54 HAIRCARE 40,000 *
100% stronger than the leading commercial benchmark
30,000 160 20,000 155 10,000 0 Untreated Placebo KeraBio K31
Animal Keratin
Benchmark
Figure 4: Graph showing the result of characteristic lifetime of three times bleached type 2 Caucasian hair treated with 20 applications from leave- on formulation (*p<0.05 compared to placebo formulation)
the cross-linking density and overall structural integrity of the hair’s internal keratin matrix. Damage to these matrix proteins leads to a decrease in TD , indicating a compromised hair structure.2
Methodology The impact of the biomimetic bond-builder on hair cortex integrity was assessed by measuring the denaturation temperature of three-times bleached, type 2 Caucasian hair. Hair was pre- cleansed with a standard shampoo and then treated for 15 minutes with either DI water (as a control), 0.5% active peptide solution, or 0.5% active animal keratin solution. The pH for both control and the active’s
solutions was adjusted to approximately 4 to ensure consistency. This application process was repeated ten times. Following treatment, hair was rinsed for 30 seconds and air-dried overnight. Baseline data was generated using 5-7mg of hair samples from all the different types and treatments cut in small segments of ~2 mm and suspended in water in a high pressure, high volume, hermetically sealed DSC pan. The DSC experiment for each sample was
performed between a range of 50 to 190°C, and with a heating rate of 10°C/min and the
165 160 155 150 Virgin Untreated Placebo KeraBio K31
Leading benchmark
Animal keratin
Figure 6: Graph showing the result of denaturation temperature of three times bleached type 2 Caucasian hair with 20 applications from a formulation (*p<0.05 compared to the control, + p<0.05 compared to the animal keratin
PERSONAL CARE September 2025 *
Figure 5: Graph showing the result of denaturation temperature of three times bleached type 2 Caucasian hair with ten applications from a solution (*p<0.05 compared to the control, + p<0.05 compared to the animal keratin)
data analysed to obtain the denaturation temperature. Testing was performed in triplicate and statistical significance of denaturation temperature for each treated hair sample was analysed using the ANOVA method.
Results Internal damage reduction Hair treated with this active exhibited a significantly higher denaturation temperature compared to the DI water control (p<0.05), indicating a substantial reduction in internal damage (Figure 5). Furthermore, the biomimetic peptide
notably outperformed the animal keratin treatment (p<0.05). This suggests that the ingredient reinforces the hair’s internal structure more effectively than animal keratin, leaving the hair with a stronger internal structure.
Reset” to Virgin Hair State: The study was also conducted from a formulation, this time after 20 treatments. The active’s formulation (5% active) was compared against virgin/untreated hair, bleached/untreated hair, a placebo formulation (no active), an animal keratin formulation (0.5% active), and a leading commercial bond builder benchmark.
The results (Figure 6) show that hair treated with this active exhibited a significantly higher denaturation temperature than all the benchmarks (p<0.05). Notably, the denaturation temperature exceeded that of virgin hair, suggesting that the ingredient reinforces the hair’s internal structure, effectively restoring it to a virgin-like state. In conclusion, the highly sensitive DSC data
consistently shows that this active outperforms all controls and benchmarks, both from solution and from formulation. This robust data validates its ability to strengthen the internal structure of hair, providing complementary and compelling support to the results obtained from the mechanical testing.
Conclusion Developed through an advanced biotechnology process, this human hair-identical keratin peptide is engineered for precise molecular- level interaction within the hair fibre. Rigorous scientific substantiation, including fluorescence microscopy, peptide binding assays, cyclic fatigue testing, and DSC, consistently demonstrates its ability to significantly enhance hair strength. This peptide outperforms all the benchmarks
tested, notably animal keratin and the leading commercial bond builder. These results substantiate the claim that KeraBio K31 delivers a true molecular-level keratin-recharge, elevating hair repair beyond conventional solutions and representing a significant advancement in the science of hair care.
References 1. Persistence Market Research. Hair Bond Multiplier Market. https://www.
persistencemarketresearch.com/market- research/
hair-bond-multiplier-market.asp
2. Wortmann FJ, Popescu C, Chandra J, Brunnschweiler J. Investigation of the swelling behavior of human hair in water and aqueous systems. J Cosmet Sci. 2002;53(4):219–28.
https://library.scconline.org/journal/journal-of- cosmetic-science/vol/53/issue/4/article/219
www.personalcaremagazine.com Leading 150 DI Water (Control) KeraBio K31 Animal Keratin * 165 *
PC
Denaturation temperature / °C
Characteristic lifetime (α)
Denaturation temperature / °C
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