Passing the test
nity to see how easy the tests were to run, how stable the test kits are, and to direct any questions to our team to experts. ‘It was an excellent forum. The feedback we received indicated the
event fulfi lled expectations and was well received by industry and reg- ulatory professionals’, she continued. ‘Offi cials from the FSAS were also pleased to see the collaboration between industry and technology in tackling the challenges we all face in our sector.’ The testing technology that was highlighted at the workshop included: Reveal 2.0 for DSP, a single-step immunochromatographic lateral fl ow
assay, used for the rapid and practical qualitative analysis of shellfi sh possibly contaminated by okadaic acid (OA) group toxins (OA, DTX1, DTX2 and DTX3). OA is produced by marine dinofl agellates, such as Dinophysis, and has structural analogues referred to as the dinophysis- toxins (DTXs). The test is compatible with both European Commission (EU) and US Food and Drug Administration (FDA) permitted levels. The test can detect as little as 160ppb of OA toxins in shellfi sh sam-
Left: Some of the delegates at the marine biotoxin workshop. Above: Demonstrating the testing kits
The main focus of the workshop was a demonstration by Neogen of the latest technology and methods to test for shellfi sh toxins, including the Reveal 2.0 for PSP and, in particular, the DSP hydrolysis extraction. Delegates were keen to see the process in action and to understand how it could be adopted into their test plans. The Neogen team was able to effectively demonstrate that by simply utilising a heat step, and with the addition of two solutions, hydrolysis would allow certain toxins to be detected by the strip, which otherwise wouldn’t be seen. There was acknowledgement by industry personnel that hydrolysis extraction was simple enough to be carried out on location, and many present were keen to adopt it into their own test plans. Commenting on the workshop, Dr Janice McGregor, Neogen Europe’s UK & Ireland Divisional Sales Manager, said: ‘The workshop gave delegates an opportu-
ples, within 15 minutes from extraction. After weighing and homogenis- ing your sample, you simply remove 100 uL of your sample, add buffer, transfer to a microwell and place a strip into the well. After 15 minutes you insert the strip to the reader, which will then provide your results. Reveal 2.0 for PSP, which detects PSP-causing toxins at a level of 800
parts per billion (ppb),is compatible with EU and FDA permitted levels. This new shellfi sh toxin test can be used in the fi eld or in a lab and is designed for use by harvesters and processors to test mussels, scallops, oysters, clams and cockles. Toxins that cause PSP can be produced by dinofl aellates of different genera, including Alexandrium and Gymnodini- um; the most researched of these toxins is saxitoxin (STX). To complement the range of on-site testing, Neogen is also able to
provide contract laboratory services for both shellfi sh toxins and noro- virus, using methods such as RT-PCR, HPLC-PDA, LCMS-MS and ELISA. For further information on Neogen’s range of shellfi sh toxin testing and contract laboratory services please call 01292 525625 or email shellfi
sh_uk@neogeneurope.com FF
Quality Testing for Quality Seafood Neogen provides rapid and simple to use tests for the detection of
Amnesic (ASP), Diarrhetic (DSP) and Paralytic (PSP) toxins in shellfish. • Results within as little as 5 minutes • Tests can easily be used in the field or lab • Powerful and permanent reporting,
tracking and trend analysis with AccuScan® • Full technical support provided
Tel: 01292 525 625 •
www.neogeneurope.com •
shellfish_uk@neogeneurope.com www.fishfarmer-magazine.com 55
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