure in three dimensions. The technology is rapidly evolving with new instruments, lasers, detectors, and spectral imaging capabilities. Bob and Jay will instruct beginning and intermediate researchers on carrying out successful biological confocal microscopy experiments. Em- phasis will be on practical aspects of specimen preparation, instrument setup and operation, and enhancement and analysis of the digital images collected by confocal micros- copy. A general knowledge of optical microscopy is helpful, but no prior knowledge of confocal microscopy is necessary to benefit from the workshop.
Microscopy Microanalysis
AND © MICROSCOPY SOCIETY OF AMERICA 2011
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120 |
Page 121 |
Page 122 |
Page 123 |
Page 124 |
Page 125 |
Page 126 |
Page 127 |
Page 128 |
Page 129 |
Page 130 |
Page 131 |
Page 132 |
Page 133 |
Page 134 |
Page 135 |
Page 136 |
Page 137 |
Page 138 |
Page 139 |
Page 140 |
Page 141 |
Page 142 |
Page 143 |
Page 144 |
Page 145 |
Page 146 |
Page 147 |
Page 148 |
Page 149 |
Page 150 |
Page 151 |
Page 152 |
Page 153 |
Page 154 |
Page 155 |
Page 156 |
Page 157 |
Page 158 |
Page 159 |
Page 160 |
Page 161 |
Page 162 |
Page 163 |
Page 164 |
Page 165 |
Page 166 |
Page 167 |
Page 168 |
Page 169 |
Page 170 |
Page 171 |
Page 172 |
Page 173 |
Page 174 |
Page 175 |
Page 176 |
Page 177 |
Page 178 |
Page 179 |
Page 180 |
Page 181 |
Page 182 |
Page 183 |
Page 184 |
Page 185 |
Page 186 |
Page 187 |
Page 188 |
Page 189 |
Page 190 |
Page 191 |
Page 192 |
Page 193 |
Page 194 |
Page 195 |
Page 196 |
Page 197 |
Page 198 |
Page 199 |
Page 200 |
Page 201 |
Page 202 |
Page 203 |
Page 204