12 Discover the Future Safety Solution for HPLC Waste
Discover the liquid safety solution of the future, and turn your waste container into a closed safety system. The modular Safety Waste Cap ‘LISA’ blocks chemical vapours with its 3-layered exhaust air filter. It connects safely to your HPLC waste lines, and provides safe disposal of chemicals like solvents, acids and alkalis. It comes with multiple connectors for many tubing sizes, and is available for different containers and thread types.
The Safety Waste Cap is made of chemically resistant materials (PTFE and PE-HD) for use with all common chemicals used in analytical labs. The modular system is extendable with accessory ‘Satellites’ for additional connections and applications.
In order to block hazardous vapours and avoid overpressure inside the container, the Safety Waste Cap LISA works perfectly with the original 3-layered SCAT exhaust air filters. The filters contain 3 types of activated carbon, optimised for catching vapours of solvents, acids and alkalis.
The screw cap is freely rotatable and prevents your HPLC tubing from twisting when changing the container. Due to the variety of included connectors for different tubing sizes, LISA fits every HPLC/UHPLC system. To keep the system safely closed, it comes with blind plugs for every connection port.
For use in explosion-protected zones, electrically conductive versions are available. They provide a grounding connection for antistatic protection, and are designed for use with electroconductive containers.
More information online:
ilmt.co/PL/x99n and
ilmt.co/PL/Ly2P 57784pr@reply-direct.com
Maximum flexibility with YMC-Triart Determination of Oxygenates and BTEX in Beverage Grade Carbon Dioxide by GC
The following gas chromatography (GC) method was developed for the simultaneous analysis of ppb levels of the oxygenates acetaldehyde, dimethyl ether (DME) and benzene, toluene, ethylene and xylene (BTEX) in beverage grade carbon dioxide by installing a second column to separate acetaldehyde and DME from the carbon dioxide and the BTEX. A four port switching valve was used for column selection.
This GC method was performed on a process analyser configured with a photoionisation detector which has a detection limit for BTEX with no preconcentration at < 0.5 ppb; acetaldehyde at <50 ppb and DME at < 20 ppb. The analysis was automated via a programmable logic controller (PLC) that controls the analyser (start, calibrate, send/ print report). The embedded software operating system is Windows® 10 which utilises PeakWorks, a chromatography integration system for peak identification and quantification. Data was generated that yielded the following results: <100 ppb (acetaldehyde), <50 ppb (dimethyl ether), and 5 ppb BTEX for a direct injection with no concentration of the sample. The analysis time for this multicomponent analysis was < 10 minutes. This system can be remotely operated.
Contact PID Analyzers to request a quotation or learn more. More information online:
ilmt.co/PL/VORd
57924pr@reply-direct.com
Synthetic oligonucleotides are extremely promising candidates for biopharmaceuticals in a wide range of diseases. This is why nucleic acid therapeutics such as silencing RNA (siRNA), messenger RNA (mRNA) and antisense oligonucleotides (ASOs) are gaining considerable attention in current research.
Phosphoramidites are the essential part of the chemical synthesis of oligonucleotides, short fragments of nucleotides and analogues. To prevent any side reactions on residual reactive sites such as hydroxyl and amino groups during oligonucleotide synthesis, these groups must be protected. Therefore, the highly reactive native nucleotides are modified with four different protecting groups. Figure 1 shows the protecting groups using the example of an adenosine based phosphoramidite.
During the chemical oligonucleotide synthesis, errors can occur which is why the purity of the phosphoramidites needs to be closely monitored. In a new application note two different separation modes - reversed phase (RP) and normal phase (NP) - are used for the analysis of four different phosphoramidites. Due to the complexity of these molecules different interactions can be used in order to achieve reasonable retention and resolution. As phosphoramidites have a chiral centre at the phosphorus atom two isomers are present resulting in double peaks occurring in the chromatograms.
Preparat ive HPLC System
•Quick and Simple Method Development •Flow Rates from 1 to 150 mL/min •Operating Pressure Up to 6,000 psi
•Built-in Features Take Up Less Precious Bench Space
All separations were performed using the highly robust YMC-Triart columns which are an ideal choice for modern biochromatography applications. For RP separations a YMC-Triart C18 column was used resulting in sharp peaks. A YMC-Triart SIL a mobile phase containing triethylamine as additive (examples in Figure 2). Excellent resolution was obtained for all compounds.
Read the application note:
ilmt.co/PL/j4KV More information online:
ilmt.co/PL/RyR1
57700pr@reply-direct.com
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HPLC Analysis of Phosphoramidites using RP or NP Conditions
INTERNATIONAL LABMATE - JULY 2022
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