30 ANALYTICAL AND LABORATORY EQUIPMENT
employed that suitably retained the polar trichothecenes. Using this column, the first compound (nivalenol) does not elute until 3.65 minutes. Additionally, the first two minutes of flow was diverted to waste minimising ion source contamination. Te separation of the mycotoxins, including α- and β-zearalanol, was achieved within 16 minutes. Te use of rapid polarity switching allowed all target analytes to be detected in a single run.
Te experiment produced several clear conclusions. Firstly, SPE extraction of the mycotoxins, including the polar trichothecenes, was successfully achieved using an EnviroClean hypercrosslinked divinylbenzene SPE cartridge. Te SPE wash step was optimised to remove matrix interferences without analyte loss. Next, separation of the mycotoxins, including baseline resolution of α- and β-zearalanol, was achieved
Fig. 2. Accuracy and precision data obtained for the SPE sample preparation method.
within 16 min on a polyaromatic Selectra DA HPLC column. APCI ionisation was selected as it provided the best overall results, including better signal response for problematic trichothecenes. Using rapid polarity switching allows all target analytes to be detected in a single run. Overall, good accuracy and precision data were
obtained. Finally, for best results, matrix-matched calibration curves and isotopically labelled internal standards are recommended.
For more information ✔ at
www.scientistlive.com/eurolab
Brian Kinsella is an application chemist at UCT, in Philadelphia, USA.
www.unitedchem.com
Getting the most out of your PCR - Results Count!
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EuroLab_Dec14_PCR-Cons_FINAL_23Oct14.indd 1 For more information ✔ at
www.engineerlive.com/asia
23/10/2014 16:42:58
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