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New Independent Validation Study Shows Rapid Automated System is as Accurate as Manual Colony Counting
Synbiosis is delighted to announce its ProtoCOL 3 rapid automated colony counter has been shown in an independent study to perform with the same level of accuracy as manual colony counting for enumerating 10 different types of microbial colonies on a range of agar plates. The study, which was performed to GLP-compliant standards at Don Whitley Scientific Contract Microbiology Laboratory, compared the ProtoCOL 3 system with manual counting for enumeration of bacterial, yeast and fungal colonies on either Plate Count Agar, Columbia Blood Agar or Sabouraud Dextrose Agar plates. These plates were surface spread or spiral plated with one of the following organisms: Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Kocuria rhizophila, Enterococcus faecalis, Mannheimia haemolytica, Bacillus subtilis, Streptococcus pneumoniae, Candida albicans and Aspergillus brasiliensis. The resulting colonies were then enumerated both manually and using the ProtoCOL 3’s powerful software to produce a count.
For each plate type (spiral and spread) the comparison between ProtoCOL 3 and manual counts were analysed statistically using a t-test. The results (p = 0.105 for spiral plate data and p = 0.143 for spread plate data) did not identify significant differences between manual and the automated counting methods, for either plate type, at the 95% confidence level.
Martin Smith at Synbiosis commented: “The microorganisms in this independent study produce colonies of differing colours, shapes and sizes and were also cultured on both translucent and opaque agars, which means some are a real challenge to count automatically. We’re delighted with the results of this study because they show that there is no significant difference between the accuracy of manual and automated counting with the ProtoCOL 3 in what are realistic evaluation situations you’d see in many microbiology laboratories.” Martin continued: “Being able to accurately enumerate so many types of colonies on different agars is a task very few automated colony counters can perform well and this study validates the ProtoCOL 3’s versatility for this application. Microbiologists looking to increase their throughput of plate counts can now install a ProtoCOL 3, confident that they will automatically count many different types of bacteria, fungi and yeast in a fraction of the time, while still guaranteeing the accuracy they demand from a manual count.”
MORE INFO. 208
India Lab Expo 2013
Patented Technology Automatically Checks Microplate Washer Performance
BioTek’s 405™ Touch and 405 LS Microplate Washers are now available with patented Verify™ Technology – the easiest, fastest and most reliable way to identify clogged washer manifold tubes. Verify uses an ultrasonic sensor to identify specific clogged dispense and aspirate tubes that can lead to inconsistent washing and assay failure.
Using Verify, a pre-programmed quality control routine fills microplate wells with liquid and confirms the fluid levels. Microplate wells are then aspirated, and the fluid levels are measured again. Any discrepancy in the fluid level indicates a blockage, commonly caused by salt crystal and protein buildups, and the user is alerted to the precise well location. The 405’s integrated Ultrasonic Advantage™ may then be used to eliminate the clogs using powerful ultrasonic technology.
India Lab Expo 2013 will take place on 21st-23rd November Hitex Exhibition Center, Hyderabad, India. India Lab Expo has become the single meeting point by excellence for the laboratory fraternity in India.
Show organisers will not only be welcoming their prestigious exhibitors but also count a number of new companies to exhibit for the first time. Many foreign companies are interested to appoint new distributors in different part of India.
This year the focus areas are Pharmaceutical, Research, Education, Food, Medical Lab, Environment and Material Testing labs. It appears that the 5th edition will continue the success with at least 400+ exhibitors.
New at India Lab Expo are India Diagnostic Expo and Pharma Mac Pac Expo which are co-located events on Medical Lab and Pharmaceutical Machineries respectively.
India Lab Expo 2012 achieved its 4th milestone successfully in Hyderabad. The three day technology show was biggest in South East Asia for laboratory, analytical, biotechnology and scientific instruments. Exhibitors have got excellent response and 91% have achieved their primary purpose of participation.
The total number of exhibitors was 250 in two different halls in an exhibition area of 9000m2
. The
laboratory industry gathered for the fourth time to open its gates to more than 7,000 trade visitors of which 3000 were from biotechnology, pharmaceutical & food industries, and rest from research institutes, academia, trade suppliers and different labs.
The Bio Lab Analytika 2012 conference co-located with the show based on laboratories guidelines, analysis & management and gathered 550 delegates from around the world to be a knowledge sharing platform for everyone.
For more information email:
info@indialabexpo.com MORE INFO. 210
New Mini Centrifuge with Big Benefits
Labnet International is proud to introduce their new personal mini centrifuge, the PRISM MINI. Reliable and easy-to-use, the Prism Mini is compact and economical, allowing each workstation to be equipped with a ‘personal’ centrifuge. The Prism Mini can be used for a wide range of molecular biology separations and quick spins.
The centrifuge was designed to be extremely compact with an exceptionally small footprint. It includes 2 quick release interchangeable rotors, which spin up to 6000 RPM’s. The electronic brake and self opening lid allow for quick deceleration to reduce handling time. The Prism Mini’s maintenance-free drive is remarkably quiet and the unique design allows for excellent airflow, to protect temperature-sensitive samples.
MORE INFO. 212
The optional Verify module is available on several BioTek 405 Touch and 405 LS models. The 405 Touch Microplate Washer provides 96- and 384-well compatible protocol creation and implementation via a simple, LED touch-screen interface, with automated internal buffer switching, quick release manifolds and optimised cell washing, even for loosely adherent cell monolayers. Additionally, two built-in USB ports enable individual file storage, transfer and operation. The 405 LS model incorporates many of the same features as the 405 Touch, and uses a keypad interface for remote operation or robotic installations.
Both the 405 Touch and 405 LS are powered by Liquid Handling Control™ (LHC™) software, and vacuum filtration and biomagnetic separation versions are available.
MORE INFO. 209
New Rotors for Centrifuges Introduced
Eppendorf introduces four new rotors for Centrifuges 5804/5804 R and 5810/5810 R, which are defined as the true workhorses of the lab with their renowned quality, reliability and versatility. Two new swing-bucket rotors increase the capacity of Centrifuge 5804/5804 R to 1 litre and Centrifuge 5810/5810 R to 3 litre.
The new 3 litre swing-bucket rotor (4 x 750mL) makes the Centrifuge 5810/5810 R the smallest 3 litre centrifuge available and contains options for round and plate buckets, either of which can use new aerosol-tight Eppendorf QuickLock®
bucket design allows for energy savings up to 36% (compared to rotor A-4-81).
In addition, there are two new fixed-angle rotors now available for both Centrifuges 5804/5804 R and 5810/5810 R. These rotors include aerosol-tight Eppendorf QuickLock lids, which require only a quarter turn!
The new 48 x 1.5/2mL rotor (FA-45-48-11) has a max speed of 19,083 x g. Also available is a high-capacity fixed-angle rotor for 15mL conical and glass tubes (F-35-48-17), with capacity for 40 x 15mL conical tubes and max speed up to 5,050 x g.
MORE INFO. 211 caps for one-handed operation. Also, the new aerodynamic round
INTERNATIONAL LABMATE - JANUARY/FEBRUARY 2013
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