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ANALYTICAL AND LABORATORY EQUIPMENT 17


label-free, live cell analysis; automated fluorescence digital microscope for high content analysis using phenotypic assays; and multi-mode microplate reader.


A new solution Te Cytation 5 is a new cell imaging multi-mode microplate reader that is equipped to perform all the applications described for optical microscopy and cell-based assays.


Furthermore it provides equivalent performance to the six instruments listed above in one bench-top instrument. Te microscopy components are digitally based, consisting of LEDs for illumination, filter cubes, microscope objectives (2.5x – 60x) and a CCD camera.


Most microscopy components are located in the lower half of the Cytation 5, except for phase contrast illumination, which resides in the same upper space as the PMT-based optics of the conventional microplate reader.


Te design is also modular, allowing selection of microscopy and/or microplate reader optics as desired. Various sample vessels can be used with the microscopy modules, including microscope slides, a broad range in microplate densities (6- to 384-well), Petri dishes and tissue culture flasks (T-25).


Image acquisition, processing and analysis is performed by Gen5 software available with the Cytation 5. Fig. 1 illustrates the actual laboratory instrument and a cut-away impression of its modularity as part microscope/ part microplate reader.


As shown in Fig. 2, Cytation 5 is capable of capturing images using a variety of methods. Tese include fluorescence, brightfield, H&E and phase contrast, along with data from a cell-based


assay performed with the multi- mode detection function in the system.


Te images and data highlight the many practical applications of Cytation 5 that traditionally would have required using two or more of the instruments described above.


Hybrid approach for maximum flexibility Te multi-mode detection module features BioTek’s Hybrid Technology, which incorporates variable bandwidth monochromator optics and high sensitivity filter-based detection optics for excellent versatility


and performance. Temperature control to 65 °C and shaking, plus available CO2


and dual reagent injectors optimize conditions for cell- based imaging and detection.


/O2


Te fluorescence monochromators offer a variable bandwidth from 9nm to 50nm in 1nm increments for ultimate flexibility. Another key feature is laser-based excitation for AlphaScreen/AlphaLISA assays. Finally, the powerful yet easy to use Gen5 software enables efficient plate reading, image capture and analysis


For more information ✔ at www.scientistlive.com/eurolab


Peter Banks is scientific director of BioTek Instruments, in Vermont, USA. www.biotek.com


A B control


“In some laboratories, as many as half a dozen different instruments are used to perform the various applications associated with optical microscopy and cell-based assays.”


C


D


E


F


Fig. 2. A: Brightfield microscopy using a 2.5x microscope objective of 1,000 MCF-7 cells forming a spheroid in a hanging drop of media; B: H&E stained section of human intestinal wall mounted on a microscope slide and imaged with a 20x microscope objective as a 10x8 montage (80 separate images) which have been stitched together with Gen5 software to provide a composite image of wide field of view; C: High resolution microscopy of a PtK2 cell undergoing mitosis (metaphase as the chromosomes line up along the metaphase plate) using a 60x microscope objective (blue depicts the chromosomes; green the spindle fibres and red the cytoskeleton); D: Phase contrast image of lillium anther using a 4x microscope objective; E: Phenotypic assay in 384-well microplates to quantify the number of autophagosomes in the RFP channel (number of red spots) relative to the number of cells identified in the GFP channel using a 40x microscope objective; F: Dose response curves for the inhibition of AKT phosphorylation (Ser 493) by inhibitors of PI-3 kinase as measured by a cell-based ELISA assay.


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