80 MICROSCOPY
Fig. 2. Zeiss is working with the US Brain initiative on advancing tool development in neuroscience, including microscopy and advanced imaging technologies.
Capitalising on the flexibility of the modular Olympus IX83 automated inverted microscope platform, the FV1200 system is optimised for a diverse array of live cell imaging applications including fluorescence recovery after photobleaching (FRAP), fluorescence loss in photobleaching (FLIP), and photoactivation.
Capable of handling up to five simultaneous fluorescent
detection channels, the FV1200 also includes what Olympus describes as the industry’s first dedicated laser light stimulation scanner (SIM scanner), achieving simultaneous stimulation and imaging for real-time visualisation of rapid cell responses. Meanwhile UK company Image Solutions says that a new device from Yokogawa that it is now offering is an industry first because it uses 3D imaging to accurately measure cell clusters without the need for cell separation.
Te CQ1 confocal quantitative image cytometer can accurately
quantify the morphological features of live individual cells, clusters such as spheroids, colonies and also tissue sections without the time consuming need to separate cells or remove them from a culture dish (Fig. 1).
Unlike conventional devices, the CQ1 uses a microlens enhanced dual Nipkow disk confocal optical system to obtain accurate 3D images of cell morphological features and measure cell position. Tis allows scientists to obtain more accurate and reproducible data by maintaining the original biological functions and features of the cells in cell
T
Leica launches intuitive imaging software LAS X.”
he new Leica Application Suite X (LAS X) imaging software from Leica Microsystems spans all wide-field, confocal and super-
resolution platforms. It builds on the basic principles of the existing LAS
AF software, guiding users intuitively through image acquisition, data recording and evaluation in live cell imaging. Commenting on the new software, product
manager Petra Haas said, “Our software is designed to facilitate the users’ research – experiments are broken down step by step so that researchers can set up experiments more easily. That is the philosophy behind
www.scientistlive.com In detail, the software’s 3D and the 2D image
analysis wizards generate reproducible analysis results from multi-dimensional data sets. Users are guided through the workflow step-by-step, from applying filters, thresholding, binary image processing through to measurements and classification. Analyses between separate channels can be
combined to obtain object-specific data. For example, a binary reference mask can be applied to count the number of spots in each nucleus. Within the 2D analysis wizard, tracking experiments can be performed, and image analysis protocols can be combined with ImageJ
clusters. It is also possible to continue culture experiments for repetitive measurements in order to examine chronological changes – saving time and improving testing efficiency. A fully customised CQ1 system can also be integrated with external systems for robotic culture dish handling.
Te CQ1 is already growing in importance in areas such as regenerative medicine, cell quality evaluation for drug discovery, pharmacology, biopsy evaluation and the study of cancer and other diseases where it is essential to accurately quantify
macros. To save time, repetitive analysis protocols can be stored and applied to several experiments. Analysis results are saved with the experiment or can be exported Excel for further investigation. As well as this, the hardware-based adaptive
focus control (AFC) and software-based autofocus are integrated in LAS X. Experimental conditions such as temperature, carbon dioxide and oxygen are documented and controlled with the environmental control module. For long-term time-lapse and screening
experiments, the software-controlled micro dispenser compensates for evaporation during water immersion.
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92 |
Page 93 |
Page 94 |
Page 95 |
Page 96 |
Page 97 |
Page 98 |
Page 99 |
Page 100 |
Page 101 |
Page 102 |
Page 103 |
Page 104 |
Page 105 |
Page 106 |
Page 107 |
Page 108 |
Page 109 |
Page 110 |
Page 111 |
Page 112 |
Page 113 |
Page 114 |
Page 115 |
Page 116 |
Page 117 |
Page 118 |
Page 119 |
Page 120