6 Choose the Right Chromatography for your Application
Selecting the right chromatographic column and media is essential for a successful separation and purification. Chromatographers just like you use Kromasil slurry packed columns and bulk from the early stages of screening and discovery to impurity isolation and API production because they can employ the same Kromasil stationary phase material across their pharma and biotech organisations. With Kromasil’s unique value proposition, you can have the methods transferred seamlessly, to colleagues working in different scales saving weeks even months and come to market faster.
Kromasil, a brand of Nouryon, is produced with perfectly spherical silica-based materials with particle sizes from 1.8 to 25 µm. The diagram illustrates where best to use Kromasil products in your daily work and select from the Kromasil Classic, Kromasil Eternity, Kromasil Chiral and Kromasil SFC platforms.
More information online:
ilmt.co/PL/RDp1 50551pr@reply-direct.com
Safe and Reliable Gas Injectors for Moisture and Elemental Analysis
Fast and reliable measurement of various parameters in gases and LPG (liquified petroleum gases) is becoming increasingly important, as: water in LPG can cause valve and pipe freezing; already low ppm ranges of Fluorine and Chlorine in LPG can cause corrosion; Sulphur and Nitrogen in feedstocks and products need to be observed to maintain catalyst performance and for environmental reasons.
Nittoseiko Analytech, formerly Mitsubishi Chemical Analytech, provides gas injectors (GI) and vaporisers for gases (VG) that help to manage these challenges, guaranteeing safety and measuring reliability.
The VG-200, a standalone LPG sample injector for titration systems, doses a specified volume or mass of LPG into any titration cell or absorption solution automatically. The instrument is compliant to ASTM D7995 with the CA-310 Karl-Fischer-Moisture Meter.
For elemental analysis, the GI-220 is specialised to dose ambient or low pressure gases. The system capabilities include up to 1000 ml injection volume, and LOD down to 5 ppb Sulphur according to ASTM D6667 and D7551.
The GI-260 also simplifies elemental analysis and features fully automatic sampling and injection out of liquid phase LPG. Multi-Loop injection allows calibration out of one standard. The instrument is compliant to ASTM D7994 and UOP 1001, with combustion ion chromatography AQF-2100H.
More information online:
ilmt.co/PL/1JVO 53363pr@reply-direct.com
Effective Virus Particle Analysis Using Dynamic Light Scattering
Testa Analytical Solutions eK reports that Dynamic Light Scattering (DLS) can be used as an effective analytical technique for analysing virus particles in solution. A new application report examines four fish viruses incubated under laboratory culture conditions that have the potential to wreak significant economic damage to both recreational and commercially important fish populations.
Traditional visual methods of observing virus particles in solution give a snapshot of only a very small and potentially unrepresentative sampling volume. By comparison, the technique of particle analysis using dynamic light scattering yields an ensemble average of particles present in these solutions. Data presented in the report is from DLS measurements made on a NanoBrook ZetaPALS instrument equipped with a BI-MAS accessory to provide particle sizing capability.
The authors discuss how analysing virus particles incubated in the laboratory present a challenge, as they must be grown within cells in a media containing albumin and other small proteins, such as those present in Fetal Bovine Serum (FBS) solution. When virus particles are released from the cells, the cell fragments are large and can be separated by centrifugation, but the smaller proteins of the media cannot be removed. Careful selection of the distribution parameters from a dynamic light scattering experiment allows the size distributions of virus particles to be clearly observed in the presence of much smaller proteins that constitute the media.
The results presented in the applications report clearly show the presence of virus particles with the expected average dimensions when observed by an intensity weighted distribution. However the absence of these particles in the number distribution shows the virus particles are present in relatively small numbers compared to the proteins and other small molecules in cell growth media.
The Nanobrook ZetaPALS from Testa Analytical provides a platform for zeta potential determination of nanoparticles and colloids in water with salt concentrations less than 75 millimolar ionic strength. The instrument is designed to eliminate the shortcomings inherent in other zeta potential instruments.
For a copy of the application report please visit
ilmt.co/PL/Vkkm More information online:
ilmt.co/PL/a22W
53035pr@reply-direct.com
New Columns Separate a Wide Variety of Polar Analytes
Analysing polar compounds using liquid chromatography has historically been a challenge. Poor retention and peak shape, complex mobile phases that may not be MS-friendly, long equilibration times, low sensitivity, and sample derivatisation are all complications that reduce lab efficiency and productivity.
However, new Raptor Polar X columns reliably retain and efficiently separate a broad range of polar analytes using a unique stationary phase that balances two retention mechanisms: HILIC and ion exchange. This hybrid phase is ideal for analysing acidic, basic, and neutral polar compounds without time-consuming derivatisation or complex ion pairing, and it is especially compatible with mass spectrometry.
Easy changes to mobile phase conditions allow analysts to switch between modes and selectively tune retention for the compounds of interest without needing long equilibration times. Simplify the analysis of polar compounds with the resolving power of Raptor Polar X columns from Restek.
More information online:
ilmt.co/PL/2JKj 52693pr@reply-direct.com
INTERNATIONAL LABMATE - NOVEMBER 2020
Page 1 |
Page 2 |
Page 3 |
Page 4 |
Page 5 |
Page 6 |
Page 7 |
Page 8 |
Page 9 |
Page 10 |
Page 11 |
Page 12 |
Page 13 |
Page 14 |
Page 15 |
Page 16 |
Page 17 |
Page 18 |
Page 19 |
Page 20 |
Page 21 |
Page 22 |
Page 23 |
Page 24 |
Page 25 |
Page 26 |
Page 27 |
Page 28 |
Page 29 |
Page 30 |
Page 31 |
Page 32 |
Page 33 |
Page 34 |
Page 35 |
Page 36 |
Page 37 |
Page 38 |
Page 39 |
Page 40 |
Page 41 |
Page 42 |
Page 43 |
Page 44 |
Page 45 |
Page 46 |
Page 47 |
Page 48 |
Page 49 |
Page 50 |
Page 51 |
Page 52 |
Page 53 |
Page 54 |
Page 55 |
Page 56 |
Page 57 |
Page 58 |
Page 59 |
Page 60 |
Page 61 |
Page 62 |
Page 63 |
Page 64 |
Page 65 |
Page 66 |
Page 67 |
Page 68 |
Page 69 |
Page 70 |
Page 71 |
Page 72 |
Page 73 |
Page 74 |
Page 75 |
Page 76
