BIOTECHNOLOGY 107
Maintaining accurate and reliable incubator readings
Ensuring that
temperature and CO2 levels are accurate within an incubator is integral for medical, IVF-clinic, research and pharmaceutical applications. Simon Sheridan reports.
I
optimum cell growth. Terefore a convenient and accurate means to check these parameters is essential.
and CO2
highest quality of on-board instruments can sometimes drift out of calibration, for example, as a result of intense cleaning and maintenance regimes at high temperatures.
While incubators themselves have a built-in system for monitoring CO2
, even the
Te Geotech G100, which measures CO2
and O2
Fig. 1. (Above) Laboratory incubators should run at consistent temperature, humidity and CO2 levels. Geotech’s portable gas analyser verifies the built-in monitoring equipment.
from 0-20% verification of incubators in
atmosphere inside incubators. It was specifically designed to monitor CO2
from 0-100%, is used to demonstrate accurate and repeatable CO2
for the readings of the
t is critical that laboratory incubators run at a consistent temperature, humidity level to maintain
medical, IVF-clinic, research and pharmaceutical applications.
With the G100 an incubator reading takes just a few seconds, enabling embryologists to quickly take readings from various incubators, all of which will have a different means of sampling the air inside an incubator, without fully opening the inner door and adversely affecting the environment.
Te G100 can confirm the CO2 O2
, humidity and temperature
levels and check whether a local calibration is required on the incubator. Monitoring can be carried out during day-to-day operation of the incubators by a lab technician or scientist.
Te G100 can be used by incubator supplier technicians during servicing, maintenance and performance evaluation visits. Monitoring allows for
,
User-defined gas checks on incubators can be performed
with the G100 to verify CO2 concentrations, and as the incubator CO2
concentration
becomes consistent these gas checks may be performed less frequently.
Te data from the G100 can be recorded on spreadsheet, data logged or exported to Geotech’s optional download software –
www.scientistlive.com
validation of all incubators to ensure they are at an optimum for culture growth.
Te system allows for fine-
As incubator doors are frequently opened and closed, CO2
concentration levels vary.
and temperature within the chamber.
tuning and ensures target CO2 concentrations are maintained. Tis also avoids any significant variation in O2
, relative humidity
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