86 MICROSCOPY
In vivo imaging: reaching new depths
A high-speed, high- precision, deep- imaging multiphoton system presents new possibilities in deep, in vivo imaging. By Bülent Peker.
M
Fig. 1. Neuronal plasticity is visualised 2mm deep within the brain with the FVMPE-RS. z-stacking of neuronal tissue is cleared with the Scaleview reagent.
ultiphoton excitation microscopy combines laser-scanning
microscopy with multiphoton fluorescence to create high- resolution, three-dimensional images.
It can be used to probe deep within living tissues without damaging the sample, presenting a powerful tool within life science research, thereby achieving fundamental insights into the intricate and complex workings of biological systems, in vivo.
Olympus has responded to the need for a high-performance
in vivo imaging system with the FluoView FVMPE-RS. Dedicated to multiphoton microscopy, the system delivers rapid and accurate performance, enabling sharp image acquisition and measurement deep within the sample, even when working under the most demanding conditions.
Te system is ideal for a host of advanced imaging applications, from multicolour in vivo imaging to combined optogenetics (a technique using light to selectively activate ion channels in living specimens) and electrophysiology studies. Its
flexible and modular design also offers adaptability for researchers designing their own custom-built systems.
Te FVMPE-RS achieves exact timing of multiple imaging and stimulation protocols, as well as extended and complex time-lapse experiments. Furthermore, through advanced programming functions, sophisticated multi-position imaging and optogenetic stimulation protocols can now be effortlessly accomplished, unravelling the mysteries of brain function.
It has the ability to capture 438 fps at a resolution of 512x32 and at field number 18. Researchers can now track in great detail the most rapid processes taking place within the cell, tissue or organism – from the transport of mitochondria through neurones to blood flow analysis, or even Ca2+ signalling – all in real time.
Observing larger areas is critical for many of these functional imaging studies, and the system also captures full-frame, 512x512 images at 30 fps without compromising the field of view. Tis is made possible by the hybrid scanning unit, which builds on the capabilities of the high-speed resonant scanner.
A galvanometric scanner is also available for maximising the signal to noise ratio during deep imaging. With the ability to seamlessly switch between the two, rapid and smooth imaging with excellent definition is now realised.
An additional galvanometric scanner module is also available for simultaneous 3D stimulation and image acquisition in the
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