ANALYTICAL AND LABORATORY EQUIPMENT 17
label-free, live cell analysis; automated fluorescence digital microscope for high content analysis using phenotypic assays; and multi-mode microplate reader.
A new solution Te Cytation 5 is a new cell imaging multi-mode microplate reader that is equipped to perform all the applications described for optical microscopy and cell-based assays.
Furthermore it provides equivalent performance to the six instruments listed above in one bench-top instrument. Te microscopy components are digitally based, consisting of LEDs for illumination, filter cubes, microscope objectives (2.5x – 60x) and a CCD camera.
Most microscopy components are located in the lower half of the Cytation 5, except for phase contrast illumination, which resides in the same upper space as the PMT-based optics of the conventional microplate reader.
Te design is also modular, allowing selection of microscopy and/or microplate reader optics as desired. Various sample vessels can be used with the microscopy modules, including microscope slides, a broad range in microplate densities (6- to 384-well), Petri dishes and tissue culture flasks (T-25).
Image acquisition, processing and analysis is performed by Gen5 software available with the Cytation 5. Fig. 1 illustrates the actual laboratory instrument and a cut-away impression of its modularity as part microscope/ part microplate reader.
As shown in Fig. 2, Cytation 5 is capable of capturing images using a variety of methods. Tese include fluorescence, brightfield, H&E and phase contrast, along with data from a cell-based
assay performed with the multi- mode detection function in the system.
Te images and data highlight the many practical applications of Cytation 5 that traditionally would have required using two or more of the instruments described above.
Hybrid approach for maximum flexibility Te multi-mode detection module features BioTek’s Hybrid Technology, which incorporates variable bandwidth monochromator optics and high sensitivity filter-based detection optics for excellent versatility
and performance. Temperature control to 65 °C and shaking, plus available CO2
and dual reagent injectors optimize conditions for cell- based imaging and detection.
/O2
Te fluorescence monochromators offer a variable bandwidth from 9nm to 50nm in 1nm increments for ultimate flexibility. Another key feature is laser-based excitation for AlphaScreen/AlphaLISA assays. Finally, the powerful yet easy to use Gen5 software enables efficient plate reading, image capture and analysis
For more information ✔ at
www.scientistlive.com/eurolab
Peter Banks is scientific director of BioTek Instruments, in Vermont, USA.
www.biotek.com
A B control
“In some laboratories, as many as half a dozen different instruments are used to perform the various applications associated with optical microscopy and cell-based assays.”
C
D
E
F
Fig. 2. A: Brightfield microscopy using a 2.5x microscope objective of 1,000 MCF-7 cells forming a spheroid in a hanging drop of media; B: H&E stained section of human intestinal wall mounted on a microscope slide and imaged with a 20x microscope objective as a 10x8 montage (80 separate images) which have been stitched together with Gen5 software to provide a composite image of wide field of view; C: High resolution microscopy of a PtK2 cell undergoing mitosis (metaphase as the chromosomes line up along the metaphase plate) using a 60x microscope objective (blue depicts the chromosomes; green the spindle fibres and red the cytoskeleton); D: Phase contrast image of lillium anther using a 4x microscope objective; E: Phenotypic assay in 384-well microplates to quantify the number of autophagosomes in the RFP channel (number of red spots) relative to the number of cells identified in the GFP channel using a 40x microscope objective; F: Dose response curves for the inhibition of AKT phosphorylation (Ser 493) by inhibitors of PI-3 kinase as measured by a cell-based ELISA assay.
www.scientistlive.com
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120