45 Proteomics, Genomics & Microarrays Aptamers for Cancer Diagnosis and Therapy


There are two major applications for aptamers in cancer therapy. Aptamers can be used as antagonists by targeting and inhibiting cancer- specific molecules. They can also be used as delivery vehicles for therapeutics by specifically targeting cell membrane receptors on cancer cells. In this application, aptamer-drug conjugates bind to and are internalised by cancer cells while avoiding damage to healthy cells.


AMSBIO offer an extensive range of aptamers targeting many human proteins linked to cancer. Aptamers bind to target molecules with the same affinity and specificity as antibodies but offer significant advantages, in particular a lack of immunogenicity, which makes them an ideal tool for cancer diagnosis and therapy.


Aptamers also have enormous potential for use in cancer diagnostics. They can be used to identify cancer cells and recognise cancer biomarkers, cancer metabolites and differentiating cells as well as molecules that affect tumour behaviour. New aptamer-based technologies such as cell-SLEX are increasingly being used to screen for aptamers that bind to cancer cell-surface protein biomarkers as they overcome the traditional shortfalls of mass spectrometry and antibody-based methods - cross-reactivity, poor reproducibility, complexity, and high cost.


More information online: ilmt.co/PL/lBlk 57965pr@reply-direct.com


New Gene Tagging Kits


Invitrogen TrueTag Donor DNA Kits from Thermo Fisher Scientific simplify and accelerate cell line engineering with CRISPR-Cas9 or TALEN technology. Researchers can now enrich tagged cells to obtain up to 100% of edited cells, enabling them to skip the time-consuming clonal isolation step and save up to four months on their genome editing experiments, when compared to traditional gene tagging workflows. These off-the-shelf kits provide templates and clean-up columns to generate donor DNA with a one-step PCR workflow which eliminates the need for cloning.


The expanded portfolio of TrueTag Donor DNA Kits supports four types of locus-specific knock-in templates: Fluorescent tagging kits; Epitope tagging kits; Stem cell fluorescent reporter tagging kits; Knockout enrichment kits.


TrueTag Donor DNA Kits are offered for fluorescent and epitope tags including GFP, RFP, BFP, Myc, HA, 6xHis, YFP and DDK.


Applications include genome editing experiments including creating cell lines for disease model studies and reporter assays, tagging genes for localisation and isolation studies and enriching cells with functional gene knockout. More information online: ilmt.co/PL/km5r


57973pr@reply-direct.com


High Performance Microplates Enhance Cell Growth and Survival


The Sero Krystal™ PDL range of coated microplates from Porvair Sciences set a new benchmark for reproducible cell culture even with challenging cell types. Many scientists would prefer to grow cells in a serum-free environment to more accurately mimic physiological conditions. However, this often presents challenges as cell lines often struggle to attach to traditional tissue culture plastics in the absence of serum. Manufactured from ultra-pure polystyrene in a clean room environment, Sero Krystal™ Poly-D-Lysine (PDL) coated microplates were developed by Porvair Sciences to overcome these challenges and promote superior cell attachment, growth and proliferation for a wide range of cell types in serum-free media.


Poly-D-Lysine is a synthetic (animal-free) extracellular matrix used in cell culture applications to promote cell adhesion. Available in 96- and 384-well formats these new Sero Krystal™ PDL microplates are uniformly coated with a 70 to 150 kDa PDL polymer using proprietary methods. The coating creates a uniform net positive charge on the surface of the plate wells. This positive surface charge improves electrostatic interactions between the coated surface and the negative charges of the cell membrane resulting in improved cell attachment. Sero Krystal™ microplates are ideal for the culture of transfected cell lines, primary neurons, glial cells and fibroblasts, such as the adherent mouse fibroblast cell line L929 and other common adherent cell lines including HEK-293, BHK-21, PC12 and, NSC-34.


Available in clear, black and frame versions - Sero Krystal™ PDL coated microplates incorporate an optically clear base. The consistent flatness of the Sero Krystal™ microplate base gives superior light transmission for high resolution imaging and a flat optical plane for growing cells.


Supplied sterile and ready-to-use, Sero Krystal™ PDL coated microplates are the first part of the new Sero™ range of cell culture plates to launch from Porvair Sciences. More information online: ilmt.co/PL/kmZm


57687pr@reply-direct.com


Game-changing Platform Automates Genomics Liquid Handling


The firefly from SPT Labtech is an innovative liquid handling platform for next-generation sequencing (NGS) library preparation. firefly transforms NGS liquid handling workflows by bringing together pipetting and dispensing within a compact all-in-one design. Along with its hardware capabilities, the platform sets a new standard in powerful, intuitive liquid handling software with multiple user levels, intelligent programming and a fully transparent audit trail. A complementary peer-to-peer cloud-based network- the first of its kind- will allow researchers to share their custom protocols, as well as benefit from SPT Labtech’s validated tools to underpin scientific collaboration and innovation. The launch of firefly builds on SPT Labtech’s strong track record in engineering solutions for genomics applications.


Paul Lomax, Genomics Product Manager at SPT Labtech said: “While advances in next-generation sequencing have revolutionised the genomics field, library preparation methods remain a significant bottleneck for many laboratories. Liquid handling automation offers substantial throughput advantages, overcoming the need for multiple instruments, significant lab space, and high levels of investment. With its all-in-one capabilities, compact design and innovative software, firefly offers a step-change in productivity for genomics laboratories- at an affordable entry point.”


firefly comes application-ready for library preparation kits NEBNext Ultra II DNA; NEBNext Ultra II FS DNA; NEBNext Ultra II RNA, and Illumina DNA Prep with an extended range already under active development.


David Newble, CEO, at SPT Labtech, added: “Our team applies ingenuity to overcome customers’ workflow challenges and accelerate research. We are excited about the potential of the accessible, cost-effective firefly instrument to further democratise the adoption of liquid handling automation for NGS applications. Collaboration is a cornerstone of innovation, and by enabling peer-to-peer knowledge sharing, we aim to empower bigger genomics breakthroughs and advance health.”


More information online: ilmt.co/PL/ZZma 57232pr@reply-direct.com


Page 1  |  Page 2  |  Page 3  |  Page 4  |  Page 5  |  Page 6  |  Page 7  |  Page 8  |  Page 9  |  Page 10  |  Page 11  |  Page 12  |  Page 13  |  Page 14  |  Page 15  |  Page 16  |  Page 17  |  Page 18  |  Page 19  |  Page 20  |  Page 21  |  Page 22  |  Page 23  |  Page 24  |  Page 25  |  Page 26  |  Page 27  |  Page 28  |  Page 29  |  Page 30  |  Page 31  |  Page 32  |  Page 33  |  Page 34  |  Page 35  |  Page 36  |  Page 37  |  Page 38  |  Page 39  |  Page 40  |  Page 41  |  Page 42  |  Page 43  |  Page 44  |  Page 45  |  Page 46  |  Page 47  |  Page 48  |  Page 49  |  Page 50  |  Page 51  |  Page 52  |  Page 53  |  Page 54  |  Page 55  |  Page 56  |  Page 57  |  Page 58  |  Page 59  |  Page 60  |  Page 61  |  Page 62  |  Page 63  |  Page 64  |  Page 65  |  Page 66  |  Page 67  |  Page 68