SKIN CARE Control ■ 0.1% Goji stem cell extract ■
1000 800 600 400 200 0
Control CRCT1 LCE2B LOR
Figure 3: Increased expression of genes important for skin barrier formation
in phosphate buffered saline (PBS) solution and immediately frozen at -80°C. Total RNA was extracted from the cells and the expression of extracellular matrix genes was then assessed with RT-qPCR using the LightCycler System (Roche).
Clinical anti-aging study In a randomized, placebo-controlled clinical study, 23 Caucasian women aged between 41 and 69 years (mean age: 56 years) who displayed signs of photoaging applied either a cream with 0.4% goji stem cell active (INCI: Lycium Barbarum Callus Culture Extract (and) Isomalt (and) Lecithin (and) Aqua / Water) on one half of the face and one forearm and the corresponding placebo on the other half of the face and the other forearm, twice daily for a period of 56 days. The wrinkle depth was measured using PRIMOS lite (Canfield). The density of the
A BEFORE AFTER 28 DAYS
Control ■ 0.1% Goji stem cell extract ■ 2
1.5 1 100 0.5 0
Total protein quantification
Enzyme activity quantification
Figure 4: Increased exosome production in mesenchymal stem cells
skin (epidermis + dermis) was determined by ultrasonic measurements in triplicates using DermaScan C (Cortex) which counts the number of low-echogenic pixels corresponding to low- density areas on a 2-D portion of epidermis and dermis. The lower the number of dark pixels the higher the skin density.
Clinical face contour study In a randomized, placebo-controlled clinical study, sixty-seven Caucasian women aged between 39 and 70 years (average age 57 years) with sagging facial skin were split into two groups. One group applied a cream with 0.4% goji stem cell active and the other group applied the corresponding placebo cream on the entire face and neck twice daily for 28 days. The facial sagging and oval face shape was
determined by measuring the size of the neck/ submandibular triangle area by image analysis of the pictures of the volunteers’ faces that were
B BEFORE
Figure 5: Improved cell-cell communication assessed by gene expression analysis in fibroblasts treated with conditioned medium from mesenchymal stem cells (MSCs)
taken with Visioface 1000 D (Courage + Khazaka). For the image analysis, a straight line was drawn vertically through the middle of the chin. On either side of the face, vertical parallel
lines were drawn to intersect at the start of the jawline/sagging skin. The points of the jawline intersection were then connected with the middle of the chin. The area below the drawn V lines was determined. A reduction of this area would suggest a reduction in facial sagging and an improved V-shape of the face.
Clinical breast lifting study The effect of goji stem cell active on breast sagging was tested in a randomized, placebo- controlled clinical study with 44 women aged between 18 and 44 years (mean age: 29.4 years) with breast sizes 80B, 85A, 90A, and 90B. The women were split into two groups of 22
AFTER 56 DAYS 0 * 200
45
Conditioned medium from untreated MSCs ■ Condition medium from MSCs treated ■
with 1% Goji stem cell extract *p<0.05 versus control 300
BEFORE
AFTER 56 DAYS
Figure 6: Improved skin density and wrinkle appearance. A: Subepidermal low-echogenic bands (SLEB) were reduced after 28 days of treatment with goji stem cell active. B: Wrinkles appearance in the crow’s feet area was visibly improved after 56 days of treatment with goji stem cell active
www.personalcaremagazine.com January 2025 PERSONAL CARE
Gene expression changes compared to control (=100) in %
Increase in exosome levels normalised to control (=1)
Change in gene expression compared to control (=100) in %
Control COL3A1
COL16A1 Elastin PLOD1 LOX
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