104 BIOTECHNOLOGY
RNA sequencing: the complete sample-to-answer solution
Next generation sequencing (NGS) technology has revolutionised the way DNA-sequencing is performed worldwide. When commercial NGS platforms were first introduced, few people would imagine that this technology would become sufficiently accurate for reliable quantification of RNAs. However, combined with scientific knowledge and technical skills, NGS has become a very powerful tool for RNA quantification and discovery of novel
transcripts. Exiqon Services offers a complete
sample-to-answer solution with continuous support from initial consultation, sample RNA isolation, QC- results, to raw data and summary report. The company’s extensive experience of working with coding and non-coding RNA, including microRNA, is beneficial when exploring and characterising new RNA species, such as piRNAs or long non- coding RNAs (lncRNAs).
The full support offered by the
company, from experimental design to biological interpretation of data and recommendations for validation scheme, provides researchers worldwide with solid, reliable and accurate data. To ensure equally high data quality, Exiqon has developed a thorough quality control pipeline that includes RNA-QC, library preparation-QC, Raw sequencing data QC, as well as various other QC steps during data analysis. All steps in the process are performed using standard operating procedures (SOPs), from wet- lab sample handling to data analysis. The company offers quantitative
expression analysis via NGS in addition to the innate discovery analysis of the sequencing technology. RNA molecules are sequenced in an unbiased fashion; no previous knowledge of the RNA species present is necessary. In contrast to probe- based array and qPCR technologies, no pre-designed oligo-probes or qPCR primers are needed to perform a robust experiment.
Methods for authentication of human cell lines
The UK-based research institute Genome Analysis Centre (TGAC) will lead research into the development of bioinformatics to support the identification and characterisation of viruses through metagenomics. The Biotechnology and
Biological Sciences Research Council (BBSRC)funded research, led by TGAC’s Dr Richard Leggett, aims to develop computational algorithms that can accurately assemble viral genomes contained within metagenomic samples. These microbial samples pose a challenge to researchers as, not only do they contain numerous different viral species; it is also difficult to locate precisely which species are present. As a currently under explored area,
the research is vital to improve the ability to identify viruses. Beneficial to researchers involved in the study of animal-to-human transmitted viruses, disease diagnostics
www.scientistlive.com
living world. While the sequencing of metagenomic samples follows the same process as that for sequencing a single genome, it becomes more complex at the assembly stage where the short sequenced fragments of DNA must be correctly arranged into the genomes of multiple species. This intricacy is compounded by the currently limited bioinformatics tools available to conduct the assembly for metagenomic samples, particularly for those containing viruses. Having demonstrated the
and epidemiology, the three-year project will demonstrate the practical value of the developed bioinformatics tool by testing it against real datasets taken from species expected to host a variety of viruses, including a set of African rodent samples.
Samples are being provided by
collaborators led by Pablo Murcia at the MRC-University of Glasgow Centre for Virus Research.
Metagenomics is broadly defined as
environmental genomics, allowing the study of microbial communities of our
For more information visit
www.tgac.ac.uk or
www.bbsrc.ac.uk
possibility and feasibility of such a tool by the Institute’s previous MetaCortex project, the new
research will create the algorithms required to address this gap in metagenomic capabilities.
Exiqon performs sequencing with
the well-known Illumina sequencing- by-synthesis platform. The company’s sophisticated NGS laboratories offer a flexible set-up with small scale sequencing on MiSeq, mid-scale sequencing on NextSeq500, or high scale sequencing on the HiSeq2500 instruments.
In addition to the sequencing
service, Exiqon offers both qPCR and probe-based arrays for validation and further large-scale screening studies. For validation of microRNA sequencing data, the company applies its own highly sensitive miRCURY LNA qPCR and microarray systems. In summary, Exiqon Services
offers the complete sample-to-answer solution, from sample isolation to data interpretation and validation scheme.
For more information ✔ at
www.scientistlive.com/eurolab
Exiqon is based in Vedbaek, Denmark.
www.exiqon.com
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