Sample Preparation & Processing
Flexible Kit Helps Speed and Simplify Nucleic Acid Size Selection for Fragment Library Prep
Stir Temperature-Controlled Liquid Samples Directly on the Laboratory Bench CoolHeater®
bench. The CoolHeater®
It is compact and Replaces bulky refrigerator equipment, ice and water baths. The instrument is precise and provides temperature control from 0 to 99°C. CoolHeater® flexibility - beaker and up to 5 Eppendorf test tubes at the same time.
provides great
Application examples include: mixing of heat-sensitive substances such as enzymes or proteins, dialysis directly on the lab bench; re-crystalisation; long-term reactions at constant temperatures or organic synthesis in micro-scale.
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magnetic stirrers from Implementa Hebe AB offers easy, temperated stirring right on top of your lab magnetic stirrers are intended for users in Biotech, LifeScience amongst other areas.
CoolHeater® is convenient as it cools, heats and stirs liquid samples directly on the laboratory bench.
SPRIselect from Beckman Coulter Life Sciences utilises SPRI (Solid Phase Reversible Immobilisation)-based chemistry to speed and simplify genomic DNA size selection for next generation sequencing fragment library preparation. Following shearing, the library construction process requires size selection to produce uniform distribution of fragments. SPRIselect allows size distribution to be adjusted between 150 and 800 base pairs to suit the application and sequencing platform. The process can be performed manually or automated for high throughput in 96-well plates on platforms such as Beckman Coulter Life Sciences’ Biomek Workstations. SPRIselect reagent kits are available in 5, 60 and 450mL volumes, enable rapid and consistent size selection, and come with guidelines to assist users in customising protocols. Gel cartridges, chips and additional instruments are not needed. Samples should be fragmented, double-stranded DNA of 50mL or greater, dissolved in molecular biology grade water or such standard buffer solutions as Tris or TE.
“This will be enormously valuable to researchers seeking to increase accuracy, efficiency and throughput in gDNA size selection for next-gen sample prep,” said Julie Moore, Director of Marketing, Beckman Coulter Life Sciences Automation and Genomics. “Scientists will find that SPRIselect enhances both research processes and results in sequencing labs.” SPRIselect is ideal for use in most common next generation applications, including paired-end, single-end, targeted, ChiP and RNA sequencing, and for use with samples to be run on such platforms as Illumina’s MiSeq, HiSeq and Genome Analyzer IIx; Roche’s 454 GS FLX+ and GS Junior; and Life Technologies’ SOLiD series and Ion Torrent PGM.
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Fast Homogenisation with Reproducible Results
No matter whether it is in medical and pharmaceutical biotechnology, they all have one thing in common: sample analysis. In the preceding sample preparation, efficient solubilising of material systems and reproducible results combined with easy handling is a prerequisite.
The handheld homogeniser, POLYTRON® PT1200E, from Kinematica, homogenises samples quickly,
easily and with reproducibility. The highest degree of efficiency means sample heating is kept to a minimum. It is manufactured from high-quality biotechnical stainless steel available in Ø 3, 5, 7 and 12mm.
The technology: small, light, easy to use; high-performance and speed rotation ranges; durable technology; change assemblies quickly using click and go; washing out the assemblies is quick and easy using the EasyClean Design; high-quality Swiss-made quality.
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Convenient and Efficient Cryogenic Grinding
For size reduction of sample materials which cannot be processed at room temperature, Retsch’s CryoMill is the ideal choice. An integrated cooling system ensures that the grinding jar is continually cooled with liquid nitrogen before and during the grinding process.
Thus the sample is embrittled and volatile components are preserved. An Autofill System provides liquid nitrogen in the required amount so that the user never gets into direct contact with the LN2 which makes operating the mill particularly safe.
The new CryoMill achieves considerably improved grind sizes thanks to the increased oscillating frequency of 30 Hz. Another new feature is the possibility to store up to 9 SOPs.
Further improvements include a reinforced housing, optimised liquid nitrogen duct and new accessories such as an adapter for 6 reaction vials and a 10 ml grinding jar. The mill can also be operated without cooling which makes it suitable for a vast range of applications.
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Reactors with Ultimate Mixing Performance Offer Simpler Scale-Up
Asynt Ltd in conjunction with Innovative Physical Organic Solutions (IPOS), a research group based within the University of Huddersfield, UK, have cooperated over the last 2 years to develop a new generation of chemical reactor.
By better understanding and improving the flow and thermal dynamics of the Asynt ReactoMate system the co-operation is able to now produce reactors that offer ultimate mixing performance. The benefits of this development for chemists will be simpler process development and scale-up, faster reactions, better reproducibility and improved selectivity.
Martyn Fordham, Managing Director of Asynt Ltd, commented: "We were pleased to cooperate with IPOS on this development because of their considerable expertise in chemical engineering and experience in advising chemists on scaling reactions that were often rate limited by poor mixing." He added: "At the start of our cooperation, Asynt ReactoMate reaction systems, as with all of our competitors, did not offer optimised mixing. Today however, we can offer customers the option to use ReactoMate to scale chemistries with well designed mixing by IPOS. This consideration has proven especially important when reaction chemistries are exothermic for example."
Dr Nicholas Powles, a Senior Research Fellow at IPOS commented "The complexities associated with optimising and scaling-up laboratory reactions are of critical importance in industrial process development. Our co-operation with Asynt will help commercialise the essential tools and 'know-how' for efficient process understanding and optimisation."
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