18 New UHPLC Tool for Superior Biopolymer Analysis


Shimadzu has introduced the new ‘Nexera XS inert’, a biocompatible, bioinert Ultra-High Performance Liquid Chromatography (UHPLC) system. It offers the same superior reliability, robustness and expandability as other Nexera series UHPLC systems. It is particularly well-suited to analysing biopolymers such as antibodies, peptides or nucleic acids. The Nexera XS inert is also equipped with additional new and advanced product features, such as bioinert column switching, finger- tightened UHPLC connectors and a real-time pH monitor.


Determination of Oxygenates and BTEX in Beverage Grade Carbon Dioxide by GC


The following gas chromatography (GC) method was developed for the simultaneous analysis of ppb levels of the oxygenates acetaldehyde, dimethyl ether (DME) and benzene, toluene, ethylene and xylene (BTEX) in beverage grade carbon dioxide by installing a second column to separate acetaldehyde and DME from the carbon dioxide and the BTEX. A four port switching valve was used for column selection.


This GC method was performed on a process analyser configured with a photoionisation


detector which has a detection limit for BTEX with no preconcentration at < 0.5 ppb; acetaldehyde at <50 ppb and DME at < 20 ppb. The analysis was automated via a programmable logic controller (PLC) that controls the analyser (start, calibrate, send/print report). The embedded software operating system is Windows®


The Nexera XS inert UHPLC features a metal-free sample flow path that prevents unwanted interaction with biopolymers prone to adsorption onto exposed metal sites. By doing this, the Nexera XS inert achieves reduced sample loss and excellent peak shape for reproducibly high sensitivity and high-quality, reliable data. In addition, all wetted surfaces are prepared from corrosion-resistant materials, rendering them stable against mobile phase solvents containing high concentrations of salts or acids.


The Nexera XS inert system consists of stainless-steel-casing-reinforced PEEK tubing (polyether ether ketone), a high-performance engineered polymer with mechanical and chemical resistance properties. It ensures a system pressure tolerance of > 100 MPa while maintaining a bioinert flow path. The ceramic injection needle offers metal-free injections and minimised carryover.


The system’s 105 MPa pressure resistance enables the use of long separation columns packed with small-sized particles to achieve ultra-high efficiency in a wide variety of applications. The newly developed PEEK-lined tubing comes with tool-free, finger-tightened fittings. These permit reliably connected columns to withstand a pressure of up to 105 MPa without creating any dead volume or risking damage by overtightening. All valves used in the Nexera XS inert system are designed to inhibit metal adsorption.


Analysing biopolymers such as proteins, peptides and nucleic acids requires columns with different separation modesThe Shim-pack series of columns is ideal for the analysis of a wide variety of biopolymers. In addition, adsorption of target analytes in the sample vial can greatly affect the analysis and requires special attention. Shimadzu offers the TORAST-H Bio Vial, a low-adsorption PP vial, e.g., for peptide drug research, and TORAST-H low- adsorption glass vials as a general-purpose option.


10 which utilises PeakWorks, a chromatography integration system


for peak identification and quantification. Data was generated that yielded the following results: <100 ppb (acetaldehyde), <50 ppb (dimethyl ether), and 5 ppb BTEX for a direct injection with no concentration of the sample. The analysis time for this multicomponent analysis was < 10 minutes. This system can be remotely operated.


Contact PID Analyzers to request a quotation or learn more. More information online: ilmt.co/PL/VORd


57924pr@reply-direct.com Versatile System Removes FID Response Variability


Using a series of post-column reactions, the Polyarc System converts all analytes to methane for detection with GC-FID. This provides the FID a universal and uniform carbon response with a 7-order of magnitude linear range from <100 ppb to 100% for nearly all organic compounds. The Polyarc System improves laboratory workflows and efficiencies by removing the variable response of the FID. It gives scientists the capability to determine purity, quantify unknowns, qualify products,


investigate, improve development and more in a simple, fast and more economical way that is transforming the industry.


The Polyarc system is the first commercial technology of its kind. The device uses proprietary technologies including novel catalysts, a specially designed 3D-printed micro reactor, and fast seamless integration into existing equipment. The optimised internal design and catalyst microstructure maintains separation performance and peak shape. Chromatographers can expect the same resolution with conversion rates greater than 99.9% to methane. The careful selection and robust catalyst design enables the analysis of a wide variety of compounds including a range of heteroatoms, compounds of various sizes, and samples containing common poisons (e.g., S).


A truly universal carbon response transforms how GC-FID analysis can be done. It provides increased sensitivity for highly substituted organic, which historically have not been compatible with FID. Compounds such as formaldehyde, formic acid, carbonyl sulphide, and formamide that have previously been difficult to detect trace levels with GC-FID, can now be seemly analysed. Another benefit is reduced need for time-consuming calibrations with difficult to acquire standards. Removing numerous calibrations from your analysis will not only streamline laboratory work, but also reduce chemical inventory. The only remaining variability in the GC system is the sample introduction, which is why the Polyarc System is frequently used for method development. The Polyarc System has had widespread acceptance across multiple industries by leading GC experts.


Contact JSB today and learn how you can change your way of conducting GC-FID. More information online: ilmt.co/PL/Ey9R


57830pr@reply-direct.com


More information online: ilmt.co/PL/nR90 58295pr@reply-direct.com


How Multi-Column Chromatography Improves Downstream Processing and Saves Money


The traditional capture chromatography method for processing (single-column approach) requires a lot of time, equipment, and resin, keeping costs high and resulting in production bottlenecks in a very time-sensitive industry.


With the BioSMB multi-column chromatography platform from Sartorius you can get the best of both worlds. It offers significant savings without sacrificing any of the quality you expect, using essentially the same process in a more efficient way.


In downstream processing the resin represents a significant portion of the costs for single- column chromatography batch processes. However, at any given time with a single-column approach, only a small amount of the resin in the column is actually doing its job. The rest sits idle without being fully utilised and is eventually thrown away.


A better alternative is multi-column chromatography, which uses multiple small columns. These columns are cycled much more frequently utilising the resin fully. When you do more with less you can achieve up to 80% savings on chromatography resin costs with MCC. The MCC system is a direct replacement for a single column system.


Single-column chromatography facilities often limit throughput because using more resin to speed up the process costs more. Using less resin saves money, but it slows down the process and creates a bottleneck.


With multi-column chromatography each column is fully loaded, then once it’s complete that column can begin the purification process, which continues with each column cycling through various different stages in parallel. You get a more efficient process by performing multiple steps at the same time and increase productivity by 3-5x over single-column chromatography.


The switch from the standard large single column to multiple smaller columns is different, and the concept of column overload is new in most facilities. But neither will negatively impact quality attributes, so you get the same quality with more efficiency, all while reducing costs.


The MCC system enables the use of much smaller columns. You can configure as many or as few columns as needed to optimise your manufacturing process. But the size burden is always less, as is the storage and packing overhead. MCC systems eliminate the need for costly and large ancillary hardware traditionally required with conventional single-column approaches.


More information online: ilmt.co/PL/AwKZ 58203pr@reply-direct.com


INTERNATIONAL LABMATE - NOVEMBER 2022


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