59 Obtaining Sharper Clearer Images


The Olympus cellSens deconvolution solution module employs a constrained iterative (CI) algorithm to remove out-of-focus blur in advanced life science imaging. This high-speed operation provides extremely sharp images, as well as near confocal quality, with the ability to correct for any spherical aberrations. Integrating seamlessly with the cellSens Dimensions software, outstanding performance and quality are guaranteed.


Using a specialised and improved algorithm developed exclusively for Olympus, the deconvolution solution module provides advanced, high-speed image restoration. Maximising modern PC computing capabilities, it leverages multi-core processor architectures to provide an extremely powerful deconvolution facility, enabling the sharpening of even the most blurred images. Consequently, users can obtain a fast and easy-to-use, comprehensive deconvolution, removing the complex parameter adjustment steps required with other advanced de-blurring algorithms.


The resulting high-quality image stacks can be visualised in three dimensions, using the powerful VoxelViewer to display structures and ISO projections via a simple click of the mouse. This enables more in-depth analysis, with the capability to display stereo adaptations of the image. Furthermore, the online de-blur functionality enables users to visualise the deconvoluted images during the live operation of the camera.


A broad range of image types can be deconvolved using the Olympus deconvolution solution module, including fluorescence, confocal and brightfield. With the additional capability to perform blind deconvolution, the software is able to use a theoretical point spread function (PSF), adapting it to the specific data. Furthermore, the PSF calculation can be saved and applied when performing subsequent deconvolutions. In addition, images obtained from non-Olympus systems are also easily imported, making it a highly versatile imaging solution.


Designed to offer control and processing for microscope-based experimental systems, the cellSens Dimensions software package provides a broad range of advanced features, as well as specialised, optional solution modules.


Circle no. 217


Advanced Fully Digital SPM Control Station


JPK Instruments announces the Vortis™ Advanced, the new standard in SPM Control systems with the lowest noise and highest signal speeds seen to date. Providing the highest performance of electronics controller for scanning probe microscopes, SPM is one of the driving forces behind JPK's development programs. Recent engineering breakthroughs have enabled the elimination of DSP technology. JPK has utilised the new Power-PC hardware with the latest FPGA architecture to deliver the highest digital performance.


Vortis Advanced is now available with all of JPK's SPM systems: the NanoWizard® 3, the ForceRobot® 300 and the CellHesion® 200 now deliver even more


superior results. With fast signal acquisition and control, advanced feedback and analysis are the keys for a modular and ultra flexible controller. The lowest noise levels are achieved using a new grounding concept, intelligent signal conditioning, temperature stabilised oscillators and passive cooling.


Vortis Advanced has been designed so the builder of their home-designed and built SPM heads may utilise these performance benefits. It comes with a large number of user accessible analogue and digital signal channels, fast responding lock-in amplifiers and high-end piezo drivers with closed loop control. Flexibility of design gives the user the ability to work with different probe configurations requiring different levels of control. Synchronisation with external instruments including spectrometers, potentiostats and optical detection devices is provided too.


Control systems with the best hardware still require another vital element, powerful and modular software. JPK supplies the new package, SPMControl v4 to deliver ease of use plus the ability for user customised experiments through the implementation of many powerful data analysis and processing routines such as multi-channel oscilloscope functionality, advanced filtering, batch processing and channel overlay.


Circle no. 218


Adaptable Microscopy Systems Set New Standards in Ergonomics and Efficiency


Olympus has previewed the new BX3 range of upright clinical microscopes featuring the BX43 system microscope and the BX46 ergonomic screening microscope. As with the highly successful BX2 range, the BX3 clinical microscopes incorporate the advanced Olympus UIS2 optical components and are uniquely adaptable, ensuring the ultimate level of imaging flexibility and ergonomic operation. In combination with numerous motorisation and contrast options, intuitive labSens software and the Olympus digital-imaging camera range, the BX3 microscopes are digital documentation and future-lab-ready, enabling your microscope system to adapt in line with your changing and growing workflow requirements.


The novel, true-colour LED illumination system has a unique wavelength profile, which provides a colour-rendering index matching that of a halogen bulb with a daylight filter. Furthermore, the true-colour LED system is controlled by the Light Intensity Manager (LIM), which automatically adjusts the intensity to user-defined levels for each objective change, thus removing the need for manual adjustment and making screening more efficient.


The BX3 microscopes are available with the world’s first tilting/telescopic/lifting observation tube that provides adjustment in three dimensions: eyepiece tilt -3° to +27º, tube extension (backwards/forwards) of 55 mm and lifting of 45 mm.


The BX3 range features coded nosepiece and mirror turret modules, enabling users to automatically record and share microscope magnification and setting information for comparing, measuring and scaling images.


The BX3 range of clinical microscopes from Olympus is ideal for covering all the current clinical requirements and protocols. More importantly, it is also ready for the future, whatever that may bring.


Circle no. 219 Circle no. 220


INTERNATIONAL LABMATE - OCTOBER/NOVEMBER 2010 - MICROSCOPY & MICROTECHNIQUES


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