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2. G C Reilly, A J Engler. Intrinsic extracellular matrix properties regulate stem cell differentiation. J Biomech, 2010, 43(1):55-62.
3. F M Watt, P W Jordan, C H O’Neill. Cell shape controls terminal differentiation of human epidermal keratinocytes. PNAS, 1988, 85(15):5576-80.
4. C S Chen, M Mrksich, S Huang, G M Whitesides, D E Ingber. Geometric control of cell life and death. Science, 1997, 276(5317):1425-8.
5. R McBeath, D M Pirone, C M Nelson, K Bhadriraju, C S Chen. Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell, 2004, 6(4):483-95.
Figure 6a. Left picture: Mouse A9 fi broblast cells (labelled with Alexa Fluor 488 Phalloidin visualising F-actin in green) irregularly distributed on the surface of an EM grid. credit: Leica Microsystems
Figure 6b. Right picture: Fibroblasts adhering on fi brinogen micropatterns and therefore precisely positioned in the centre of the squares composing the mesh of a carbon EM grid. credit: Alvéole, collaboration with Dr K. Grünewald
preparation. However, microcontact printing and photopatterning with masks are very hard if not impossible to perform on EM grids due to their size and delicacy.
Several research teams have recently successfully micropatterned cells on EM grids using the contactless PRIMO micropatterning system and published their results showing unrivalled performances compared to other micropatterning techniques [13]. They were able to prepare better cell samples for cryo-ET experimentation, with cells well isolated and at the desired locations [14] and also emphasised that the impact of micropatterning on the organisation of the cytoskeleton could help predict the intra-cellular locations of some cytoskeleton components and improve the throughput of data acquisition, allowing for more effi cient cryo-ET studies [15].
Integrating this new micropatterning method to the ongoing improvements in cell sample preparations for cryo-ET, including plunge freezing, correlative light microscopy, cryo-FIB lamella milling, as initiated with a partnership between Alvéole and Leica Microsystems, will soon accelerate research breakthroughs on what really goes on in the cellular machinery at the molecular scale.
6. M Théry. Micropatterning as a tool to decipher cell morphogenesis and functions. J. Cell Sci., 2010, 123(Pt24):4201-13.
7. A Rigort et al. Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography. PNAS, 2012, 109(12):4449-54.
8. P-O Strale, A Azioune, G Bugnicourt, Y Lecomte, M Chahid, V Studer. Multiprotein Printing by Light-Induced Molecular Adsorption. Adv. Mat., 2016, 28(10):2024-9.
9. V Ruprecht et al. How cells respond to environmental cues - insights from bio- functionalized substrates. J Cell Sci, 2017, 130(1):51-61.
10. S R Polio et al. Extracellular matrix stiffness regulates human airway smooth muscle contraction by altering the cell-cell coupling. 2019, 9(1):9564.
11. X Luo et al. Lymphocytes perform reverse adhesive haptotaxis mediated by LFA-1 integrins. J Cell Sci, 2020, 133(16):jcs242883.
12. Alvéole users’ scientifi c papers:
https://www.alveolelab.com/resources-support-center/ scientifi c-papers/
13. L Engel et al. Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies. J Micromech Microeng, 2019, 29(11):115018.
14. M Toro-Nahuelpan, L Zagoriy, F Senger, L Blanchoin, M Théry, J Mahamid. Tailoring cryo-electron microscopy grids by photo-micropatterning for in-cell structural studies. Nat Methods, 2020, 17(1):50-54.
15. L Engel et al. Lattice micropatterning of electron microscopy grids for improved cellular cryo-electron tomography throughput. BioRxiv, 2020
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New UV Ring Light for Stereo Microscopy Launched The new SCHOTT®
VisiLED UV Ring Light for stereo microscopes combines classic bright-fi eld illumination with UV illumination. It is the only segment
ring light on the market in which white-light and UV-LEDs are alternately installed in eight segments. This LED arrangement allows objects to be examined from the same illumination angle, which signifi cantly improves the ability to compare and reproduce resulting images. While the homogenous white light displays samples in natural colour, UV excitation reveals previously invisible parts of the sample through fl uorescence. The SCHOTT MC 1100 Controller (available for separate purchase) makes it easy to switch between the two illumination modes.
The UV Ring Light for stereo microscopes is used in applications such as forensic document examination, crack detection or examination of paints and varnishes. With fl uorescent markers and UV light, previously invisible details can be made visible. A UV protection fi lter in the observation path ensures that the user can look safely at the sample through the eyepieces and clean operation of the microscope camera.
“We were increasingly asked by customers for UV illumination that is safe and easy to use,” said Dr Christian Zawar, Senior Sales Manager at SCHOTT Lighting and Imaging. “In addition, the user always needs a normal bright fi eld illumination on the microscope. Our new VisiLED UV ring light combines both of these capabilities in a unique way.”
The VisiLED UV Ring Light contains UV-A LEDs that emit wavelengths between 340 and 420 nm, with a central wavelength of 375 nm. The new UV ring light offers an illuminance of 100 klx in bright-fi eld mode and 160 W/m² in UV-illumination mode. Each ring light comes equipped with the SCHOTT GG435 UV Protection Filter. Although the segment ring light belongs to risk class 3 according to IEC62471, the fi lter ensures maximum safety for the eyes when looking into the eyepieces.
More information online:
ilmt.co/PL/6Pa3 52613pr@reply-direct.com
New Remote Live-cell Imaging System Launched
CytoSMART Technologies has announced the launch of a new automated live-cell imaging system designed for long-term experiments, comparison studies, and large laboratory teams. The CytoSMART Multi Lux is a cost-effective solution for researchers who want to carry out side-by-side comparisons between cell cultures, run long-term experiments, and monitor cells from their home’s comfort.
Joffry Maltha, CEO at CytoSMART Technologies, said: “An advanced live-cell imaging system consisting of four mini digital microscopes supported by automated image analysis software is especially useful for research groups that have been negatively impacted by COVID-19 restrictions and want to fi nd new ways to continue their research. No matter how big your laboratory team is, this new Multi Lux technology lets scientists run up to four experiments simultaneously from inside the incubator at perfect culture conditions. Automated image analysis and immediate visualisation of the results are accessible remotely via the CytoSMART Cloud. Besides, researchers who need to culture cells for weeks or even months, can also benefi t from this long-term live-cell imaging solution without fear of needing to throw away cells because of failed experiments.”
The main features and benefi ts of the CytoSMART Multi Lux include: four sample stages for simultaneous side-by-side comparison; long-term non- invasive, label-free image analysis; full remote access: possibility to inspect cell cultures while working from the comfort of home; suitable for large laboratory teams; cost-effective solution: four imaging devices connected to a single laptop, including unlimited storage.
More information online:
ilmt.co/PL/ROnR 53498pr@reply-direct.com
INTERNATIONAL LABMATE - NOVEMBER 2020
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