108 BIOTECHNOLOGY
Fig. 3. With changes in incubator conditions, often caused by incubator doors being opened and closed, the G100 allows target concentrations to be maintained.
and check whether a local calibration is required on the incubator. Fig. 2. The G100 can confirm the CO2 , O2 indicate that there is a need for
where it can then be reviewed to check incubator performance or variations in readings over time. If the analyser reveals a frequently changing CO2
level, it may some further investigation.
Te product also has user- configurable alarms for setting
trigger levels or breaches in CO2 and O2
expected levels. , humidity and temperature levels
and temperature within one portable device, making it much easier and quicker to verify
Te G100 can monitor CO2
, O2 , relative humidity
performance and meaning fewer devices to be serviced and maintained.
For more information ✔ at
www.scientistlive.com/eurolab
Simon Sheridan is with Geotech, Leamington Spa, Warwickshire, UK.
www.geotechuk.com
Gamma H2AX pharmacodynamic assay kit announced Documented variability in DNA
Amsbio has announced its first commercially available gamma H2AX pharmacodynamic assay kit for the study of double strand DNA breaks through the detection of gamma H2AX - a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection. This new assay has been developed for anti-cancer drug screening, basic research and upcoming clinical trials providing one of many needed tools to support hypothesis- driven drug design strategies.
www.scientistlive.com
double-strand break repair among different segments of the human population may contribute to patient specific therapeutic toxicities, enabling a more personalised approach to treatment. A quantitative determination of
gamma-H2AX levels in response to treatment would help to establish patient specific dose regimens minimising the toxicity, while also maximising the efficacy of therapy.
Until now, induction of gamma-
H2AX has been determined by either Western Blots or scoring foci with Immunofluorescence. While Western Blots provide information regarding the gamma-H2AX, they are difficult to quantitate. Although image analysis software enables foci quantification, actual gamma-H2AX levels cannot be determined as there is no direct relationship between foci and gamma- H2AX levels after damage. Amsbio’s new 96-well non-
radioactive ELISA assay documents
differences in gamma H2AX levels in peripheral blood mononuclear cells, cultured cells, and tissue biopsies and is available as a complete reagent kit with chemiluminescent detection. Alexei Degretev from Tufts University in the USA, one of the first beta testers of the product, commented “This new gamma H2AX pharmacodynamic assay is a very useful tool. “
For more information, visit
www.amsbio.co.uk
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