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CHROMATOGRAPHY
New Technology Assists Development of State-of-the-Art Pediatric Healthcare
Porvair Filtration Group has announced that the Childrens Research Institute at the Medical College of Wisconsin has used the company's Chromatrap™ technology to develop more efficient and more precise Chromatin Immunoprecipitation (ChIP) assays. The Children's Research Institute is renowned for its state-of- the-art pediatric studies. The CRI's translational research programs are targeted to find life-saving discoveries and cures in the diseases that affect children and interventions that enhance quality of life for children and families living with chronic health conditions. Over the last few months - Dr Ronald N Hines, Professor of Pediatrics, Pharmacology & Toxicology at the CRI has compared the efficacy of Chromatrap™ with traditional bead based ChIP technologies in his research into known regulatory elements controlling ontogeny of important human drug metabolising enzymes, such as CYP3A4. He commented: "I believe the bead-based system gave us a relatively small signal to noise ratio such that even minor deviations caused by variation in the efficiency of the bead system (for whatever reason) resulted in considerable variation in the assay". He added: "By comparison I think the most substantial advantage of the Chromatrap system is more efficient and less variable trapping of the immunoprecipitated chromatin such that the signal to noise ratio is larger and influenced less by minor variations in trapping efficiency."
The Chromatrap™ spin column approach offers significant advantages compared to methods based on agarose or magnetic beads, which involve many steps of separation, pipetting and re-suspension. The BioVyon™ protein A discs used in Chromatrap™ spin columns have a proprietary internal structure, composed of pores that have inner surfaces specifically designed to maximise chromatin capture efficiency. Porvair's proprietary disc base material is chemically inert, reducing incidences of non-specific binding. All chromatin capture reactions take place inside the rigid porous disc; therefore there is no need for the many repetitive stages of suspension, collection and resuspension by pipetting. Additionally no pre-blocking step is required. The Chromatrap spin column protocol allows washing steps that are fast, efficient and less prone to error and the 'flow through' characteristics of the column ensure excellent sample mixing and washing during the assay.
Circle no. 51
Highest Performing UPLC Ever Engineered Waters Corporation introduced the new Waters®
AcquityUPLC®
I-Class System, the highest performing UPLC®
system ever engineered taking UltraPerformance LC®
separations technology to the next level. For
scientists challenged by the most difficult separations, the AcquityUPLC I-Class features the lowest system dispersion and lowest carryover in the industry today. Low dispersion maximises peak capacity thereby advancing chromatographic separations and optimising the performance of any mass spectrometer. Keeping pace with today's most advanced laboratories, the AcquityUPLC I-Class System excels when analysing complex, sample-limited compounds. Sample-limited compounds include high potency medications, trace levels of food or water contamination, and (advanced) sophisticated biotherapeutics. These samples can represent complex separation challenges requiring an LC system that can maximise the benefits of sub-two-micron for faster throughput and optimised MS performance.
While there are many innovations built into the new AcquityUPLC I-Class, minimal system volume is a new key feature. Reduced system volume significantly decreases dispersion rates for reproducibly higher resolution and superior peak capacity. Reduced dispersion and small peak volumes extend the sensitivity of any mass spectrometer. Additionally, low dispersion footprint allows users to reduce their separation cycle times without impact to the separation as resolution is maintained even for ballistic gradients lasting less than one minute. Also importantly, new design and materials dramatically reduce sample carryover effects, thus increasing user confidence in results. The AcquityUPLC I-Class also features class leading flexibility and precise sampling capabilities. Depending on application needs, users can choose either the highest precision and lowest dispersion fixed-loop Sample Manager, or the world's first low dispersion variable volume Sample Manager with a flow-through needle design, which delivers high-precision injections, excellent sample recovery, and lowest sample carryover optimising the performance for even the most sensitive mass detectors.
Circle no. 52 ADVERTORIAL Whisper Nitrogen Generator
The Whisper Nitrogen Generator has been developed using the membrane technology to meet the specific requirements in terms of flow, purity and pressure in LC-MS applications. It can also be used for the evaporation of solvents in samples being analysed.
The model exists in different flow rates, with or without internal compressor. A specific Model called Whisper Hybrid has been specially designed for ABI LC-MS applications.
Circle no. 54 Hydrogen Generators
LNI Schmidlin SA has developed different type of Hydrogen Generators: The Classic line NM-H2-series is ATEX certified and has capabilities for cascading. The New Tech line H2-FID and H2-Carrier has a Touch pad display and an innovative water management system.
Both models employ’s the newest membrane technology available and includes the exclusive no maintenance auto drying technology. The pure gas (99.9999%) is ideal for operation with gas analysers, as fuel gas for flame tools, all kind of carrier Gas application, like GC and GC-MS (Mass Spectrometry), and as safe source of pure hydrogen in plasma chambers and other isolated environments.
Circle no. 55 LAB Air Stations
LAB Air Stations are available in 3 versions: AIR STATION: includes a quite built-in air compressor for to supply oil free and dry air.
ZERO AIR /ZERO AIR STATION: includes (for the STATION) a built-in compressor for to supply oil free and dry air. A catalytic oven removes the hydrocarbons down to 0.1 ppm
ULTRA ZERO AIR / ULTRA ZERO AIR STATION: includes (for the STATION) a built-in compressor for to supply oil free and dry air. A catalytic oven removes the hydrocarbons down to 0.1 ppm and in
addition a set of specific PSA filters to remove the CO2, CO, HC, NOX, SO2 and O3 contaminants and moisture down to 0.1 ppm.
Typical flow rates for the instruments are from 3L/min to 30 L/min. The uses of the LAB AIR STATIONS reduce the operating costs significantly.
Circle no. 56 H2-AIR FID Station
The LNI Schmidlin SA hydrogen generator is the economical answer to high-pressure gas cylinders in the laboratories.
Hydrogen and Air are generated from the same unit, ready to feed any kind of Flame Ionisation Detectors (FID). Zero Air is made from compressed Air from the laboratory and Hydrogen is made by electrolysis from the deionised water. The FID station is very small in food print and easy to operate.
Circle no. 53 Circle no. 57
INTERNATIONAL LABMATE - AUGUST/SEPTEMBER 2011 - CHROMATOGRAPHY
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