LIFESTYLE COSMETICS
Balancing epigenetics for skin wellbeing
Angela Roca PhD,Miguel Aso-Pérez PhD, Blanca Martinez-Teipel PhD, Jordi Bosch – Provital, Spain
Age is no longer a taboo. Health and wellbeing have become the most important aspects over the course of a lifetime. Focusing on lifestyle and having a positive attitude towards life are the new demands of the more mature generations. Actually, over the past decade, a growing number of studies have revealed that progressive changes to epigenetic information have a huge influence on the ageing process. Lifestyle habits, diet, pollution and other environmental factors can influence our life span by changing the epigenetic information.1 Epigenetics refers to heritable and
reversible changes in gene expression that do not involve changes to the underlying DNA sequence. Among the epigenetic modulators are microRNAs (miRNAs), a class of short, endogenous, single-stranded, noncoding RNA molecules. By binding a specific sequence of the target mRNAs, they can regulate the expression of multiple genes at the post-transcriptional level through degradation or translational inhibition of the targeted transcripts, thereby decreasing protein synthesis.2
A single miRNA
can target up to several hundred mRNAs, and one gene is targeted by many different miRNAs. Therefore, by modulating miRNAs levels we can significantly harmonise gene expression regulatory networks. Numerous miRNAs have been described with an important regulatory role in the different hallmarks and processes of ageing.3 More precisely in skin, miRNAs have specific roles such as regulation of cell senescence in both keratinocytes and fibroblasts,4
miRNAs
100 90 80 70 60 50 40 30 20 10 0
are involved in the stress response in the extracellular matrix with the loss of proteins such as collagen and elastin fibres5
and in
skin diseases such as atopic dermatitis and psoriasis.6
Therefore, miRNAs intervene in many
of the pathways behind skin ageing, modulating gene expression and changing the aspect of aged skin, increasing its fragility and wrinkles, leading to drier skin. Healthy ageing is not only about limiting or
reversing the biological/genetic factors involved in the ageing process, but there are also other important components to achieve successful ageing; the maintaining of high cognitive and physical function together with engagement with life. Moreover, there is a strong psychological
component to achieve a positive lifestyle when we grow older, where emotions play an outstanding role. Several studies have shown the positive impact of cosmetic products generating good feelings or positive emotions. In response to this modern mindset and
positive approach to ageing, Provital launched Wonderage™, a well-ageing natural active ingredient derived from Monk Fruit, that balances the epigenetics of skin to enhance natural radiance of mature women and impact on their emotional wellbeing. Two types of studies were performed with
the cosmetic ingredient based on the monk fruit (Momordica grosvenorii). The first analysed the ageing effects both in vitro and in vivo, and the second one explored the emotional benefits of the ingredient’s use in mature women.
ABSTRACT
Rather than looking younger, the more mature generations want to feel at one with their age and show off the very best version of themselves. Since more than 90% of decisions are made subconsciously, Provital - with its everlasting commitment to innovation and technological progress in the interests of caring for people - used Artificial Intelligence to demonstrate the emotional impact that its active ingredient Wonderage™ had on the subconscious of 47 volunteers, providing a holistic approach to an ingredient with a physical improvement on skin luminosity, hydration and density achieved by its effect on the epigenetic signature and on the endogenous hyaluronic acid production. Because overall wellbeing is seen
as integrative beauty that embraces both the physical aspect and emotional health. Because happiness is the key to
beauty.
In vitro methods MiRNA profiling analysis by microarray in aged dermal fibroblasts Normal human dermal fibroblasts were obtained from a 66-year-old woman and were cultured in the absence or presence of Monk fruit active at a concentration of 0.93%. After 24 h, fibroblasts were lysed and total RNA including miRNA was extracted with the miRNeasy Mini Kit. Total RNA was analysed using NanoDrop and Agilent 2100 Bioanalyser to assess the RNA integrity and the presence of miRNA in the samples. For microarray analysis, 250 ng of total RNA
Figure 1: miRNAs differential expression in aged fibroblasts.
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were labeled using a 3D-Gene miRNA labelling kit from Toray Industries. Labelled RNA was hybridised to 3D-Gene human miRNA Oligo chips version 21 that allow us to detect about 2000 human miRNA from miRBase release 21 (
www.miRBase.org). Finally, fluorescence intensities were determined using a 3D-Gene Scanner and analysed using the R biostatistical software. Only the miRNAs that passed the normalisation filter were considered and the significance threshold was set at a fold change (FC) >2 or <0.5.
June 2021 PERSONAL CARE
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miR-378a-3p miR-422a miR-125b-3p miR-4299-5p miR-4462-5p miR-6830-5p miR-5195-5p miR-6731-5p
Reduced expression (%)
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