6 Improving Aqueous Sample Concentration in Pharmaceutical Labs
The Department of Biomedical Pharmaceutics, headed by Professor Kiyoyuki Kitaichi, is well known for its pioneering collaborative research on illegal drugs, pharmaceuticals, and new health food ingredients. However, traditionally Professor Kitaichi’s laboratory staff had relied upon using nitrogen blowdown equipment to concentrate aqueous samples. Drying low volume samples was especially problematic as the method required adding organic solvent several times to enable water to be fully removed - a methodology that took up to 60 minutes and consumed a lot of nitrogen gas.
Professor Kitaichi commented: “We typically start our sample preparation by adding organic solvent and water to biological samples such as blood, tissue, drug products, natural product extracts, and then run an extraction process. The resultant aqueous sample extracts, containing drugs, their metabolites or health food ingredients are then concentrated or completely dried prior to the sample being redissolved in mobile phase and injected into HPLC or LC-MS for analysis. Using our Smart Evaporator C1 from Biochromato it is now possible to concentrate aqueous samples quickly and easily, which was a problem with the nitrogen blowdown equipment. Our concentration process now takes just minutes rather than an hour and eliminates the need for wasteful use of nitrogen gas. The new Smart Evaporator concentration methodology is especially useful for compounds which have a high affinity for water. To further increase our productivity we also invested in a kit (4-Kit) for use with the Smart Evaporator C1 that allows you to concentrate 4 samples at a time.”
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Direct Adaptive HPLC Column Connectors
When changing from a Waters column to another brand like an Agilent Column, do you find changing the ferrules and tubing (to maintain data integrity) time consuming? If so, Microsolv Technology Corporation have a solution that can help, introducing the MicroSolv™ Direct Adaptive (Di-Ad™) HPLC Column Connecters. They make this process much faster and easier without fussing with ferrules and tubing between column changes.
These Direct Adaptive Fittings were designed for you to avoid poor column connections and eliminate extra system volume. The universal pilot length of this spring-loaded connector adapts to any HPLC column for quick column swaps and prevention of poor connections that cause peak broadening. Available as a Single End Fitting or a Double End Fitting. You just simply screw the Di-Ad™ fittings into the column and the tubing pilot length will automatically be adjusted.
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New Module Enables Automated Method Modelling As a significant expansion of DryLab®
module - the DryLab®
4’s functionalities the team at Molnár-Institute announces the release of the software’s newest Empower Automation.
This module enables automated method modelling on three levels of DryLab’s AQbD-workflow: it creates, performs and retrieves input runs, automates the model’s verification and validates its in-silico robustness assessment.
The automated and seamless integration between DryLab® 4 and Waters Empower equips the chromatographer with an easy-to-use
modelling platform. It allows for science-based, flexible decisions in modelling, mitigated risk, improved analytical throughput and a holistic approach to state-of-the-art method development.
The Empower module generates method sets and runs them in the most economic and ecological order, taking into account subsequent equilibration steps. DryLab® then accesses Empower’s data-stream for additional peak-information on peak shapes (Tf, w0.5) and peak names that can be directly used to adjust the model.
Compliant with the recently introduced ICH Q12 guideline for assessing the robustness of an analytical method the new DryLab® between drug discovery and approval by optimising use of instrumentation and determining the most robust method conditions.
Empower Automation further facilitates the R&D process to reduce time
The Empower Automation Module further supports peak tracking using MS data where Waters QDa or SQD2 are used. In simple cases a completely automated peak tracking is possible. In other cases, manual peak tracking is supported by displaying mass spectra for selected peaks or plotting single ion traces over the UV chromatograms. DryLab®
can select a predefined default string for objects created by DryLab. Ask for a free demo today. More information online:
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Empower Automation also takes into account Audit Trail settings. Depending on the type of Audit Trail (none, restricted or unrestricted), either a general comment string can be used or the user
55324pr@reply-direct.com New SPE Filter for Faster Sample Loading
GL Sciences has introduced a new SPE filter, the EZ Cartridge RP-1. Using this filter, the sample loading time is much faster compared to conventional cartridges.
Using a combination of PTFE fibres and our RP-1 polymer (methacrylate-divinylbenzene), a 0.5mm thick layer is created. Due to this thin layer, sample loading times are 5 times faster. The particle diameter is only 10µm, creating a large surface area for the analyte to interact with. While having a very thin layer, the small particle size results in the analyte to not break through the SPE layer.
EZ Cartridge RP-1 can best be used in the analysis of pesticides in water. the flow rate of a sample through a typical SPE cartridge is 10-20 mL/min, whereas the EZ cartridge can be used with speeds of 100 mL/min. It will show sufficient recovery of the compounds of interest.
EZ Cartridge RP-1 is a hydrophobic SPE cartridge, in functionality comparable with the well-known HLB cartridges. It is ideal for working with watery samples, whether small or large quantities are available. For more information and the pesticide application, please visit the website.
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INTERNATIONAL LABMATE - JULY 2022
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