47 CCD System up to 10 Times Faster than Laser Scanner for IR Image Analysis
Syngene announced the results of a head-to-head study carried out at the University of Cambridge to demonstrate that the G:BOX Chemi IR6 can produce images of IR labelled Western blots up to 10 times faster than laser based scanning. The G:BOX Chemi IR6, which is Syngene’s new multi-application CCD imaging system for analysing IR and fluorescently stained proteins, was tested against a laser based scanner. Both imagers were tested side-by-side in a comparison study at the University of Cambridge for analysing proteins on Western blots labelled with DyLight®
680 and DyLight® 800 dyes (Thermo Fisher Scientific).
The results, detailed in Syngene’s Application Note 62, show that using the G:BOX Chemi IR6 with Epi Red lighting and a 705nm filter, an image of DyLight 680 labelled bands can be captured in just 20 seconds. With Epi LED IR740 lighting and a LY800 filter, an image of the DyLight 800 labelled proteins can be generated by the G:BOX Chemi IR6 in two minutes. In comparison, the laser based scanner can take more than 10 times longer to image DyLight 680 and twice as long to image DyLight 800. The systems had comparable sensitivity, with both their limits of detection being 0.11µg.
Lindsey Bunn, Syngene’s Application Specialist, explained: “With laser-based scanners imaging times are determined by scanning resolution and intensity setting, whereas using a G:BOX Chemi IR6, imaging times only depend on sensitivity. This is why we believed our CCD system would be faster than and just as accurate as a laser based system and are delighted that the results of the study have proved this.” Lindsey continued: “The added benefit with the G:BOX Chemi IR6 is the system can analyse all commercial fluorescent, chemiluminescent and visible dyes as well, making it a more cost-effective purchase. Researchers who need to rapidly produce images of multiplex labelled Westerns but whose labs don’t have the budget for an expensive laser scanner should consider testing a G:BOX Chemi IR6 system today.”
Circle no. 166 Vacuum Control Package For Evaporation of High-Boiling Solvents
For fine vacuum distillation with high-boiling solvents in most cases the required vacuum condition is specified, while the temperature is kept as variable process parameter. Fluctuating parameters can lead to difficulties in keeping the vacuum constant and therefore complicate the achievement of reproducible results. The new Vaccuubrand control packages for rotary vane vacuum pumps allow significantly optimised or even automated evaporation processes and increased evaporation rates. The vacuum is kept nearly constant at the vacuum set point using a solenoid-operated vacuum valve. Therefore even solvent mixtures can be separated sufficiently. The user achieves improved results at minimised process time. Vaccuubrand offers two new vacuum control packages for small flange connection KF DN 16 or KF DN 25. The vacuum control packages are usable for single- and double-stage rotary vane pumps with pumping speed capacities according to the user’s requirements. The packages include all required components (except the pump itself). The vacuum controller CVC 3000 with chemically resistant fine vacuum sensor VSP 3000, a solenoid-operated vacuum valve, as well as required components for connection, can be adapted to existing or new rotary vane pumps. The controller regulates process vacuum on demand in the range from atmospheric pressure down to 10-3 mbar. The ceramics-embedded measuring element of the vacuum sensor VSP 3000 (Pirani-type) features outstanding chemical resistance as well as mechanical stability. Accidental sudden venting, which can damage the highly sensitive internal measuring filament of a traditional Pirani-sensor, doesn’t harm the new VSP 3000. Included in the control packages are the vacuum controller CVC 3000 with controller support for bench top use or mounting at support rods, the fine vacuum sensor VSP 3000, as well as a chemically resistant solenoid- operated vacuum valve VV-B 15C and a T-connector with corresponding small flange connections, required centering and clamping rings as well as a hose nozzle adapter.
Circle no. 167 ADVERTORIAL
General Laboratory Application Refrigerated/ Heated Circulators
The newest Thermo Scientific thermostat line is designed as the perfect fit for many applications in the lab from -50 to +300°C. The HAAKE Arctic refrigerated baths and HAAKE Sahara heated baths can be combined with 3 performance types of control heads, the HAAKE Standard, Advanced and Premium heads, building versatile temperature control systems for various open bath applications (beakers, racks, probes), or precise cooling and heating of photometers, rheometers, electrophoresis or other lab equipment.
A key focus of the thermostat design was the intuitive user controls and an energy saving feature for all refrigeration systems. The control head can be oriented to all 4 sides on the bath giving great flexibility for the setup in the lab.
All systems are UL/CSA & CE compliant and manufactured in superior quality which is underlined by 3 years warranty and supported worldwide by Thermo Scientific.
For more information, visit
www.thermo.com/bathad The perfect fit. The power to change your world is clear. Circle no. 168
Thermo Scientific NESLAB Recirculating Chillers Provide Years of Worry-Free Operation
The Thermo Scientific NESLAB ThermoFlex recirculating chiller platform was developed with customer input from concept to design. The result is an easy-to-use, easy-to-maintain high performance chiller platform configurable to the most demanding applications. The ThermoFlex chillers maintain process temperatures from +5o
C to +40o C with stability of +/-0.1o
and cooling capacities up to 20000 watts. Designed as a modular platform, the ThermoFlex line offers a wide variety of options and cooling capacities that can be configured for diverse applications in markets such as analytical, biotech, optical, medical, microscopy, laboratory, laser, metrology, and pharmaceutical. Each chiller includes air and water filters that can be changed while the unit is in operation and an intuitive user interface for ease of operation. End-users benefit from innovative, patented packaging for rapid installation and a quick start guide for seamless start-up in minutes. User-adjustable alarms protect your application. An extended temperature range of +5o
C to +90o C is available as well as a deluxe controller option which offers a Multi-line LCD Display.
Thermo Scientific also provides the Merlin Low-Range Recirculating Chiller series for process temperature from -15o stability of +/-0.1o
C to +35o C. For more information, visit
www.thermo.com/thermoflex Circle no. 169 Circle no. 170 Moving science forward C with C
Thermo Scientific temperature control systems combine a new generation of thermostat technology with the most extensive line of proven baths in the industry for your perfect fit.
Match the best thermostat and bath for more than 400 cus- tomized, feature-rich systems to meet your precise application and budget requirements. Available in heated, refrigerated and cryogenic product families, our control systems deliver new levels of performance and flexibility, plus:
• Lower operating expense: advanced thermostat designs reduce energy requirements for significant savings
• Safe operation: each unit is CSA/UL compliant, assuring safe operation under any fault condition
• Total peace of mind: each system is backed by our global support and service for worry-free operation
Tune your control solutions as precisely as your temperatures. Download our new brochure at
www.thermo.com/bathad
Thermo Scientific temperature control systems:
Next-generation temperature control for your heated, refrigerated and cryogenic applications
INTERNATIONAL LABMATE - AUGUST/SEPTEMBER 2011 - LABORATORY PRODUCTS
© 2010 Thermo Fisher Scientific Inc. All rights reserved.
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