61 New Technology Upgrades the Spatial Resolution of Two-photon Excitation Fluorescence Microscopy
Ideal for high-precision observations of deep regions in biological samples, this technology from Hamamatsu Photonics will become an indispensable tool for understanding brain functions. This was achieved through intensive research efforts using the company’s spatial light modulators (SLM) and their unique optical control technology fostered over many years.
Applying results from this research will easily enhance the resolution of two-photon excitation microscopes equipped with an SLM. This new microscopy technique is being put to practical use across a broad range of fi elds including neuroscience and biology. It allows for detailed measurements of deep regions in biological samples and highly accurate observations of changes in the state of organelles that make up cells. This makes it a promising tool for applications in research on brain functions, kidney disease and other illnesses.
The results from this research were published on 20 April in the leading scientifi c journal on neuroscience ‘Frontiers in Neuroscience’. Results were achieved by a joint research effort with a laboratory endowed for Biophotonics Innovation and from a course on Virology and Parasitology offered at the Hamamatsu University School of Medicine.
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Digital Microscope Camera for Standard Brightfi eld Imaging
The LC35 microscope camera makes capturing quality brightfi eld images affordable. With a 3.5-megapixel CMOS sensor and versatile exposure times and resolution modes, the camera’s provides quality brightfi eld images in a cost-effective package.
Flexible for use with a wide range of samples under different observation conditions, the LC35 camera delivers the detail needed for routine imaging, inspection and documentation. The frame rate of up to 40 frames per second (fps) makes navigating around the sample fast to achieve precise focusing and fast image capture.
The camera integrates seamlessly with Olympus life science and industrial imaging systems, including full microscope set up assistance, software support and optical compatibility. For basic imaging and documentation, customers can use the free LCmicro software, while advanced image analysis is possible using cellSens™ software for life science and Olympus Stream™ and PRECiV™ software for industrial applications.
Installing the camera is simple - a single USB 3.1 cord provides power and a fast connection to your PC or laptop. The camera easily mounts to any light microscope using different C-mount adaptors. Using the camera is just as easy. With only a little training, operators can use the camera to its full potential, including interactive measurements, commenting on images, archiving and creating reports.
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Optimised Antibody Labelling Kits for Super-Resolution Microscopy
The Mix-n-Stain™ labelling kit line from Biotium has been expanded with new kits designed specifi cally for producing antibody conjugates ideal for super-resolution stochastic optical reconstruction microscopy (STORM). Mix-n-Stain™ STORM CF® Biotium’s unique STORM CF®
kits are optimised to yield a low degree of labelling that is optimal for single molecule localisation microscopy techniques such as STORM.
STORM image quality is highly dependent on the properties of both the fl uorophore and antibody conjugate. Specifi cally, antibodies conjugated to bright fl uorophores with robust photo-switching properties at a low degree of labelling are ideal for STORM. However, a convenient method to produce antibody conjugates with a low degree of labelling for STORM is lacking. Moreover, while several red-excited dyes suitable for STORM are available (e.g., AF647, CF®
647, CF® 660C, and CF® With the release of Mix-n-Stain™ STORM CF® near-IR. CF® 583R and CF®
producing antibody conjugates optimal for STORM imaging in a variety of colours. Mix-n-Stain™ STORM CF®
Berkeley Professor and STORM scientist Dr Ke Xu, PhD. More Information online:
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Dye Antibody Labelling Kits are available with 8 STORM-validated CF® 597R are novel green-excited CF®
Dyes with emission ranging from visible red to Dyes for multi-colour STORM that were recently published in collaboration with UC Dye Antibody Labelling Kits are available with Dyes, which feature exceptional brightness and photochemical properties ideal for STORM imaging. In addition, the
680), there are limited options for green-excited fl uorescent probes for multi-colour STORM. Dye Antibody Labelling Kits, Biotium has met this challenge by offering a streamlined solution for
New Platform Provides Integrated Imaging Solution
A new platform from Leica Microsystems offers a new type of wholly integrated imaging solution that combines widefi eld and confocal imaging in a single system, while also leveraging machine learning software, automation tools and unique fl uorescence unmixing techniques to automate imaging workfl ows.
Mica, the world’s fi rst Microhub, allows researchers, regardless of expertise, to work in a single digital imaging platform, moving confi dently from setup to beautifully visualised results, allowing true access for all. The Microhub intelligently automates sample-fi nding, parameter-setting and focus constancy, replacing manual setup with just one push of a button. Mica eliminates over 85% of the tedious setup steps in the conventional imaging workfl ow.
Mica also fully integrates everything a researcher needs for radically simplifi ed workfl ows, using automation and AI to enable deeper understanding and a faster track to publication. For example, to perform a complex experiment such as a fl uorescence multi-well plate assay, the current workfl ow can be simplifi ed from 24 steps using a conventional microscope to just 8 steps with the use of Mica.
“Research paradigms are constantly evolving, as we have seen with the recent evolution of single cell and transcriptomics. The next frontier is about essentially putting this dissociated information into spatial context, as location is critical to understanding biological mechanisms. The trend is driving more researchers to utilize complex imaging in their research. We have built Mica for these researchers, who need to focus more on their biology than the specialism of microscopy,” said James O’Brien, Vice President Life Sciences at Leica Microsystems. “The Microhub era will supercharge the microscopy experience for everyone.”
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