Results and Discussion
The performance of the ISQ 7610 system was evaluated using sixteen replicate injections of a 10 fg/µL OFN standard in iso-octane. Stable peak area responses were observed with %RSD of <7% as reported in Figure 1. The minimum %RSD calculated using n=8 injections was 5.7, and it was used to derive the IDL that resulted = 1.7 fg on column. Figure 2 shows the overlaid chromatograms of the injections that were used to derive the IDL. To verify the IDL, a standard of OFN at 2 fg was injected. As demonstrated in Figure 3, shows the OFN peak was clearly detected at such low concentration with a peak-to-peak calculated S/N of 4:1.
%RSD=7
5000 4500 4000 3500 3000 2500 2000 1500 1000 500
Figure 1. Absolute peak area responses over 16 injections.
6.5e3 8.0e3
6.5e3 8.0e3
4.5e3 6.0e3
5.5e3 7.0e3
3.5e3 5.0e3
6.5e3 8.0e3
4.5e3 6.0e3
5.5e3 7.0e3
3.5e3 5.0e3
1.5e3 3.0e3
2.5e3 4.0e3
0.0e0 1.0e3
5.0e2 2.0e3
Figure 2. Overlaid chromatograms of eight consecutive injections of OFN at 10 fg on column showing a peak area %RSD=5.7. The calculated IDL is 1.7 fg. 7.5e3 counts
1.5e3 3.0e3
2.5e3 4.0e3
The ISQ 7610 off-axis ion source, the unique s-shaped ion-guide, and the Thermo Scientifi c™ XLXR™ detector allowed for improved instrument performance. The off-axis ion source and unique s-shaped ion-guide creates a curved path, which allows chemical and neutral noise to be kept to a minimum, enabling lower detection limits. The XLXR detector provides the sensitivity to detect and amplify the low number of transmitted ions needed at this challenging analyte concentration.
0.0e0 1.0e3
5.0e2 2.0e3
RT: 3.863 – OFN min
4.5e3 6.0e3
5.5e3 7.0e3
7.5e3 counts
3.5e3 5.0e3
1.5e3 3.0e3
2.5e3 4.0e3
0.0e0 1.0e3
5.0e2 2.0e3
min 7.5e3 counts
5000 4500 4000 3500 3000 2500 2000 1500 1000 500
RT: 3.863 – OFN
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Injection number
RT: 3.863 – OFN
5000 4500 4000 3500 3000 2500 2000 1500 1000 500
%RSD=7
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Injection number
2.6e3 2.5e3 2.4e3 2.3e3 2.1e3 2.0e3 1.9e3 1.8e3 1.6e3 1.5e3 1.4e3 1.3e3 1.1e3 1.0e3 8.8e2 7.5e2 6.3e2 5.0e2 4.0e2
2.8e3 2.8e3
1.6e3 1.5e3 1.4e3 1.3e3 1.1e3 1.0e3 8.8e2 7.5e2 6.3e2 5.0e2 4.0e2
counts
RT: 3.865 – OFN min 19
AS 1610 autosampler Syringe
Injection volume
AS 1610 autosampler Syringe
Pre-injection solvent and cycles None Sample rinses
2.6e3 2.5e3 2.4e3 2.3e3 2.1e3 2.0e3 1.9e3 1.8e3 1.6e3 1.5e3 1.4e3 1.3e3 1.1e3 1.0e3 8.8e2 7.5e2 6.3e2 5.0e2 4.0e2
2.8e3 2.8e3
Injection volume
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Injection number
%RSD=7 Column
Post-injection solvent and cycles None TRACE 1610 GC system
Pre-injection solvent and cycles None Sample rinses
Conclusion ColumnSSL mode Liner
AS 1610 autosampler Syringe
Liner
Injection volume Inlet temperature
Split flowReferences Splitless time
5
Post-injection solvent and cycles None TRACE 1610 GC system
10 µL, 25 gauge, 50 mm length, cone tip (P/N 36500525)
5
Figure 3. Chromatogram of 2 fg OFN on-column with a signal-to-noise ratio of 4 to 1. 1 µL
Thermo Scientific™ TraceGOLD™
The ISQ 7610 system with the ExtractaBrite ion source exhibits low instrument detection limits, ensuring confi dence in trace analysis. The use of a statistical method to determine the IDL demonstrates the consistent and sensitive detection that can be achieved at this challenging analyte concentration. The detection limits using a 10 fg on-column amount have been shown to be highly reproducible. The exceptionally sensitive XLXR detector, complemented by the innovative s-shaped ion guide, effectively reduces noise while maintaining maximum sensitivity, leading to OFN IDLs signifi cantly below 5 fg on column.
TG-5MS 30 m × 0.25 mm × 0.25 µm (P/N 26098-1420)
TG-5MS 30 m × 0.25 mm × 0.25 µm (P/N 26098-1420) 220 ˚C
Thermo Scientific™ TraceGOLD™ Splitless
1 µL
Pre-injection solvent and cycles None Sample rinses
5 SSL mode
Split flow Column
Liner
Inlet temperature Carrier flow
Splitless time
Post-injection solvent and cycles None TRACE 1610 GC system
Septum purge flow Oven program
2. International Union of Pure and Applied Chemists (IUPAC),
http://media.iupac.org/publications/analytical_ compendium/Cha18sec437.pdf
Splitless 220 ˚C
Constant flow of 5.0 mL/min Constant He flow of 1.2 mL/min
To request a technical note titled Determination of the instrument detection limit of the TSQ 9610 triple quadrupole GC-MS/MS with Advanced Electron Ionization (AEI) source, please contact: CMD.APACMarketing@thermofi
sher.com
50 mL/min 0.5 min
Septum purge flow Find out more at thermofi
sher.com/ISQ7610 Carrier flow
ISQ 7610 GC-MS system MS transfer line temperature OvIeon source temperature Ionization mode
n pr gram SSL mode
Mobile NIR Spectroscopy Solution for Animal Feed AnalysisDetector gain Oven program
Carrier flow
A portable NIR solution that provides customers with a fast and reliable on-site analysis of animal feed and ingredients has been announced by BASF Animal Nutrition and trinamiX GmbH.
