Chromatography 19 Failsafe Monitor to Shut Off your HPLC if a Solvent Reservoir Runs Dry
The Solvent Line Monitor from Testa Analytical is a true plug-and-play addition to any HPLC system designed to detect when one or more of its pump solvent reservoirs are empty and automatically shut-off the system.
Modern chromatography systems often include automation enabling them to achieve a high sample throughput which was unthinkable just a few years ago. This has led to many organisations reducing the number of lab personnel trained to run HPLC systems. Today, analytical laboratory staff typically have to monitor and manage several different chromatography systems at the same time. In such a scenario, overlooking a small detail such as making sure suffi cient solvent is available for all runs planned, can easily occur.
Running out of solvent when running an HPLC or any other liquid chromatography system is a problem most separation scientists will have encountered. Your chromatograph running out of solvent can lead to loss of data, need to re-prime the pump, system downtime and often necessitates re-validation to make sure the obtained results are within expected limits. The Solvent Line Monitor is designed to eliminate this problem, without any user input or need for regular resetting. The Solvent Line Monitor runs independently and is fully compatible with almost any liquid chromatograph.
The Solvent Line Monitor is purpose designed to continuously monitor the fi ll status of tubing carrying solvent to up to two liquid chromatography pumps. It can alert the operator, or even automatically shut down the operation of an HPLC System, if no solvent is present in the tubing. The device operates fully independently from any other component and requires no special parameter setting.
Using the compact and affordable Solvent Line Monitor is a straightforward process. The device is set-up and controlled using an intuitive PC-based app that provides facility for different actions once an empty reservoir alarm status is detected, for example a simple beep or a stop signal.
More information online:
ilmt.co/PL/6lRL 58934pr@reply-direct.com
PEEK and Stainless-Steel Sample Loops
Inject with accuracy and reproducibility with the economical PEEK and Stainless-Steel sample loops from MicroSolv. PEEK sample loops available with volumes up to 20μl are manufactured of tubing with the closest tolerance (OD and ID: +/-0.025 mm) available; the Super-T™ PEEK tube, which guarantees the smallest volume deviation possible thus providing better results.
Stainless Steel sample loops use tubing that is welded and annealed. The tubing is tested according to EN 10204/3.1B and is cut using EDC (electronic discharge) techniques to achieve square and burr-free end for zero-dead volume connections. Tolerances for SS tubing and capillaries are +/- 0.025mm for both OD and ID. Many different volumes may be available upon request.
More information online:
ilmt.co/PL/rA52 58885pr@reply-direct.com
ADVERTORIAL Technical Note: Strategies to Improve Your HIC Analysis
Hydrophobic interaction chromatography (HIC) is a separation mode used for the analysis of biomolecules such as antibody-drug-conjugates (ADCs), monoclonal antibodies (mAbs) or proteins in general. Analytes are separated based on the differences in their surface hydrophobicity similar to reversed phase (RP) chromatography. However, a big plus is that analytes maintain their original structure and therefore functionality, making HIC a useful separation mode for intact protein analysis or protein purification.
HIC utilises reversible interactions that occur between the analyte and hydrophobic stationary phase ligands attached to the particle surface by applying an inverse salt gradient. A high salt concentration in the beginning of the gradient enhances the hydrophobic interactions between the target molecules and the stationary phase resulting in their retention. By decreasing the salt concentration, the strength of the interaction weakens and the analytes elute.
Improvements of a HIC separation can be achieved by varying the temperature (here: 3 mAbs at 15, 25 and 40°C).
In this new Technical Note by YMC you will learn insights about:
• How to increase the retention by using elevated temperature and high initial salt concentrations
• The influence of salt concentration on sample precipitation
• Improving hydrophobic interactions by selecting the right pH
• How shallower gradients provide higher resolution
• The infl uence of organic modifi ers on peak shape and retention
If you want to know more about the resolving power of non-porous HIC columns for intact proteins, check out this Technical Note.
More information online:
ilmt.co/PL/6l2Q and
ilmt.co/PL/L293 58796pr@reply-direct.com
Experts in Reproducibility
• Robust Bio-RP (U)HPLC Extremely inert particles for sharp peaks of proteins/peptides, oligonucleotides or mAbs.
• High Recovery IEX Low adsorption and excellent resolution in proteins,
mAbs and oligonucleotides analyses.
• Highly Efficient HIC & SEC Different selectivities for fast and reliable analysis.
of proteins, mAbs and ADCs.
See our new website:
www.ymc.eu – Latest news and detailed support YMC Europe GmbH · Email
support-lca@ymc.de · Phone +49(0)2064427-0
WWW.LABMATE-ONLINE.COM
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