Environmental Analysis & Electrochemistry
Legionella pneumophila Test Published as ASTM International Standard
ASTM International is an international standards organisation that develops and publishes standards applicable to a wide variety of industries. There are over 12,500 global ASTM standards, developed and reviewed by over 30,000 technical experts from 140 participating countries.
Global water microbiology specialist, IDEXX, has announced that the Legiolert® test, for the rapid detection of Legionella pneumophila, has been published by ASTM International as ‘ASTM D8429-21: Standard Test Method for Legionella pneumophila in Water Samples Using Legiolert’.
This latest standard inclusion follows Legiolert having been specified as a recommended method in the by the UK’s Standing Committee of Analysts’ ‘blue book’ publication, ‘The determination of Legionella bacteria in waters and other environmental samples (2020) - Part 2 - Culture Methods for their detection and enumeration’. Legiolert is also recognised with AFNOR certification for hot and cold sanitary water and cooling tower water (Reference No IDX 33/06 06/19).
A liquid culture method, Legiolert was launched in Europe and North America by IDEXX in 2017 as a highly sensitive method for the confirmed detection of Legionella pneumophila (the primary cause of Legionnaires’ disease) in water, delivering results up to seven days faster than traditional ISO culture testing methods.
There are more than 10 peer-reviewed papers that have highlighted the superior performance of Legiolert when compared to the standard method for detection of Legionella pneumophila. The ISO 11731 method has a number of limitations including a significant false negative error rate which can result in undetected contamination and consequent risk to human health. In contrast, Legiolert is a culture method which is both accurate and easy to use.
More information online:
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QuPPe Method for LC-MS/MS Analysis of Polar Pesticides
The European Union Reference Laboratories (EURL) provide guidance to food safety labs around the world. That guidance can take the form of published methods that help labs looking to develop their own analytical tests, which can be particularly important for challenging analyses like monitoring highly polar pesticides in food. To face this challenge, EURL for Single Residue Methods (EURL-SRM) has published, and continues to update, the Quick Polar Pesticides Method (QuPPe).
Version 12 of the QuPPe-PO Method for products of plant origin includes an approach using Restek’s Raptor Polar X column for the analysis of a wide range of polar pesticides using LC-MS/MS in negative ESI mode.
Restek is proud to have its Raptor Polar X column undergo the significant evaluation conducted by EURL-SRM and then be included in this collection of methods, which are aimed at protecting consumers from harmful pesticides in food. The method can be accessed here:
ilmt.co/PL/ORyk
The Raptor Polar X LC column was specifically designed to address the challenges of LC-MS/MS analyses of polar compounds. It provides excellent retention and separation of polar compounds through its unique hybrid phase chemistry, offering the best of HILIC and ion-exchange retention mechanisms, while still equilibrating quickly and using MS-friendly mobile phases.
Learn more about the Raptor Polar X column or contact your local Restek representative today. More information online:
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Aluminium Monitoring for Wastewater Treatment
Aluminium salts, such as aluminium sulphate and polyaluminium chloride (PAC), are used as coagulation agent and flocculant for treatment of overloaded wastewater. For efficient wastewater treatment, the knowledge of the exact amount of aluminium, expressed as aluminium oxide (Al2O3) in the flocculant, is essential. This can be achieved rapidly and reliably by thermometric titration of the aluminium with sodium fluoride according to the newly revised standard ABNT NBR 11176. To learn more about this analysis, download the Application Note AN-T-224 from Metrohm.
Aluminium has a toxic effect on fish in acidic water and must therefore be removed before the release of the treated water into the environment. The European Union stipulates the monitoring of aluminium as an indicator parameter with a maximum content of 200 µg/L. Adsorptive stripping voltammetry (AdSV) allows the measurement of concentrations as low as 5 µg/L. Compared to atomic absorption spectroscopy (AAS) or Inductively Coupled Plasma Atomic Emission Spectrometry (ICP-AES), voltammetry is a viable alternative with only moderate investment in hardware required and low running costs. Download the Application Bulletin AB-131 from Metrohm to learn more.
More information online:
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