MICROSCOPY 79
Simplifying specialised cell imaging
Eugene McCarthy reports on how vast improvements in optical resolutions and 3D imaging are allowing laboratories to simplify routine tasks and carry out real-time cell imaging.
Eugene McCarthy nous rend compte des progrès énormes dans le domaine des résolutions optiques et de l’imagerie 3D qui simplifient les tâches de routine des laboratoires et permettent l’imagerie cellulaire en temps réel.
Eugene McCarthy berichtet darüber, wie Labors dank umfangreicher Verbesserungen bei der optischen Auflösung und bei 3D-Bildverfahren Routineaufgaben verbessern können und die Zellbildgebung in Echtzeit durchgeführt werden kann.
A
Fig. 1. The CQ1 confocal quantitative image cytometer can accurately quantify the morphological features of live individual cells without the need to separate them.
novel upgrade from Olympus is allowing researchers who use
microscopy for cell and tissue imaging to obtain optical resolution of up to 120nm.
With Olympus FV-OSR software, the FluoView FV1200 confocal laser scanning microscope is transformed into a powerful system for super resolution microscopy – giving easy access to what was previously the sole domain of highly specialised microscopy systems.
Te extra detail offered by super resolution technology helps researchers reveal the subtlest mechanisms at work within cells or tissue.
Compared with standard wide- field imaging, 3D confocal laser scanning microscopy achieves vastly improved lateral and axial resolution. However, it is still limited by the diffraction limit of light. In certain settings, images can greatly benefit from enhanced contrast after post-acquisition processing. Tis is where super resolution microscopy comes into play, breaking the diffraction limit of light and enhancing cell- and tissue-imaging studies.
Te company says that this approach makes the technology far more accessible to the life science research community because of its low cost when compared with traditional super resolution microscopy.
www.scientistlive.com
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