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Advanced Chromatography Modelling and Optimisation Software S-Matrix Corporation, the developer of the Fusion QbD®


Software Platform, announces new automated peak tracking features and capabilities to support Forced Degradation


Studies. S-Matrix was the fi rst company to develop closed-loop experiment automation for Chromatography Data Software (CDS) with integrated Design of Experiments (DoE) methodology for LC method development and validation. S-Matrix was also the fi rst company to integrate true Monte Carlo robustness simulation into chromatographic method development and optimisation software.


S-Matrix has now coupled its powerful PeakTracker™ technology for automated UV and MS spectra based peak tracking with a fully automated workfl ow for Forced Degradation Studies (FDS) within Fusion QbD. To engage the FDS feature you simply set up your method development experiment as usual, and then enter the degradation paths which yielded peaks in the simple setup window pictured in the image shown. Note that in this example only the Oxidation path yielded degradant peaks. As also shown, Fusion QbD will automatically replicate each experiment run to accommodate the compound mix sample (the sample containing all expected peaks) and the additional degradation path sample(s). It will then automatically assign a separate autosampler vial position to each replicate injection when exporting the design to the CDS.


Once the experiment is run in the CDS, Fusion QbD will automatically import the results for each peak in each replicate injection for each experiment run. PeakTracker will then track all peaks in all replicate chromatograms for each run. Once the peaks are tracked, PeakTracker will generate a composite chromatogram for each run containing all unique peaks from the sample compound mix injection and from the replicate degradant path injections. All peak results data for each included peak are automatically aggregated into the peak data set for the composite chromatogram generated for each experiment run, and this data set is then used for the rest of the experiment workfl ow, including data modelling, robust method optimisation, Method Operable Design Region (MODR) visualisation, and chromatogram simulation.


More information online: ilmt.co/PL/RWWO and ilmt.co/PL/Yggp 57465pr@reply-direct.com Versatile Portable GC


The portable GC 312 from PID Analyzers can be utilised for environmental, quality control, process & stack gas monitoring, natural gas, building & transportation security, arson & forensic applications.


Designed in its own carry case, the 312 will go anywhere with its light weight and rechargeable battery which lasts 8 hours. The 312 is pictured in the boot with an optional concentrator to achieve trace level analyses of a particular species, it has signifi cant data storage capability in a 2 GByte smart card, and a choice of excellent GC detectors. The system can accommodate up to two detectors and additional detectors can be added and easily interchanged by the user. This GC features a large colour display and is powered by a Pentium PC with Windows®


10 (embedded) operating system and onboard PeakWorks Chromatography Integration Software.


The principle of operation is gas chromatography with a series of detectors that can be easily interchanged allowing the GC to be used for a variety of applications in the fi eld or the lab. The detector electronics are compact and modular and the detector can be changed by removing the column, connector and two screws. There are only three sets of electronics for the four detectors, so an upgrade only requires the purchase of a second or third detector and second electronics package. PeakWorks is a dual channel system. The GC will accommodate packed (1/8” or 1/16”), capillary (0.53 or 0.32 m id) or PLOT columns by any manufacturer on a 3.5: diameter spool) and an optional heated injector is used for liquid samples. The algorithms for data processing were taken from our powerful PeakWorks program and are incorporated in the embedded software.


Photoionisation detector (PID): VOCs, aromatics, unsaturated hydrocarbons, sulphur compounds and inorganic gases (hydrogen sulphide, arsine/phosphine, ammonia). It is an ideal detector for environmental analysis, and QC with its low ppb to % detection range; also fenceline, water, soil, fi eld measurements.


Thermal Conductivity Detector (TCD): Hydrocarbons, inorganic & fi xed gas response make this detector ideal for applications such as QC, natural gas analysis, LPG analysis, process streams, gas or liquid analysis... in the lab or fi eld- ppm to 100%


Far-Ultraviolet Absorbance Detector (FUV): Hydrocarbons, inorganic & fi xed gas response at levels 10-100 times lower than the TCD- also H2O, O2 ppb to ppm levels


, N2 O at


Flame-Ionisation Detector (FID): Responds to all hydrocarbons including methane- environmental fi eld measurements, landfi ll gases, oil patch monitoring. More information online: ilmt.co/PL/ZkAm


57921pr@reply-direct.com


Robust and Reproducible Sample Preparation for LC/MS


A first choice for LCMS when


ANALYZING POLAR COMPOUNDS Better than HILIC for


Amino Acids, Organic Acids, Amines, and other analytes with improved selectivity due to the very low carbon load of the Silica Hydride.


• Use pH Gradients for separation of closely related compounds.


• Uses Lower Salt Buffers — Protect your LCMS.


• Minimal post time & fast re-equilibration. More throughput.


Visit our website for Application Notes and to learn about more fantastic things you can do with this column.


MANUFACTURED BY: Learn more at: Cogent-HPLC.com 57154pr@reply-direct.com


Porvair Sciences has published an in-depth application report demonstrating how their Microlute® CP Reverse Phase Solid Phase Extraction (SPE) microplates provide a robust and highly reproducible SPE LC-MS sample preparation methodology for a range of neutral, acidic, and basic analytes.


As a sample preparation method, Solid Phase Extraction is widely acknowledged as a ‘go to’ tool for the clean-up of samples before analysis by hyphenated techniques such as LC/ MS or GC/MS. Solid Phase Extraction offers several advantages to the analyst, including less system downtime and troubleshooting, cleaner chromatograms with a reduction of contaminating compounds, and greater reproducible analyte recoveries. Unfortunately, traditional SPE methods rely upon loose-fi lled resins which often create problems such as voids in the sorbent beds leading to channelling and inconsistent fl ow-through of solutions. These undesirable effects lead to reduced interactions between analytes and the active resin resulting in inconsistent results and poor analyte recovery.


Designed to overcome these shortfalls, Microlute® CP SPE microplates from Porvair Sciences, incorporate a unique, polymer


structure made up of an interconnected network of evenly distributed pores combined with retentive media. This hybrid technology ensures even liquid fl ow rates throughout the SPE process, which leads to suffi cient time for Van der Waals forces of interaction to take place between the solid phase sorbent and the analytes resulting in a highly reproducible SPE method.


Experimental data is provided in the application report to show how Microlute® CP RP 96 well microplates selectively retain


and elute a wide range of neutral, basic and acidic compounds. The data demonstrate how percentage relative standard deviation values from the Microlute®


extractions is also shown to offer signifi cant benefi ts for the recovery of hydrophobic basic analytes. Read the application report in full: ilmt.co/PL/9pKn More information online: ilmt.co/PL/6pw3


CP are on average signifi cantly lower than using other commercial SPE plates, thereby


producing more reproducible results. In addition, the application notedemonstrates that no analyte is lost in the load step of the SPE process leading to high recovery values for all types of compounds. The Microlute®


CP RP 96 well-plate for solid phase


INTERNATIONAL LABMATE - JULY 2022


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