12 ANALYTICAL AND LABORATORY EQUIPMENT
SECTION NAME 3
Clariostar microplate reader. Designed for versatility and high performance, the new reader includes the triple technology of advanced LVF monochromators, spectrometer, and filters. Tis triple technology feature ensures that there is no compromise on sensitivity or flexibility, while high performance in all detection modes makes Clariostar ideal for assay development, too.
Te company claims the new solution is the most sensitive and flexible form of this technology currently available in a microplate reader.
LVF monochromators use linear variable filters to separate light into distinct wavelengths, unlike conventional monochromators that use metal gratings. With continuously adjustable bandwidths up to 100 nm (four times larger than conventional monochromators) and significantly increased performance, LVF monochromators have filter-like sensitivity and flexibility for fluorescence and luminescence assays.
Tey can be used in any common fluorescence or luminescence assay, but are also suited for more complex applications. Scanning the spectra of new or unusual fluorophores, where the excitation and emission wavelengths are unknown, or scanning fluorophores in non-standard buffer conditions where the pH may affect the peak maxima, is easily done. In addition, multiplexing assays with fluorophores or lumiphores of similar or overlapping spectra benefit greatly from definable bandwidths of 8 to 100 nms.
Tis allows the user to define ‘filters’ that make the assay workable. Wavelength and bandwidth can be the difference between an ordinary and an excellent assay, notes the company.
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With practical functionality, user-friendly software and affordable modularity for key user demands, BioTek has launched its Synergy HTX multi-mode microplate reader.
Tis new system is offered as an entry-level multi-mode reader to automate UV-Vis absorbance, fluorescence, luminescence and AlphaScreen/ AlphaLISA assays in 6- to 384- well microplates in 2µL samples with BioTek’s Take3 micro- volume plates.
Te novel dual-optics design results in superior filter based top and bottom fluorescence, luminescence and monochromator-based absorbance measurements for maximum applications versatility. Te company says that Synergy HTX’s modularity allows upgrading over time while its powerful Gen5 software controls all functionality while providing data analysis for many applications within one powerful yet intuitive package.
Synergy HTX has linear and orbital shaking and four-zone incubation to 50°C with condensation control to minimise plate lid condensation. Tese features, plus a dual reagent dispenser, make Synergy HTX an ideal platform for biochemical and cell-based applications such as nucleic acid quantification, cell growth and toxicity assays, kinetic ELISA and inject/read assays, says the company. In addition, workflows can be further automated with the BioStack microplate stacker or third-party automation.
New plate technology In terms of new plates, BrandTech Scientific has launched its new Brandplates inertGrade 96-well microplates for cultivation of suspension and stem cells. Available in a full range of well shapes and
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colours, these individually wrapped sterile plates have orange embossed alphanumeric lettering for easy identification of surface treatment and well locations.
Te company says its new microplates are especially suited for cell cultures when adhesion is not desired. Hydrogel technology optimises the surface characteristics to suppress interactions of cells or protein with the polystyrene plastic. Tis reduction of cell adhesion and protein adsorption minimises enzyme and cellular activation to aid in the inhibition of the differentiation of progenitor or stem cells and promotes the formation of spheroids.
All the new culture plates are sterilised by ethylene oxide to comply with the requirements of both USP 20 and
Ph.Eur standards, while also meeting the cytotoxic substance level requirement for DIN EN ISO 10993.
An interactive selection guide on the company’s website helps identify the ideal solution for a particular job, while a set of application notes show the different cells types that these microplates can be used with. Te company also offers a no-cost, obligation-free sample programme to show how intertGrade plates work in specific applications.
“…we’ve developed an innovative offering designed to enable scientists to obtain new insights from their research, which can accelerate the development of new therapeutics.”
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