9 Online Calculator Converts Methods to New Core-Shell Column Technology
Phenomenex, Inc announced a new web-based tool that quickly optimises customer HPLC methods for use with the company’s new Kinetex core-shell columns. The specific benefits to be gained by switching from fully porous particle columns are also calculated. With the Kinetex Calculator, the researchers input their method running conditions and details regarding their current chromatographic results. In less than one minute, the calculator delivers an optimised method with the recommended Kinetex column and optimal run conditions for the user’s system, whether it’s HPLC or UHPLC. The calculator also provides expected backpressures, efficiency gains, solvent reduction, time savings and resolution improvements. The Kinetex calculator can process details of both isocratic and gradient methods. Delivering significant improvements in speed and separation efficiency over traditional 3- and 5-micron columns, Kinetex enables chromatographers to get performance comparable to sub-2-micron columns on existing LC instruments. With Kinetex, researchers can achieve UHPLC results on any LC system, completing chromatographic methods in a fraction of the time previously required, without sacrificing performance.
“With this new calculator, chromatographers are able to instantly preview the benefits Kinetex columns can bring to their specific methods,” said Jeremy Bierman, Brand Manager for Phenomenex. “This takes the guesswork out of choosing the best column for their method and adjusting their running conditions for optimum performance.”Initially, Kinetex core-shell columns are available in two particle sizes – 2.6- and 1.7- micron – and three chemistries – C18, PFP and HILIC. Kinetex 2.6- micron columns deliver separation efficiencies that match or exceed the performance of traditional fully porous sub-2-micron columns. The Kinetex 1.7-micron is the first ever sub-2-micron core-shell technology and delivers efficiency gains of 15 to 20% over traditional fully porous sub-2-micron columns. Method transfer challenges, common with traditional sub-2-micron products, are eliminated with Kinetex. Methods developed with either 2.6- or 1.7-micron Kinetex columns are scalable and can be transferred to any LC system using the 2.6-micron column, regardless of the instrument’s pressure capability.
Circle no. 21
HPLC System Upgrade Dionex Corporation is pleased to release the industry-leading Corona®
ultra™ Charged Aerosol Detector for the Dionex UltiMate®
3000 HPLC and RSLC systems. Charged Aerosol Detection®
(CAD®
) has
broad applicability across a wide array of applications. The Corona ultra can detect any non-volatile and many semi-volatile analytes independent of chemical structure, with or without a chromophore. This makes CAD useful for analytes ranging from small molecule pharmaceutical APIs and counterions to large molecules such as polymers and proteins. It is ideal for lipids using liquid chromatography where the wide variety of structures and widely varying UV response present an analytical challenge. The power of CAD lies in its versatility and ease of implementation. With integration into the UltiMate system, these capabilities are now available in an advanced HPLC system.
The redesigned Corona ultra is fully integrated into the UltiMate 3000 systems. Both detector and system are controlled by the Dionex Chromeleon® Chromatography Data System (patent pending) software.
With the analytical capability of the UltiMate 3000 RSLC systems, the ease of use of Chromeleon software, and the speed and low peak dispersion of the Corona ultra detector, this is an ideal combination for throughput, resolution and detection for all application where universal detection provides the answer to an analytical problem.
Circle no. 22
ADVERTORIAL The Pegasus® 4D GCxGC-TOFMS
The Pegasus 4D gives you four dimensions of analytical resolution for a significantly more complete analysis of complex samples. Separations, which previously resulted in dozens or hundreds of peaks, now yield thousands of individual components from complex mixtures. ChromaTOF®
4.20 software now features peak labelling for
both contour and surface GCxGC plots, graphing capabilities and Fisher Ratios that can be exported into peripheral software for additional analysis. Our new Statistical Compare feature and LECO/Fiehn Metabolomics Library work seamlessly with the Pegasus 4D to perform the classification of analytes and identify trends in sample groups.
Circle no. 23 ChromaTOF® 4.20 Now Available
ChromaTOF 4.20, Leco’s exclusive operating software for its separation science instruments, integrates acquisition, processing, and reporting tools into one total data solution.
• Enhanced graphics for GCxGC users, including the option of displaying peak labels and peak markers on contour and surface plots
• An improved user interface with tabs for easy access to common functions
• Statistical compare results and Fisher Ratios that can be easily exported and analysed by peripheral software for generating PCA plots
• Importable ready-to-use workspace templates for access to preferred user interfaces and reduced set-up times
ChromaTOF 4.20 also includes the same great functionality and time-saving features of previous software versions—including True Signal Deconvolution®
SCRIPTS, and EPA reporting tools. ChromaTOF 4.20 is standard with any new Pegasus®
HT GCMS, 4D GCxGC-MS, TruTOF® or GCxGC instrument. Upgrades for existing versions are also available. , Automated Peak Find, semi-quantitation, HT GCMS, Circle no. 24 The TruTOF® HT GC-TOFMS
The TruTOF offers continuous full-range mass acquisition rates up to 80 spectra/second for accurate identification of unknown and targeted compounds in complex samples. A unique benchtop design delivers all of the high-throughput benefits of GC-TOF in a space-saving package. Exclusive ChromaTOF® Deconvolution®
4.20 software with True Signal provides fast and easy data analysis and clean
library searches. The TruTOF also includes environmental reporting software, and offers both electron and chemical ionisation sources for increased laboratory productivity.
Circle no. 25 Circle no. 26
INTERNATIONAL LABMATE - FEBRUARY/MARCH 2010 - CHROMATOGRAPHY & SPECTROSCOPY
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