Laboratory Products 59 Closed Loop Reaction Optimisation


Researchers at the University of Pretoria have developed a general platform for performing closed loop reaction optimisation by integrating a Uniqsis FlowSyn Maxi continuous flow reactor with an analytical HPLC.


In the reported work the FlowSyn Maxi was controlled over ethernet using an open-source Node-Red dashboard running on a Raspberry Pi linked to the single objective optimisation algorithm.


Dr Mark Ladlow, Uniqsis Chief Scientific Officer commented: “‘Optimising chemical reactions is an important but time-consuming iterative process. Flow chemistry affords an automated and precise method for performing chemical reactions that is well suited to performing autonomous reaction optimisation under computer control in a closed feedback loop using a suitable optimisation algorithm. Closed-loop control of a flow chemistry reactor is a sequential process whereby the result of each experiment is compared with the desired optimal outcome (in this case, the space-time yield of the reaction). A Bayesian optimisation algorithm then uses the new data to suggest an improved set of reaction conditions for the next experiment. Using real experimental data to update a probabilistic model for the reaction can allow the optimal outcome to be realised more quickly.”


The utility of the University of Pretoria open-source software approach, using the FlowSyn Maxi, was demonstrated by the semi-autonomous optimisation of a representative allylation reaction performed over 33 iterations in a 12-hour period. Beneficially, other Uniqsis flow chemistry instruments may be incorporated into the open-source dashboard to extend this approach to alternative system configurations, potentially capable of performing and optimising a wide range of chemical reactions.


Read the University of Pretoria paper in full: ilmt.co/PL/BlKZ Contact Uniqsis to learn more about closed loop reaction optimisation using a FlowSyn Maxi system. More information online: ilmt.co/PL/K2ea


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Expanded Range of Laminin isoforms


How to Protect Products from Aerosols, Dust Particles and Germs


All three groups of particles have one parameter in common and this is its hydrodynamic diameter, and if this is below 30 μm they can be transported over long distances or float in the room air for minutes. In production facilities humans are producers of aerosols (tiny droplets) and particles (skin particles and fibres from clothing), too. Many of these particles can impair product properties in production or packaging or even destroy optical or electronic components.


This is the reason why sensitive products must be protected from dust or micro- organism under cleanroom conditions and thus filter technologies are inexpensive and cost-effective in removing particles from air. This applies to entire clean rooms as well as to smaller filter units, so-called laminar flow boxes, because both systems are based on the same high-performance filters which can reduce particles (dust, microbes, germs, aerosols), because this process depends only on hydrodynamic but not on chemical or biological properties.


A laminar flow box, such as the FBS series from Spetec GmbH, has been tested by the Fraunhofer Institute for Manufacturing Engineering and Automation, and was classified according to DIN standard EN ISO 14644 in class ISO 5. This means that not more than 3,520 particles per 1 sqm can be detected inside the box improving air quality by at least 10,000 times.


Designed to efficiently induce differentiation in ES and iPS cells, AMSBIO has expanded its iMatrix series of recombinant laminin E8 fragments to now include isoforms LN-111 and LN-332.


This expanded range of laminin isoforms (LN-111, 332, 511, 411 and 221) now provides even greater flexibility and control when tailoring your substrate to the requirements of specific cultured cells.


Proven in many leading life science research labs - iMatrix is used as a substrate for culturing and maintaining cells, including Pluripotent Stem Cells and Mesenchymal Stem Cells, as well as for differentiation into ectoderm, endoderm, and mesoderm. All iMatrix products offer the dual advantage of recombinant protein expression and as E8 fragments they contain all of the integrin-binding sites of full-length laminin in a much shorter protein.


New to the product range, iMatrix-111 is the E8 fragment of the LN-111 isoform, which plays significant roles in vivo and is important in cell culture. As well as being the major substrate for nerve axon growth, both in vivo and in vitro, LN-111 is also the main component of the industry standard substrates used in cell culture: Matrigel®


and Cultrex® BME. iMatrix-111 has


been validated for to promote differentiation of iPSCs into hepatocyte-like cells.


The Laminin-332 isoform is essential for epithelial cell adhesion to the basement membrane and iMatrix Laminin-322 has been used to induce differentiation of iPS cells into corneal epithelial cells in vitro.


AMSBIO also offers a comprehensive range of synergistic high performance stem cell products for streamlined and efficient ES/iPS cell culture for basic to clinical research.


More information online: ilmt.co/PL/VEy0 and ilmt.co/PL/agMm


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All airborne microorganisms and dust particles are omnipresent in air. Thus, filter technologies are essential in food industry, optical, electronic and in pharmaceutical and medical production facilities, where also sterility plays an additional role, but again germs are omnipresent. Therefore, medical devices such as syringes, scalpels or bandages and plasters must be sterilely packaged and stored. Finally, we can conclude that air filters serve to protect our health and our products quality.


More information online: ilmt.co/PL/gPPm 58824pr@reply-direct.com


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