59
Looking at the images in Figure 2 (a and b), one can see the sweat ducts in the vertical detail component image. There are indications of sweet duct and sweet gland in the horizontal detail component images of dB1 produced by either DWT or SWT. Stratum corneum can be detected easily from the horizontal detail component image produced by either dB1 or Sym4. The images produced by wavelet mother function dB1 seem to be more informative than those produced by Sym4. The vertical and horizontal components obtained from the DWT compared to those from SWT do not carry significant information. (see Figure 3 (a and b). In both images produced by stationary and discreet wavelet functions, the diagonal detail component image does not include much useful information. Using SWT, with dB1, the gradient images of the horizontal and vertical components are more informative than their approximation component. With SWT, sym4 does not demonstrate many details in the images. With DWT, this is the case with dB1. The horizontal and vertical components produced by sym4 are more informative than those by dB1. We only demonstrated the results of the transformation in the second decomposition level as with this level of decomposition the best results were obtained. The information that was obtained in the gradient image is helpful for the investigation of skin structure.
The preliminary results presented here, showed that the wavelet components and their gradients can add more details to the OCT skin images. This eventually helps the dermatologist to make a better decision in diagnosis.
References:
[1] A. Manjunath and H. Ravikumar, "Comparison of Discrete Wavelet Transform (DWT), Lifting Wavelet Transform (LWT) Stationary Wavelet Transform (SWT) and S- Transform inPower Quality Analysis," European Journal of Scientific Research, vol. 39, pp. 569-576, 2010.
[2] M. Hughes and A. G. Podoleanu, "Simplified dynamic focus method for time domain OCT," Electron. Lett., vol. 45, pp. 623, 2009.
[3] R. M. Rao, A. S. Bopardikar and T. Boros, "Wavelet transforms: Introduction to theory and applications," Journal of Electronic Imaging, vol. 8, pp. 478, 1999.
[4] J. Schmitt, "Optical coherence tomography (OCT): a review," Selected Topics in Quantum Electronics, IEEE Journal of, vol. 5, pp. 1205-1215, 1999.
First Published in Lab Asia July/August 2011
New Gel Documentation System For Quick and Easy Imaging
Syngene recently introduced its new GENi2 gel documentation system, the next generation version of its affordable motorised GENi range. This point and press system is ideal for scientists wanting to produce accurate images of fluorescent DNA gels, as well as fluorescent protein gels in seconds.
The versatile GENi2 can be used for many DNA and protein gel imaging applications. The system features a compact darkroom containing a 2 mega pixel resolution camera, motor-driven lens and six position motorised filter wheel, all controlled by an internal high speed processor.
To quickly set up image capture, image manipulation and even image rotation, researchers use an integrated intuitive touch-screen rather than an external computer. This means the system takes up minimal bench space, yet still has all the features necessary for rapidly generating high quality gel images.
The cost-effective GENi2 darkroom comes complete with a slide-out trans-illuminator and white light table for visualising DNA and protein gels. An internal white light makes sample positioning simple and the interlocking doors automatically turn off the transilluminator when open, keeping users safe by preventing UV exposure.
To archive or analyse their results, researchers can save their images to a memory stick or network connection. Then by using GeneTools, Syngene’s highly regarded image analysis software, which also comes free of charge with a GENi2, scientists can accurately analyse their images.
Laura Sullivan, Syngene’s Divisional Manager, said: “Many researchers need a versatile, click and capture gel doc that can image a much wider range of DNA and protein gels than they did five years ago.
“Our new GENi2 is that system, and we will be delighted to demonstrate why GENi2 is an exceptionally good value gel imager to any scientists that contact us today.”
Circle no. 216
Interested in publishing a
Technical Article?
Please contact: Gwyneth Astles on+44 (0)1727 855574 or email:
gwyneth@intlabmate.com Circle no. 217
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