SKIN PROTECTION STUDY OF COLLAGENS OF TYPE 1 Collagen (pg/ml) STUDY OF COLLAGENS OF TYPE 3 Collagen III (pg/ml)
49
+15%
+21% Control H2 O2
Vit C (1%) + H2
O2
IR [CC+AA] (0.5%)+ H2
O2
+28%
IR [CC+AA] (1%)+ H2
O2
+35%
IR [CC+AA] (2.5%)+ H2
O2
INCREASE OF COLLAGENS OF TYPE 1 AND TYPE 3 At concentrations of 0.5%, 1% and 2.5%, after exposure to hydrogen peroxide, Increase of collagens of type 1 respectively of 21%, 28% and 35% and increase of collagens of type 3 respectively of 29%, 37% and 44%
Figure 4: Studies of collagens type 1 and 3.
Study of the constituents of the extracellular matrice Naolys studied two types of essential components of the extracellular matrice: polysaccharides (proteoglycans and glycosaminoglycans-GAGs), and collagens of type 1 and collagens of type 3. Polysaccharides enable cell-matrix interactions, cell movement and control of the cellular environment in terms of hydration and ionic balance. Naolys studied the synthesis of three
types of proteoglycans. Proteoglycans are the result of a combination of a protein and GAGs. As they are made of long O-glycosylated chains, they act as “water traps”. They have moisturising, buffering, liaison and resistance properties. GAGs are large acidic chains that have a substantial capacity to attract water. There are many GAGs, hyaluronic acid is an example.
Study of perimembran, transmembran and matricial proteoglycans To determine the existence of an increase of proteoglycans, at concentrations of 0.5%, 1% and 2.5%, after exposure to hydrogen peroxide,
STUDY OF THE PROLIFERATION OF KERATINOCYTES ON RECONSTRUCTED EPIDERMIS
Number of labelled cells (ki-67)
an increase was demonstrated of perimembran proteoglycans respectively of 25%, 29% and 37%; increase of transmembran proteoglycans of 24%, 31% and 33%; increase of matricial proteoglycans of 26%, 34% and 41%.
Study of glycosaminoglycans At concentrations of 0.5%, 1% and 2.5%, after exposure to hydrogen peroxide, an increase of the neosynthesis of glycosaminoglycans respectively of 28%, 36% and 44% (VS vitamin C only: +23%) was shown.
Study of collagens of type 1 and collagens of type 3 Collagens provide resistance to stretching, regulate cell adhesion (the process by which cells attach to the ECM), support chemotaxis and cellular migration, and help to direct tissue growth. Collagens are the most abundant fibrous proteins in the interstitial ECM and constitute the ECM’s main structural component, in particular the fibrillar collagens: type I collagen represents 60% to 80% of collagens in the dermis, type III collagen from 15% to 25%. Type I, III, and V fibrillar collagen self-assemble in thicker fibres that form a three-
dimensional network in the entire thickness of the dermis. They give the skin its resilience and are essential to its tissue integrity.
Results At concentrations of 0.5%, 1% and 2.5%, after exposure to hydrogen peroxide, increase of collagens of type 1 respectively of 21%, 28% and 35% and increase of collagens of type 3 respectively of 29%, 37% and 44% was observed.
Improving skin density by regenerating epidermis The papillary dermis (uppermost layer of the dermis) is the intermediary layer rich in nerve endings and in permanent symbiosis with the epidermis, from which it is separated by the dermal-epidermal junction. If the dermis is functioning optimally, then the cells in the basal layer of the epidermis are better irrigated with nutrients, and renew themselves more efficiently and more quickly. This is very important because if this cellular
renewal is less efficient, it will be reflected in the skin’s surface with obvious signs of ageing: withering of the skin due to the reduction in the proliferation of epidermal cells, poor healing in the event of injury, hair loss, etc. To monitor the condition of the epidermis,
Naolys studied its renewal via the nuclear protein Antigen Ki-67, a marker for cell proliferation.
+17% +21% Control Vit C (1%) +20% +23% +27%
Results To assess the increase of Ki-67, at concentrations of 0.5%, 1% and 2.5%, stimulation of the proliferation of keratinocytes of the basasl layer, respectively of 20%, 23% and 27% was observed.
INCREASE OF Ki-67 At concentrations of 0.5%, 1% and 2.5%, sitmulation of the proliferation of keratinocytes of the basasl layer, respectively of 20%, 23% and 27%.
IR [CC+AA] (0.5%)+ H2
O2 Figure 5: Study of proliferation of keratinocytes.
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IR [CC+AA] (1%)+ H2
O2
IR [CC+AA] (2.5%)+ H2
O2
Conclusion Thanks to its targeted action in the heart of the dermis, Inner Renewal [CC+AA], from Naolys, enables the skin to retain a younger, less wrinkled appearance. Using active cells as a complete delivery
system, Naolys Cornflower cells deliver vitamin C to help key skin cells maintain their functions for longer, focusing on keeping the skin naturally firm.
PC June 2021 PERSONAL CARE Control H2 O2
+20%
+29%
Vit C (1%) + H2
O2
IR [CC+AA] (0.5%)+ H2
O2
+37%
IR [CC+AA] (1%)+ H2
O2
+44%
IR [CC+AA] (2.5%)+ H2
O2
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