Figure 6 Conclusions
Liquid-Liquid Chromatography has matured into a valid science, which after almost 60 years deserves to be integrated into mainstream laboratory and process chromatography. L-LC been shown to compliment HPLC, with narrow range polarity cutting and by helping to find peaks co-eluting in HPLC. One beauty of L-LC is that the separation is based largely on defining on the polarities of targets, therefore classes of compounds can be separated which can then be optimised without sample loss. These narrow polarity range classes can finally be passed through a HPLC, assuming sample losses can be tolerated. If not, Sequential L-LC to L-LC with different solvents may be utilised. L-LC is a low-pressure technique (typically 100 to 500 psi) thus it can use lower price ancillary equipment than HPLC. L-LC usage has the potential for considerable solvent cost and timesavings.
References
[1] Leitao G.G, El-Adji S.S, Melo, W.A, Leitao, S.G, Brown, L., J. Liq. Chromatogr. Relat. Technol. (2005) 28, 2041-2051 [2] Leitao G.G, Souza, P.A, Moraes, A.A, Brown, L, J. Liq. Chromatogr. Relat. Technol. (2005) 28, 2053-2060 [3] Comprehensive Analytical Chemistry XXXVIII, A Berthod (Ed) (2002), Countercurrent Chromatography, Chapter 2, Berthod A, Brown L, Leitao G.G, Leitao. S.G [4] Silva J.C.T, Jham G.N, Oliveira, R.D. L, Brown, L., J of Chrom A (2007), 1151, 203-210 [5] Vidal S., Hayasaka, Y., Meudec, E., Cheyner, V., Skouroumounis, G.,
J.Agric. Food Chem. (2004), 52, 713-719 [6] Wangenaar F.I, Hochlowski, J.E, Pan, J.Y, Tu, N.P, Searle, P.A, J Chrom A, 1216 (2009) 4154 – 4160.
Figure 7
Figure 9
Figure 8
Figure 10
ADVERTORIAL Gas Generators for API LCMS
Both the ABN2ZA and the AB-3G Nitrogen Generators are specifically designed to operate LCMS Instruments manufactured by Applied Biosystems/ MDS Sciex. These new products produce Nitrogen & Dry Air through 3 independent outputs in the required flow rates, purities and pressures to cater for the requirements of all available Applied Biosystems/ MDS Sciex LCMS Models, including the new API5500.
Each of these new models essentially combine two separate gas generator systems in one unit with the source & exhaust gases (Dry Air) being generated by one system using one system and the curtain gas (Nitrogen) being generated by the other.
Internal air compressors make the ABN2ZA a stand- alone, self- sufficient Nitrogen Generator, independent from external air sources, whereas the AB- 3G has been designed with additional compressor capacity. This additional capacity allows us to cycle the compressors more frequently, maximising compressor life. In the unlikely event of one compressor failing, the internal control system will alert the user and manage the compressors to maintain a full output flow from the generator. This totally unique feature will allow the user to plan a service or repair visit with Peak Scientific, thus ensuring uninterrupted uptime of your LCMS.
the perfect match!
Network
Circle no. 19
Peak Scientific has tested these new models extensively at our manufacturing plant and with end users around the world to ensure reliability & longevity of the systems. For example, Borislav Starcevic, UCLA Olympic Analytical, USA commented about the AB- 3G:
“We had opportunity to test a new model of Peak Scientific Nitrogen generator for the last 6 months in our laboratory. Our laboratory is a busy lab as our instruments work 24/7. In the LCMS department of our laboratory we currently have 5 LC/MS/MS instruments all by AppliedBiosystems/Sciex: and we use nitrogen generators for all of them. We have four NM20ZA models and one N300DR model. The new model was connected to API4000QT which we use for service and for research. In comparison with the older models we found the new one to be less noisy and more reliable.
Overall we were satisfied with the unit and think that it is better than the older ones that we have in our laboratory.”
Applied Biosystems/ MDS Sciex have further approved these products to supply Nitrogen & Dry Air to all of their LCMS Applications.
Peak Gas Generators are unique in that they are simple to install & easy to use. After initial installation and set up, the generator will operate continuously with virtually no operator involvement & optimum performance can be maintained with an annual service visit.
Circle no. 20
Chromatography Focus
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76 |
Page 77 |
Page 78 |
Page 79 |
Page 80 |
Page 81 |
Page 82 |
Page 83 |
Page 84 |
Page 85 |
Page 86 |
Page 87 |
Page 88 |
Page 89 |
Page 90 |
Page 91 |
Page 92
