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Free Demo Software Offer for GPC/SEC Users
CHROMATOGRAPHY & SPECTROSCOPY
New Papers For Phosphopeptide Scientists
Glygen Corporation announce the availability of a large number of downloadable papers from their new enhanced website, which details the wide use their products have been utilised in. Glygen the world leader in micro-sample phosphopeptide purification tools and kits, with recent NIH research (Gates, et al, ‘Comparison of Metal and Metal Oxide Media for Phosphopeptide Enrichment Prior to Mass Spectrometric Analyses’, JASMS June 2010) indicating that, under the experimental conditions used, TiO2 NuTips resulted in the reproducible observation of a greater number of unique sites of phosphorylation with the least amount of nonspecific binding… dramatically improving detection and sequencing of phosphopeptides.
Additionally, recent literature suggests that Glygen’s DNA Purification TopTip technology is a method of choice for DNA/PCR purification, with superior recovery and efficiency (Manduzio, et al, ‘Comparison of Approaches for Purifying and Desalting Polymerase Chain Reaction Products Prior to Electrospray Ionization Mass Spectrometry’, Analytical Biochemistry March 15, 2010).
NuTip is Glygen’s revolutionary patented SPE cartridge in which chromatography material is embedded in the inner surface of a pipette tip. The sample is drawn into and out of the NuTip (using a micropipette or automation system), and the targeted molecule binds the complementary media for later elution. NuTip’s unique design maximises surface area in contact with the sample, and improves reproducibility due to lack of non-specific binding and backpressure.
TopTip, Glygen’s patented micro-spin column, is a uniquely modified tip that contains loose chromatography material and a 1-2 µm slit at the bottom. The sample is top-loaded into the TopTip, and is drawn through the chromatography material by spinning in a centrifuge; the fine slit permits liquid to pass through but retains chromatographic material.
Circle no. 260
from Brookhaven Instrument Corporation is a powerful new suite of software for multi detector, macromolecular characterisation and represents the most significant development in GPC/SEC analysis for many years. ParSEC is compatible with all GPC/SEC systems and detectors. Many attractive and practical features have been written into the new software for the benefit of chromatographers, including ‘function specific’ views, ensuring the uncluttered display of information relevant to the task at hand, customisable display and annotation options for all graph types, plus a Database approach for practical storage and archiving of data and results associated with any application.
ParSECTM Scanner of the Fourth Generation
A key benefit of ParSEC is that even though it is a powerful and fully featured software package, unlike other multi- angle, multi-detector software, it is very intuitive and remarkably easy to learn and use.
ParSEC is also capable of collecting and analyzing data from virtually all chromatographic detectors no matter whom the manufacturer is, making it the ideal ‘drop in’ replacement for all other GPC/SEC software currently available.
Circle no. 261
Camag presented the TLC Scanner of the fourth generation at this year’s Analytica in Munich, Germany, and since then has received very positive feedback. Users from different fields of application are convinced by the features of the new Scanner: improved signal to noise ratio, high sensitivity, and extended spectral range of 190 – 900nm. The new and modern design and the smaller footprint are equally appreciated. In addition the analyst benefits from easier loading of TLC/HPTLC plates in comparison with the predecessor TLC Scanner 3. Now the scanning table is brought into a user friendly load position at the push of a button. In combination with winCATS software the new TLC Scanner features rapid and precise measurement and evaluation of HPTLC chromatograms.
Circle no. 262
Free Capillary Gc Tips and Tricks Seminars
Simultaneous ESI/APCI Measurement for Improved Laboratory Throughout
Shimadzu has released a new report focusing on its LCMS- 2020 mass spectrometer and use of the DUIS-2020 Dual Ion Source probe for simultaneous ESI and APCI measurement.
In LCMS measurement, the time it takes to select the best ionization mode for each compound can negatively impact R&D efficiency. For researchers seeking a way to shorten the time required for analysis and method development, electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) probes can now be integrated without compromising either technique using Shimadzu’s Dual Ion Source DUIS-2020.
This application note presents results obtained following the analysis of a sample containing the water-soluble vitamins thiamine and riboflavin, and the fat-soluble vitamin calciferol. The research showed that good sensitivity is obtained for thiamine and riboflavin using ESI, but scarcely detected using APCI. Calciferol is detected using APCI, but ionization is insufficient with ESI. However, when the DUIS- 2020 is used, researchers can obtain well-balanced chromatograms. In conventional systems, the same analysis must be repeated after exchanging the ESI probe, but by using the DUIS-2020, the analysis is completed in a single test run. Because the LCMS-2020 uses the standard ESI probe as an integral component, upgrading to the DUIS- 2020 gives researchers a simple and cost-efficient way to improve laboratory throughput.
Circle no. 264 New Total Hydrocarbon Gas Analyser
AGC Instruments announces the latest addition to their product offering with a newly designed Total Hydrocarbon Gas Analyser. This new Gas Analyser represents the latest in digital and modern technology, and based on the Flame Ionisation Detector (FID) platform. Having evolved from the it’s well known predecessor and now with improved analytical performance, a greater market is being targeted. Utilising the finest tools available, the ease with which customers are able to achieve the best results has now been made easier. This new and easy to use THC Analyser has an interactive touch screen display where all controls and results are easily brought to the operator’s attention.
Designed to continuously measure and monitor ppm/ppb concentrations of Hydrocarbons in a gas stream. A heated version is also available for those samples where temperature must remain elevated. With a wide target audience including Air Separation Units, to Industrial Gas Production Sites to the Petrochemical and Refinery markets, AGC are aiming to further their presence and influence on the market.
A wide range of detection down to < 30ppb is achievable with an Autoranging function, other features include standard 4-20mA outputs with alarm contacts, data logging over RS 232, plus the results can be read direct on the display or transmitted to the Control Room or the DCS. Also new to this model is the validation of all results.
Circle no. 265
Crawford Scientific, in conjunction with Agilent Technologies, are staging a series of seminars across the UK informing customers about a myriad of tips and tricks that will improve their productivity when operating Capillary GC systems.
Hints on GC troubleshooting, column selection, how to move to ‘fast GC’ painlessly and methods employed to obtain best peak shape, resolution and detection limits are covered by the experienced speakers from Crawford’s and Agilent.
The seminar is intended to be the most productive event that chromatographers will attend this year and early registration is recommended. Each seminar lasts for a half day and registration is free. All registrants are entitled to lunch, free giveaways and special promotional offers.
For further Information on dates and venues and to register please visit
http://www.crawfordscientific.com Circle no. 263
INTERNATIONAL LABMATE - OCTOBER/NOVEMBER 2010 - CHROMATOGRAPHY & SPECTROSCOPY
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