Spotlight Luminescence, UV & Image Analysis Fast Counting of Fluorescent Colonies and Plaques


Synbiosis is delighted to introduce ProcUV, its new UV imaging accessory for the ProtoCOL 2 system. ProcUV permits instant imaging of fluorescent colonies and plaques so they can be automatically counted or analysed by ProtoCOL 2, thus saving time and improving accuracy of results. Based on advanced fluorescent imaging technology, the compact ProcUV accessory, which can be simply connected to the ProtoCOL 2 system, consists of a cabinet with a sliding, auto-locking door to prevent accidental UV exposure. The cabinet contains a high-resolution camera and internal UV and white lighting and is also equipped with specialised interchangeable filters, to allow microbiologists to view fluorescing bacteria, such as Pseudomonas fluorescens, fluorescent plaques and bacteria expressing Green Fluorescent Proteins.


ProcUV is simple to set up as its automatic exposure time settings ensure users can capture colony images at the touch of a button. The high-quality images can then be directly transferred into the ProtoCOL 2 in seconds, where the ProtoCOL 2 counts and analyses results automatically, to save microbiologists countless hours of repetitive work.


Martin Smith of Synbiosis, stated: “Fluorescent colonies and plaques are the most difficult ones to count, as they require specific UV lighting conditions to be able to see them. This means that a powerful imaging system equipped with specialised filters to enable the camera to image each fluorescing colony is required.” Martin Smith, added: “We have risen to this challenge and by utilising the decades of imaging expertise we have in-house, we have come up with the perfect cost-effective solution in the ProcUV. For any microbiology laboratory wanting to extend the capability of their ProtoCOL 2 system to perform different types of fluorescent colony and plaque analyses, the ProcUV is ideal.”


Circle no. 257


The World’s First Multiple Wavelength Refractometer


Too Many Lines, not Enough Time


In ICP-AES, line-rich spectra are often observed and it is difficult to find for the analyte, a line free of interference among all the matrix or concomitant elements lines. It is particularly the case for matrices such as steel matrices, tungsten matrices, wastes with complex and various matrices. Usually the analyst prepares known solutions, makes profiles for each wavelength at different concentrations of the analyte and of the concomitant elements and select a line free of interference and with adequate sensitivity. This crucial step of the method development is long and tedious. It should be carried with great care because it influences the accuracy of the results.


Horiba Scientific simplifies this step with CLIP (Collection of Line Intensity Profiles), which assists the analyst in the development of methods for high resolution sequential ICP-AES: no more solutions to prepare, no more profiles to acquire. The profile of each line is calculated according to the instrument configuration: focal length, slits combination, diffraction grating and order used. Only few minutes are only needed to select lines for every element.


Each user of an ULTIMA family instrument can take benefit of CLIP, save time and get enhanced quality of the results. This module is available from March 2009, for every instrument of the ULTIMA 2 family currently ordered or already installed.


Analytik Ltd introduce the DSR-L from Schmidt + Haensch as the world’s first automatic refractometer to measure refractive index at multiple wavelengths. The use of discrete LEDs makes it possible to implement any combination of customer-specified wavelengths, allowing quasi-spectral measurements in almost real time.


The innovative DSR-L is extremely valuable in both R&D as well as quality control. It is ideal for measuring the refractive index dispersion of proteins, viscous materials, lenses, glass, thin film, and polymers and can be used to test product purity, characterise optical materials, identify counterfeits, and analyse quantities of dissolved solids like sugar, glycol and fats. By precisely measuring samples at seven separate wavelengths (400nm, 435.8nm, 486.1nm, 546.1nm, 589.3nm, 656.3nm, & 706.9nm), the DSR-L gives unique fingerprint-like data. There are many solutions that are chemically different but display comparable refractive index values at the traditional 589.3nm wavelength, however because the DSR-L can measure RI at multiple wavelengths, key differences can be obtained from otherwise similar samples.


The electronic Peltier device controls temperatures from +18 to +30°C and eliminates the need for an external water bath or thermostat. With accuracy of ±0.01°C, a temperature is easily set and the automatic refractometer quickly heats or cools to the target temperature. The DSR-L also features a long-lasting LED light source (100,000 hours), linear high- resolution CCD sensor, menu-driven backlit LCD displays, 4 user profiles, password protection, printer and data ports, and optional USB ports.


The included software package and RS-232C cable make it easy to connect the DSR-L to computers for collection of measurement data or for remote operation with full command of all functions. As new software versions are released, the instrument can also receive flash updates.


The DSR-L is suitable for ASTM D 1747 Standard Test Method for Refractive Index of Viscous Materials and ASTM D 1218 Standard Test Method for Refractive Index and Refractive Dispersion of Hydrocarbon Liquids.


Circle no. 260 Circle no. 258


Revolutionary Automated Full-Spectrum Fluorescence Microscope for Advanced Cell Analysis


Sartorius Stedim Biotech announced the introduction of ChemoMetec’s NucleoCounter® NC-3000™, a user-friendly system for fast


and precise automated detection of cells by specific counting of nuclei giving reproducible and accurate counts. The NucleoCounter NC- 3000 is an automated full-spectrum fluorescence microscope that offers a wide array of reliable and user-friendly assays with integrated collection, analysis and reporting of data. Validated for mammalian, yeast, insect and fish cells, the NucleoCounter NC-3000 is designed for applications including viability and cell count, cell cycle assay, mitochondrial potential assay, vitality assay, quantification of fluorescence intensity, fluorescence measurements from violet to far red, DNA fragmentation assay and GFP transfection efficiency assay. It will be of interest to those working in areas such as stem cell research, cell counting and viability, apoptosis and cancer, vaccine production, gene expression as well as transfection and cell cycle studies.


The system is easy to set up with no special training needed; users simply prepare the sample, load it into the NC-3000 and choose the


required assay. It is also extremely fast with an analysis time of 15-60 seconds (1µl sample) – this enables a complete viability assay to be performed within two minutes – including sample handling. Unlike flow cytometers there are no fluids within the NC-3000 which means no clogging, no spillage and no maintenance. Benefits of the NucleoCounter NC-3000 include: excellent precision and reproducibility; extreme ease of use; fast analysis (very short total operation time); automated data analysis and superior data visualisation software; calibration, maintenance and service free; all-in-one instrument with a small footprint; and plug & play system – preset parameters.


Circle no. 259 Window to the World of Gels


UVP, LLC is pleased to announce a unique upgrade to its PhotoDoc-It Imaging Systems for visualising gel images. Users can now observe fluorescent gels in the system via the unique viewport window. The window, mounted into the hood, blocks UV and allows researchers to safely see the gels when illuminated by the UV transilluminator.


The PhotoDoc-It Imaging System includes a high resolution 8.0 megapixel digital colour camera for capturing brilliant colour images. The camera attaches to the lightweight hood that sits on the transilluminator. No computer is required with this compact, stand-alone system. A small color printer connects directly to the camera supplying economical, lab quality 4” x 6” prints in seconds.


“The PhotoDoc-It Imaging System is configured for limited laboratory space and is designed as an economical replacement for Polaroid systems. The new viewer window allows researchers to see the fluorescently stained gels prior to capturing the images,” stated Alex Waluszko, Vice President for Marketing and Sales at UVP.


Circle no. 261


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