min Untitled-5 3
By signifi cantly miniaturising conventional NIR spectroscopy, the trinamiX’ Mobile Near Infrared (NIR) Spectroscopy Solution provides a high-performance ‘lab in the pocket’. Together with BASF, the company is now using their solution to accelerate and simplify feed analysis. The collaboration brings together BASF’s animal nutrition knowledge and trinamiX’ expertise in mobile NIR spectroscopy. The result is a powerful, cloud-based combination of a handheld device, smartphone app, and customer portal which enables customers to receive relevant insights into the quality and nutritional value of samples at the push of a button. The solution thus enhances feed formulation and quality control. to address the industry’s most complex challenges.
ISQ 7610 GC-MS system MS transfer line temperature Ion source temperature Ionization mode Emission current Scan start Scan mode Dwell time
Inlet temperature Emission current
Split flow Scan start
Splitless time Scan mode
Septum purge flow Dwell time
Detector gain
The new NIR solution covers a broad variety of samples, ranging from feed ingredients to fi nished feed to forage. It supports parameters that are crucial to feed quality and the wellbeing of livestock, including moisture, protein, fat, and energy.
Untitled-5 3
Measuring a sample takes less than a minute and can be performed on the spot. The results are instantly displayed in the smartphone app and are permanently accessible via trinamiX’ web-based customer portal for further evaluation.
Using our mobile NIR solution, feed millers, nutritionists, and farmers get a fast and independent solution to guarantee the quality of animal diets,” said Manuel Rez, Director Product Management Performance Ingredients & Marketing at BASF Animal Nutrition.
“By combining accurate results with the fl exibility of a portable device which fi ts into your pocket, we are paving the way for an effi cient and reliable approach to feeding,” explained Nils Mohmeyer, Head of Business Development and Sales Spectroscopy at trinamiX. “Our customers no longer need to send samples to the laboratory. Instead, the laboratory comes to them,” he added.
More information online:
ilmt.co/PL/4pBB 57091pr@reply-direct.com
ISQ 7610 GC-MS system MS transfer line temperature Ion source temperature Ionization mode Emission current Scan start Scan mode Dwell time
Detector gain
SIM m/z 272 0.1 s
1.00E+06 3.10 min Thermo Scientific™ TraceGOLD™
Constant flow of 5.0 mL/min Constant He flow of 1.2 mL/min
45 ˚C (0.5 min), 40 ˚C/min to 190 ˚C (0 min)
250 ˚C 200 ˚C
Splitless EI
Read, Share and Comment on this Article, visit:
www.labmate-online.com/article
250 ˚C 200 ˚C EI
50 µA
220 ˚C 50 µA
50 mL/min 3.10 min
0.5 min SIM m/z 272
Constant flow of 5.0 mL/min 0.1 s
3.10 min1.00E+06 SIM m/z 272 0.1 s
1.00E+06
250 ˚C 200 ˚C EI
50 µA Constant He flow of 1.2 mL/min
45 ˚C (0.5 min), 40 ˚C/min to 190 ˚C (0 min)
TG-5MS 30 m × 0.25 mm × 0.25 µm (P/N 26098-1420)
45 ˚C (0.5 min), 40 ˚C/min to 190 ˚C (0 min)
Splitless Liner single taper with wool, 4 mm ID, 78.5 mm length (P/N 453A0924)
1. U.S. Code of Federal Regulations, 49 FR 43430, (Oct. 26, 1984); 50 FR 694, 696 (Jan. 4, 1985), as amended at 51 FR 23703 (June 30, 1986),
http://www.ecfr.gov/cgi-bin/text-idx?SID=efe93db42854f88dffcf 66ba8de737e6&mc=true&node=ap40.23.136_17.b&rgn=div9
Splitless Liner single taper with wool, 4 mm ID, 78.5 mm length (P/N 453A0924)
50 mL/min 0.5 min
10 µL, 25 gauge, 50 mm length, cone tip (P/N 36500525)
Splitless Liner single taper with wool, 4 mm ID, 78.5 mm length (P/N 453A0924)
counts
RT: 3.865 – OFN min
10 µL, 25 gauge, 50 mm length, cone tip (P/N 36500525)
1 µL min
08/03/2022 10:41
08/03/2022 10:41
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WWW.LABMATE-ONLINE.COM
08/03/2022 10:41
3.80 3.81 3.82 3.80 3.80 3.81 3.82 3.80 3.81 3.82 3.83 3.84 3.85 3.80 3.81 3.82 3.83 3.84 3.85 3.86 3.87 3.88 3.89 3.90 3.91 3.92 3.93 3.86 3.87 3.88 3.89 3.90 3.91 3.92 3.93 3.83 3.84 3.85 3.86 3.87 3.88 3.89 3.90 3.91 3.92 3.93 3.80 3.81 3.82 3.83 3.84 3.85 3.86 3.87
3.88 3.89 3.90 3.91
3.92 3.93
3.81 3.82 3.83 3.84 3.85 3.86 3.87
3.88 3.89 3.90 3.91
3.83 3.84 3.85 3.86 3.87
3.88 3.89 3.90 3.91
3.92 3.93
3.92 3.93
Peak area, counts*s
Peak area, counts*s
Peak area, counts*s
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