70 SKIN CARE
in which Vitis vinifera plant stem cells are subjected to a controlled fasting protocol. This condition prompts the cells to optimize their internal resources, recycle cellular components, and activate survival pathways, mirroring the biological mechanisms observed in calorie restriction models.6,7 To evaluate the impact of this fasting
process on the cellular secretome, a comparative metabolic profiling was conducted between secretomes derived from fasted and non-fasted cell cultures. The analysis focused on key metabolic markers, including peptides, proteins, sugars, polyphenols, and resveratrol, alongside their antioxidant capacity (Table 2). The results revealed that the fasting protocol
led to a marked increase in antioxidant activity, as well as a significant elevation in polyphenol content (including resveratrol) and peptide concentration. There are several studies that link resveratrol with its ability to modulate proteins directly related to longevity and stress response,8 restriction,9
enzymes involved in calorie and inflammation pathways.10,11 Vytrus’ innovative biotechnology subjects
grapevine stem cells to controlled nutrient deprivation, triggering a hormetic response to a positive mild stress that produces a bioactive secretome packed with:
Rich in polyphenols and secondary metabolites The fasting-induced secretome of Vitis vinifera stem cells is highly enriched in antioxidant compounds, particularly polyphenols, which are well known for their role in neutralizing oxidative stress and enhancing cellular longevity.17
These molecules contribute to the
overall protection and preservation of skin structure and function over time.
High content of natural plant peptides (signaling peptides and proteins) Grapevine secretome contains a diverse array
of signaling peptides and structural proteins naturally secreted by the plant cells. These bioactive peptides support skin regeneration, barrier reinforcement, and epidermal homeostasis by stimulating cellular repair and communication pathways.
A pool of natural plant exosomes The biotechnological process employed by Vytrus yields a secretome with a remarkably high density of natural nanometric plant exosomes—measured in the range of millions per millilitre of ingredient. These vesicles function as biological delivery systems, encapsulating and protecting key actives while facilitating targeted delivery and enhanced cellular uptake.
Exosomic resveratrol Unveiled by Vytrus Biotech, represents a breakthrough in understanding resveratrol’s role—not just as an antioxidant, but as a powerful signaling molecule. Naturally encapsulated in plant-derived exosomes during a fasting-mimicking process, it acts as a cellular switch, triggering longevity and regeneration pathways in the skin. This advanced delivery enhances its
PERSONAL CARE September 2025
bioavailability and mimics fasting effects, making it a key component in promoting skin health and resilience. Taken together, these findings suggest that nutrient deprivation triggers an adaptive metabolic reprogramming in the plant cells, enhancing the production of key bioactive compounds. Among them, resveratrol stands out not only for its antioxidant properties but also for its role as a signalling molecule capable of modulating critical cellular pathways related to health span and longevity, thereby reinforcing its relevance in promoting skin resilience and regeneration.
Biological activity (in vitro assays) A comprehensive series of in vitro studies was conducted to validate how grapevine secretome’s Plant Fasting Factors enhance resource optimization and modulate longevity- related biomarkers. Human dermal fibroblasts (HDFs) and
senescent melanocytes were subcultured for two months under different conditions: control, with a known hypoglycemic agent (C+; metformin), and grapevine secretome at different concentrations (0.65% and 2.5%). Key
Clarivine unlocks glass skin & longevity by: PIGMENTED SKIN GLASS SKIN
Clarivine's Plant Fasting Factors are rich in:
From plants to human, from fasting to longevity Figure 1: Bioactive profile of Vitis vinifera fasting-mimicking secretome
TABLE 2: FASTING-INDUCED CHANGES IN VITIS VINIFERA SECRETOME. COMPARISON OF KEY COMPOUND PRODUCTION AND METABOLIC MARKERS
Process of obtaining two types of secretome
+21.5% +80% +197% +136%
-45% -8%
findings underscore the biomimetic potential of grapevine secretome to induce metabolic optimization and regeneration in skin cells by means of the activation of the following longevity pathways: IGF-1 modulation: Treatment with grapevine
secretome led to a significant modulation in IGF-1 secretion in HDF cultures, indicating a metabolic shift that mirrors calorie restriction.12-14
While a modulation of up to
60% in IGF-1 levels was observed, this result suggests a potential downstream activation of FOXO pathways, which are known to regulate DNA repair, oxidative stress resistance, and longevity-associated gene expression.15,16 Autophagy activation: The conversion of
LC3-I to LC3-II, a widely accepted marker for autophagic flux, was significantly increased upon grapevine secretome exposure comparable to hypoglycemic effects of metformin (C+) (Figure 2). Telomere protection: Extended culture studies in HDFs revealed stabilization of telomere length in grapevine secretome treated cells, suggesting a protective role against replicative senescence. Protection against senescence: Enhances
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