38 SKIN MICROBIOME
500 450 400 350 300 250 200 150 100 50 0
Control (untreated) Figure 3:Oxytocin levels in NHE.
plant cell culture-derived ingredient and produce an in situ postbiotic that significantly increases the synthesis of oxytocin by the skin keratinocytes and sensory neurons. This postbiotic is produced by the commensal microbiota locally (in situ) and directly on the skin and stimulates the skin cells to ignite the synthesis of positive neurochemicals. Those ‘excited’ keratinocytes could stimulate the skin nerve endings and connect the skin directly with the brain with the aim of stimulating and reinforcing a positive loop of communication between the skin and the brain that ends up in a skin with a better health and appearance. Considering the great impact of
emotions and wellbeing on the skin homeostasis and appearance, it is essential to consider the microbiota-skin-brain axis in this new cosmetic strategy (Fig 1).
The concept of in situ postbiotic Postbiotic is a relatively new term coined to refer to the metabolic by-products of probiotic bacteria. It is widely recognised that the use of different kinds of prebiotics, probiotics and postbiotics in dermocosmetics due to its contrasted
ROI T0d T28d (mm2Area )
Length (mm)
0.003% KANNABIA SENSEPLF 3-fold 0.8 8-fold 0.6 0.4 0.2 0.0
0.003% bSN
Control (untreated)
0.003% bSN
Figure 4:Oxytocin levels in sensory neurons.
beneficial effects on both skin cells and microbiota. The concept of in situ postbiotic is
based on a simple fact: who better than our own commensal symbiotic microbiota to take care of our skin? The skin microbiota continuously captures nutrient elements from its surroundings, transforms them and generates an in situ postbiotic mantle, rich in bioactive metabolites that can interact with the epidermal cells and sensory neuron nerve endings. This approach profits from the high
metabolisation of skin commensal microbiota to obtain a cocktail of neuroactive by-products. The cocktail of neuroactive by-products significantly ignites the synthesis of happiness related neurochemicals (oxytocin) from skin cells by metabolising a plant stem cell derived product.
The first Cannabis sativa stem cells The research around the biotechnological culture of Cannabis sativa stem cells redefines the concept of cosmetics and incorporates a transgressive vision of ‘emotional cosmetics’.
Before After Vol.#6 The scientific evidence of our studies
shows the great capacity of cellular factors from Cannabis sativa stem cells to connect in a multidimensional cascade with the microbiota-skin-brain axis and from which oxytocin plays a crucial role in the way in which the individual relates to others. Thus it reaches the emanated beauty, which goes beyond the results conventionally obtained through skin nutrition. In other words, depending on the reaction generated (oxytocin production), the individual and skin appearance may change. Based on plant cell culture technology,
this new active ingredient opens a new paradigm in the field of well-ageing: use the microbiota to increase the skin happiness neurochemical networks in order to enhance the feeling of comfort and self- confidence. Through the new active Kannabia
SensePLF , it is possible to stimulate the skin
microbiota to produce an in situ post-biotic cocktail that stimulates the synthesis of cutaneous oxytocin. The oxytocin generated in the keratinocytes connects with the sensory neurons and communicates with the brain turning into a well-being response that ends up in a healthier, stronger, and more beautiful appearance. This active (INCI name: Cannabis Sativa
Frown
Marionette lines
Figure 5: Clinical evaluation of wrinkles. PERSONAL CARE EUROPE
Callus Lysate) is the first active made from Cannabis sativa plant stem cells and has been obtained through the proprietary Technology Platform Phyto-Lipidic Fractions. It has been enriched with a great and diverse cocktail of plant terpenes and polyphenols while avoiding the production of the controversial cannabinoids THC and CBD. Once metabolised by the skin commensal microbiota, this synergic cocktail can stimulate the synthesis of wellbeing related neurochemicals (oxytocin) through an in situ produced postbiotic.
In vitro efficacy Several in vitro tests were performed to demonstrate the modulation of the skin microbiota in situ postbiotic thanks to the active.
June 2020 1% kSN
Oxytocin levels (pg/ml)
Oxytocin levels (relative to BCA)
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