Sample Preparation & Processing
Sample Preparation for Drug testing via Hair Analysis
New Knife Mill Provides Quick and Effective Sample Homogenisation
Tough? Fatty? Fibrous? No problem for the new GM 200. The GRINDOMIX is the perfect mill to homogenise dry, oily, fatty, soft and tough sample materials - for analysis results with minimum standard deviation.
The model GM 200 for sample volumes up to 700 ml has been completely revised. Thanks to the powerful 1000 W drive and the innovative Boost function with 14.000 rpm, the mill is powerful enough to homogenise even difficult samples such as tough meat with skin or fibrous plants very quickly and efficiently.
The new GM 200 is operated via a user-friendly 4.3’’ touch display and allows storage of 8 SOPs. A QR code in the display leads the user to the MyRETSCH web portal which provides a wealth of product- and application-related information.
The GM 200 is one of the first mills to be controlled via the optional Retsch
App which allows for creating your own application routines, accesses the Retsch application database and facilitates direct contact to the service team.
More information online:
ilmt.co/PL/M10G 48382pr@reply-direct.com
In order to prove the consumption of drugs (cocaine, cannabis products, amphetamines) even after long periods of time, hair is frequently analysed.
It is dissolved in certain acids and then analysed via gas chromatography or the mass spectrometer. The dissolving in the acid is accelerated and improved through the enhancement of the specific surface area of the sample. Here the Fritsch Mini-Mill -PULVERISETTE 23 is used.
In a 15ml steel cup, 300-500mg hair is weighed. As a grinding body a 15mm steel ball is used. After a grinding duration of 5 minutes a fine powder is attained (< 100µm), which can be used for the following analysis.
For larger amounts or for the grinding of two samples simultaneously, the Fritsch Planetary Micro Mill PULVERISETTE 7 classic line can be used. In a 45 ml grinding bowl made of zirconium oxide, sintered corundum, silicon nitride approximately 0.1-1g of hair is weighed in and 7x15mm grinding balls are added. The grinding time is 5 - 20 minutes. Afterwards a fine powder is obtained (< 100 µm), which can be used for additional analysis.
More information online:
ilmt.co/PL/gnEJ 50004pr@reply-direct.com High Throughput Sample Preparation for Toy Testing
Children’s toys and jewellery products sold in the EU are legislated to meet requirements stated in CPSC, ASTM and EN standard test methods. These methods specifically require a microwave digestion system to prepare the samples for the subsequent determination of lead and cadmium by ICP.
The requirement for total dissolution presents accredited testing laboratories and the analyst with unique challenges as high temperatures and pressures can be required to fully digest certain samples. In addition, the variety of materials often means that samples have to be processed in small batches due to differences that can occur when they are prepared in a traditional microwave. This can cause a bottle neck in the laboratory workflow and can extend turnaround times to many days.
The Milestone UltraWAVE sample digestion system from Analytix allows digestion of up to 15 different samples with different sample weights using different acids all in the same digestion run so there is no need to batch samples of the same type into separate runs. At the heart of the system is a large sample chamber that offers digestion of larger sample amounts using less acid and even weaker acids than previously used thus increasing the sensitivity of AA or ICP analysis.
Very little method development is required with high throughput being achieved through extremely fast heating coupled with a cooled chamber that can reduce total run times to as low as 20 minutes. Operation is so simple that all you need to do is weigh your sample into a disposable low cost vial, load it onto the support rack and press ‘START’, there is no lengthy capping and uncapping procedure required.
With a top temperature of 300°C and the capability to operate at pressures up to 199 bar, even the most difficult samples can be digested. This makes the UltraWAVE perfect for toy testing laboratories that have multiple sample types to digest or want the high throughput capability of SRC technology.
More information online:
ilmt.co/PL/yKmB 51150pr@reply-direct.com Pipetting Solutions for Reproducibly Improving Magnetic Bead DNA Clean-up
Magnetic beads are an efficient way to clean up nucleic acid samples, but most protocols involve many wash and transfer steps, which can be tedious and time consuming to carry out manually. Integra Biosciences’ innovative pipetting solutions are the perfect partner for magnetic purification protocols, allowing the user to reproducibly obtain high yields for a variety of downstream applications - including PCR, NGS, Sanger sequencing, immunoprecipitation, cloning and microarrays.
Integra offers a range of pipetting solutions to support maximum sample recovery with magnetic beads. The ASSIST PLUS pipetting robot and VIAFLO 96/384 handheld electronic pipette help the user to perform multistep protocols quickly, efficiently and reproducibly, and both benefit from unmatched ergonomics, eliminating the risk of repetitive strain injuries associated with intensive mixing steps and repeated tip ejection.
The VIAFLO 96/384 is perfectly suited to sample clean-up, allowing an entire 96 or 384 well plate to be purified in a fraction of the time it would take with a traditional pipette. This affordable, compact and easy-to-use solution increases your throughput and reproducibility, with interchangeable pipetting heads to suit the plate format and volume range of each application.
The ASSIST PLUS pipetting robot automatically carries out reagent transfer, mixing steps and tip changes. This precise and careful handling of magnetic beads leads to superior reproducibility and consistency, as well as ensuring optimal timing for every incubation step. Any of Integra’s 25 electronic multichannel pipettes - including the VOYAGER adjustable tip spacing pipette and the VIAFLO electronic pipette - can be used with the ASSIST PLUS, for complete application flexibility. Programs can be set up in the VIALAB pipette automation software, and the parameters can be easily fine-tuned for consistent results.
These pipetting solutions can also be combined with Integra’s Low Retention GripTips. These work by retaining less liquid compared to standard tips, ensuring that beads and sample are not lost and that dispenses are as accurate as possible.
More information online:
ilmt.co/PL/k0Lj 50960pr@reply-direct.com
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