60 Assay Kits, Biomarkers and Antibodies Supporting Oxidative Stress Research
It is widely recognised free radicals play a key role in physiological processes including protein phosphorylation as well as activation of transcriptional factors and immunity, but they need to be kept at a low level.
Changes in environmental factors, diet and lifestyle are known to contribute signifi cantly to an increase in free radical production in the body. When free radicals are not kept in balance, they trigger a large chain of oxidation reactions putting the body under oxidative stress which can lead to damage to fatty tissue, DNA, and proteins. Oxidative stress damage over time is linked to diseases including cancer, diabetes, atherosclerosis, cardiovascular degeneration, infl ammatory conditions, neurodegeneration, and premature aging.
AMSBIO has expanded its range of antibodies, biomarkers and assay kits related to free radicals (ROS and RNS), non-radical reactive derivatives (or oxidants) and antioxidants for researchers investigating the mechanism of oxidative stress.
Partnering with leading international research organisations, AMSBIO is today able to offer an unmatched range of top-quality antibodies and cutting-edge assay kits for oxidative modifi cation, redox modulators, and infl ammatory biomarkers.
This extensive range includes simple, accurate oxidative stress kits to analyse biomarkers of DNA, lipid, and protein oxidative modifi cation in biological samples, highly sensitivity assays for 8-oxo dG (8-OHDG), nitrotyrosine, protein carbonyl, malondialdehyde, 8-isoprostane, and superoxide dismutase. The new kits combine high sensitivity, convenient 96-well format colorimetric assay format and offer the peace- of-mind of being well cited in scientifi c literature. For labs tasked with running many samples, most AMSBIO oxidative stress assay kits are supplied with multiple plates to increase productivity. All AMSBIO oxidative stress assay kits are supplied ready-to-use and produce highly reproducible results.
In addition, AMSBIO has expanded its range of antibodies for oxidative stress research, to now include targets such as nitric oxide synthase, endothelial nitric oxide synthase, perioredoxin, RAGE, sodium azide and thioredoxin.
More information online:
ilmt.co/PL/1lMV 58281pr@reply-direct.com
Fully Automated Lab-scale Bioreactors
Froilabo have announced the launch of a new range of fully automated lab-scale bioreactors. Expanding their portfolio of temperature-controlled laboratory equipment, the bioreactors offer a great range of application possibilities suited to academia, research, process development and optimisation, scale-up and production.
The vessels feature a novel magnetic mixer and non-welded lid and are ideal for bioprocesses which require high-grade aseptic conditions. The design of the mixer enables numerous customisation options: the impeller placement, amount and type can be adjusted to best suit the application and may be fi xed or detachable, as required. The autoclavable single walled borosilicate glass vessels are available in three different volumes (1, 5 and 15 litre) to make process scaling simple.
The bioprocess controller is simple and intuitive, enabling a high standard of process supervision and control. Featuring a 15” touch screen, the controller has 3 levels of access which are password protected. A specialised version of SCADA is available which is compliant with the requirements of 21 CFR Part 11.
Antonin Duval, CEO, said: “We have listened to our customers and are delighted to introduce bioreactors to our growing portfolio. As always, we seek to offer the highest quality instruments to our customers in conjunction with outstanding customer support and service.”
More information online:
ilmt.co/PL/Y0M1 58989pr@reply-direct.com Streamlining the Study of Protein-DNA Interactions in Severe Diseases
Researchers at the University of Ostrava, Czech Republic, are using a 12 channel 50 μl VOYAGER adjustable tip spacing pipette and ECO Rack GripTip pipette tips from Integra Biosciences to study the role of protein-DNA interactions in the progression of severe diseases, such as cancers and neurodegeneration. These tools have enabled the team to cut pipetting times by 75%, freeing up staff to perform other vital tasks and increasing the lab’s sample throughput.
A team within the Department of Biology uses the VOYAGER with GripTips to isolate DNA, RNA and proteins from recombinant bacteria and plants, sometimes processing more than 100 biological samples per day. The group then studies the noncanonical nucleic acids structures - such as four- stranded G-quadruplexes, left-handed Z-DNA, and cruciform DNA - and how these unusual conformations interact with proteins, providing valuable insights into the development of numerous serious conditions.
Dr Martin Bartas, a researcher in the department, explained: “We are using the 12 channel VOYAGER pipette to perform DNA and protein extractions, making it possible to carry out quick and easy transfers between 96 well qPCR or microtitration plates and 1.5 ml microtubes. We also use the pipette to rapidly fi ll qPCR plates with master mix preparations, streamlining the whole pipetting process.”
Transferring samples between different labware formats was previously very challenging for the team, but the VOYAGER pipette has allowed them to rapidly adjust tip spacings and pipette a single 96 well qPCR plate in less than 15 minutes, compared to nearly an hour with a single channel pipette. “We are now able to pipette signifi cantly more qPCR plates per hour using the VOYAGER, because it saves so much time. On top of this, we are also seeing smaller deviations between technical replicates, giving us peace of mind and reassurance that our results are extremely accurate and reliable,” Martin concluded.
More information online:
ilmt.co/PL/eV4B 59004pr@reply-direct.com
Hydrogel Reagent Kits for Agarose Encapsulation and 3D Cell Culture Research
Dolomite Bio’s hydrogel focused reagent kits enable high-throughput encapsulation of cells in hydrogel scaffolds. The nadAROSE and nadia3D kits address the needs of researchers exploring both agarose encapsulation and collagen-based hydrogel projects for the 3D cell culture space.
Current methods for constructing hydrogel scaffolds involve costly, time consuming, ‘home brew’ batch methods, with few commercially available solutions. For this reason, Dolomite Bio is launching the nadAROSE kit for the encapsulation of cells in agarose and the nadia3D kit for 3D cell culture research. Both kits are complete, standardised, and reliable solutions, containing all the single use, prefabricated consumables needed for scaffold production on the Nadia Instrument.
Due to the fl exible nature of the Nadia Instrument, researchers can perform high throughput encapsulation of up to 1 million cells in one run, with a choice of running 1,2,4 or 8 lanes in parallel, in under 30 minutes. The fully automated Nadia Instrument guides users through all relevant steps of the experiment via an easy-to-use touchscreen interface. With single use chips and precise temperature control, the Nadia instrument is ideally suited for your hydrogel research projects. Additionally, researchers will benefi t from the wide sample compatibility, opening up research areas involving large cells of over 50 µm in diameter, such as cardiomyocytes.
Most importantly, both kits can offer control over mono-clonality, giving researchers access to a variety of downstream workfl ows, such as, cell secretion studies, cell-cell interactions, microbial cell culture. Furthermore, the nadAROSE kit is compatible with cytometric analyses. The nadia3D kit opens up downstream workfl ows in: the development of novel culture techniques for fastidious cell lines, disease modelling, identifi cation of novel drug targets and pharmaceutical screening.
More information online:
ilmt.co/PL/O2lm 58306pr@reply-direct.com
INTERNATIONAL LABMATE - NOVEMBER 2022
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