Sample Preparation & Processing Positive Customer Commentary for Automated Evaporation System


Genevac has reported very positive feedback from early adopters of their Rocket 4D – a fully automated system for automatic drying or concentration of very large volumes up to 100 litres. A chemistry scale-up laboratory at a major Pharma company, running kilo scale preparative SFC, were drawn by the unattended automation offered by the Rocket 4D. Traditionally removing large volumes of methanol from their sample fractions using a number of rotary evaporators required a dedicated operator to top-up dry ice in cold traps, feed the systems with more product and watch the systems continually. The operator commented: “With the Rocket 4D you simply load your sample, select a method, press start, and walk away – the system will do the rest.”


A natural products research group, at a US Ivy League university, were faced with concentrating large volumes of aqueous extracts from vegetable tubers. The university selected the Rocket 4D as it was easiest to operate, had technology to prevent sample bumping and did not require users to handle and clean large heavy glass evaporation vessels.


Compact in size the Rocket 4D allows you to dry or concentrate your samples with complete confidence as it uses proprietary vacuum technology to suppress solvent bumping and foaming.


The Rocket 4D comes as standard with a single, 5 litre 316 stainless steel vessel for drying or concentrating product. Access to the dried or concentrated product in the vessel is very easy. Dried products can be scooped out, or where enhanced user protection is needed, re-dissolved while the vessel is still in the system. Liquid products can be easily drained via a drain port in the side of the rotor, using a dedicated pouring stand. Users wishing to dry volumes larger than five litres require the ‘Autofeed’ option, which enables the Rocket 4D to draw in product from the user’s own external supply. The Rocket 4D controls product feed, drying and the discharge of condensed waste solvent without any external intervention. The autofeed system has an integrated rinse circuit which enables dried products to be automatically re-dissolved in a small volume of solvent at the end of the process. Cleaning the Rocket 4D between cycles is also very straightforward.


28201pr@reply-direct.com ChIP Enzymatic Shearing Kit


Porvair Sciences (UK) has announced a new Chromatrap Enzymatic Shearing Kit that contains all the necessary reagents and buffers for up to 10 chromatin preparations.


The success of a ChIP assay is highly dependent on the quality of chromatin prepared. The Chromatrap enzymatic shearing kit allows you to determine optimal shearing conditions for your chromatin preparations and can supply you with enough chromatin to perform up to 24 ChIPs if using standard Chromatrap ChIP spin column kit or up to 96 IP’s if using Chromatrap ChIP 96 High throughput microplate.


Chromatin immunoprecipitation (ChIP) is a technique used to study the association of specific proteins,or their modified isoforms, with defined genomic regions. In a ChIP assay, DNA-protein complexes (Chromatin) are identified using matching antibodies and the resulting fractions treated to separate the DNA and protein components. Polymerase Chain Reaction (PCR), Real Time PCR, hybridisation on microarrays, or direct sequencing are typically used to identify DNA fragments of defined sequence.


The Chromatrap ChIP assay developed by Porvair is more efficient than conventional bead based method as the solid phase porous polymer functionalised with either protein A or G is more efficient in capturing chromatin. Also it uses a spin column approach, offering significant advantage over the sepharose or magnetic beads. The levels of DNA pull down with Chromatrap is about 25 times more than conventional methods, and the signal to noise ratios for DNA enrichment is about 2 to 3 times better even with samples anywhere between 50ng to 3000ng chromatin per immunoprecipitation.


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Save Time Optimising Process Conditions Enabling Rapid Vaccine Production


TAP Biosystems (UK) have announced it has developed a new method of using microcarriers with its ambr micro bioreactor system. This technique enables scientists to produce uniform microcarrier samples and perform automated media exchange to rapidly optimise parameters for culturing adherent cell lines on microcarriers, thus shortening timelines for successfully scaling up manufacturing of vaccines and cell therapies.


Scientists at TAP have utilised the ambr workstation to develop an automated method for providing highly consistent dispensing of microcarriers to multiple 10-15mL ambr microbioreactors. This means scientists can then rapidly test up to 24 cell-specific culture parameters in parallel including stirring, media formulation or feed strategies to determine the optimum conditions for cell attachment, growth rate and vaccine titre, for example.


The new method, which has been developed using Vero adherent cells attached to Cytodex 1 microcarriers,


allows a 20% media exchange to be performed at any time of the day or night on 24 ambr vessels in approximately 4h. Since the method allows even microcarrier distribution, scientists can then study up to 24 different parameters in parallel, simultaneously. This reduces reliance on spinner flasks and bench top bioreactors, saving vaccine and cell therapy manufacturers many months of scale-up process development work.


28438pr@reply-direct.com Streamline Your DNA/RNA Clean up with PowerClean Pro


The UK release of Mo Bio’s PowerClean Pro DNA and PowerClean Pro RNA cuts the number of steps needed to remove contaminants from DNA/RNA samples while minimising any chance of sample loss. The new clean- up kits, distributed exclusively by Cambio, are the latest in a range of highly effective clean-up kits produced by California-based biotech company, Mo Bio.


Clean-up kits take dirty nucleic acids and remove contaminants that could interfere with downstream processing. Enzyme-dependent applications like restriction digests, ligations, PCR amplification and sequencing all require squeaky clean DNA and RNA in order to get the best (or sometimes any) results.


Cambio already supplies a range of kits from Mo Bio designed to remove proteins, primers, dNTPs and salts often left over after an enzymatic reaction, such as PCR amplification. But if your dirty DNA/RNA sample contains contaminants other than these, you will need to bring out the ‘big guns’.


This is where PowerClean Pro DNA and


PowerClean Pro RNA come in. The new PowerClean Pro kits require fewer steps than Mo Bio’s original PowerClean DNA clean-up kit, making it less likely that you’ll lose any sample. The new post-extraction kits contain Mo Bio’s patented Inhibitor Removal Technology (IRT) for removing difficult substances that come from sources like plants, soil, faeces or blood. Common examples of contaminants, along with typical sources, include: humic acids (soil, compost); polysaccharides (plants, undigested contents in the rumen or caecum, faeces, drugs); polyphenols (plants); melanin (hair, skin, eye tissue); bile salts (faeces); haem, EDTA (blood).


If you are trying to remove something not listed here and you are unsure if a PowerClean Pro kit will work, why not try one of the new PowerClean Pro kit samples?


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