This page contains a Flash digital edition of a book.
BIOSAFETY


‘To properly design a laboratory that meets specific standards of work, it is necessary to know the biosafety level that will be adopted.’


from 3.00 m to 3.60 m wide by 6.00 m to 9.00 m long is recommended, with the entrance located at the narrowest end. The width is scaled according to the


movement of people and the depth of the benches and equipment. Modules that are longer than 9 m may have their functionality compromised because of the difficulty of accessing the tanks, biosafety cabinets and equipment. The recommended width for movement between benches varies from 1.20 m to 1.40 m.


Figure 1: Emergency shower and eyewash.


These units can contain a variety of services such as refrigerators, incubators, freezers, centrifuges, water bath, microscope and handling systems. They cannot contain gas.


Emergency shower and eyewash An emergency shower and eyewash is necessary in case of accidents in the laboratory. They must be installed in an easily accessible location with drive levers that can be operated by hand, elbow or a floor platform. It should have controlled pressure and the shower head needs to be around 30cm in diameter. The eyewash should consist of two small showers of medium pressure, coupled to a metallic bowl, whose angle allows for the correct direction of the water jet into the face and eyes. Emergency showers and eyewashes should


be installed in all laboratories, especially in BL-3 laboratories. Emergency showers can be installed in areas of movement, without the obstruction of the passage and near access door to the laboratory. Eyewash stations can be installed in sink countertops or are coupled together in emergency showers as shown in Figure 1.


Design guidelines for BL-3 laboratories The construction, renovation or adaptation to the physical structure of laboratories should be preceded by approval of the project by the local health authority, in Brazil this also needs to be in accordance with ANVISA, the Brazilian Health Surveillance Agency. Planning for construction of new laboratories should consider a lifespan of 10-20 years, and five years for renovation projects when plans include new facilities. For standardisation of building elements, and possible reductions in cost and time to completion a modular system is necessary. As a basic laboratory module, a rectangle


IFHE DIGEST 2014


Circulation and flow Circulation and flow must meet accessibility standards and emergency exits. Predicting a minimum width of 1.20 m for movement of laboratory staff, the lanes of material, equipment and personnel need to have a minimum width of 2.00 m for lengths greater than 11.00 m and 1.50 m wide for others. Laboratory flows for staff, clean and soiled


materials need to be well defined in the project design, to minimise risks in the workplace.


Walls and panels Dividing walls can be incorporated into the laboratory to meet the need for flexibility. The panels should be coated in a washable, clear or frosted material with non-porous and smooth contours. It is mandatory that BL-3 laboratory masonry walls are properly sealed and coated in a washable material that is chemical resistant, light coloured with a smooth contour and rounded corners.


Floors The floors should be levelled, with the fewest possible joints, and should be of a non- porous and non-slip coated material. They need to be easy to clean and disinfect and should also be chemical resistant. For BL-3 areas it is mandatory to use a monolithic epoxy flooring that is waterproof and non-slip with a smooth contour and that is resistant to both gases and chemicals.


Ceilings Ceilings must have a solid, continuous seal to prevent contaminants from infiltrating roof spaces that may be difficult to access. Continuous ceilings are compulsory in laboratory environments – sealed and waterproof, demoted or not, mesh reinforced gypsum or equivalent material coated washable, non-porous, resistant to chemicals and gases and without recesses. In other areas – such as circulation, technical, administrative and support – recesses for removable plates are recommended that can be used as acoustic material.


Doors


The door frames need to be of a material that can be easily cleaned and maintained. Viewers are mandatory in partition walls between rooms and circulation and in doors between rooms and passageways. The minimum width of access doors into laboratory environments, washing, sterilsation and warehouses is 1.10 m and 0.80 m in other laboratory areas. Doors need


Figure 2: Location of the exhaust ducts for biological safety. 49


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68  |  Page 69  |  Page 70  |  Page 71  |  Page 72  |  Page 73  |  Page 74  |  Page 75  |  Page 76  |  Page 77  |  Page 78  |  Page 79  |  Page 80  |  Page 81  |  Page 82  |  Page 83  |  Page 84  |  Page 85  |  Page 86  |  Page 87  |  Page 88  |  Page 89  |  Page 90  |  Page 91  |  Page 92  |  Page 93  |  Page 94  |  Page 95  |  Page 96  |  Page 97  |  Page 98  |  Page 99  |  Page 100