Chromatography 9 High Resolution Chromatography Flowmeter Announced
Testa Analytical Solutions eK has developed a new flowmeter which enables continuous measurement of flow rate without interference in chromatography systems.
Flow rate is one of the most important parameters in any liquid chromatography system, it determines retention time or volume and has by nature a major influence on reproducibility.
Compatible with all HPLC and GPC/SEC solvents, the new Testa flowmeter is conveniently sized and powers itself from a USB connection. A modern PC based app allows continuous recording and storage of the measured flow rates. The current flow rate is also displayed on the flowmeter’s integral high-resolution OLED Display, allowing easy control of current flow value.
Extraordinary high resolution and wide dynamic range makes the Testa flowmeter the perfect flow monitoring tool for the most demanding HPLC and GPC/SEC systems.
The new high resolution flowmeter is available as an off-the-shelf unit and also can be tailored as an optimised OEM module for chromatography instrument company’s interesting in taking advantage of this exciting new technology.
More information online:
ilmt.co/PL/1JAW 53392pr@reply-direct.com A Wettable HPLC Phase Explained
Recently the Kromasil wettable phase was introduced for production-scale purification of polar substances like biomolecules under aqueous conditions. It is now also available in columns for analytical HPLC applications. Nominally, the wettable phase is a C18 phase. Normally, this is a highly hydrophobic phase. How can such a phase be ‘wettable’?
On a regular C18 phase, if 100% aqueous conditions are gradually applied, the mobile phase can keep fairly good contact with the stationary phase as long as the high pressure in the system is maintained. In this situation the pressure forces the mobile phase to be present in the stationary phase’s pores, despite the repealing force from the hydrophobic surface. If the pressure drops (i.e. at flow decrease or stop), the liquid is expulsed from the pores, dramatically reducing the interaction surface between stationary and mobile phases. For the chromatographic properties that means a severe loss in selectivity and coelution of sample components. The surface availability can be restored in the column by switching back to normal phase conditions, though, but the actual chromatographic run can be considered as ruined.
To make the stationary phase wettable in fully aqueous conditions (avoiding dewetting as above), a polar component is introduced on the surface, like a mixed-mode phase. Some have added the polar groups at the root of the surface modifier. The Kromasil wettable phase has it as polar-embedded end-capping. This also allows for a better chemical stability of the phase under acidic conditions compared to other related purification phases on the market.
For more details about the chromatographic conditions for the illustration of this article, as well as learn more about this phase: properties according to the Tanaka test set, examples and current availability, please visit the wettable phase page on the Kromasil website.
More information online:
ilmt.co/PL/LK03 52864pr@reply-direct.com
Glass Autosampler Vials Virtually Eliminate Surface Activity
Reduced Surface Activity (RSA™) glass autosampler vials are not coated vials and virtually eliminate the adsorption of basic compounds found with all other glass vials. The manufacturing process of RSA™ vials produces vials without surface activity such as basic compound adsorption. Unlike ordinary glass vials, these vials will not produce a pH change with aqueous diluent over time in the vial and with minimised surface metals, it is excellent for LCMS in that it does not contribute to sodium adducts.
Laboratories testing low abundance analytes such as low dosage form pharmaceuticals, unknown unknowns and natural products will see the greatest benefit even though all labs will find these vials to have value when repeating runs or investigations must be avoided.
The vials are available from Microsolv in 12x32mm clear and amber with volumes of 2ml, 1.5ml and 300ul.
More information online:
ilmt.co/PL/ZJGm 52069pr@reply-direct.com
A harmonious performance across a wide portfolio
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